Copa (NM_001134540) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR207971 representing NM_001134540 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTAACCAAATTCGAGACCAAAAGCGCGCGGGTGAAAGGCCTTAGTTTTCATCCTAAAAGACCCTGGA TCCTGACCAGTTTACATAATGGGGTCATCCAGTTATGGGACTATCGGATGTGCACCCTCATTGACAAATT TGATGAACATGATGGTCCAGTGCGTGGCATTGACTTCCATAAGCAGCAGCCCCTATTCGTCTCTGGAGGA GATGACTATAAGATTAAGGTTTGGAATTACAAACTTCGGCGCTGCCTCTTCACATTGCTTGGGCACTTGG ATTATATCCGCACGACGTTTTTTCATCATGAGTATCCTTGGATTCTGAGCGCCTCAGATGATCAGACCAT TCGGGTGTGGAACTGGCAGTCTAGAACCTGTGTCTGTGTGTTAACGGGGCACAACCATTATGTAATGTGT GCTCAGTTCCACCCCTCTGAAGACCTGGTGGTATCAGCTAGCCTGGACCAGACTGTCCGCGTTTGGGATA TTTCTGGTCTAAGGAAAAAAAACTTGTCTCCTGGTGCGGTGGAGTCCGATGTGAGAGGAATAACTGGGGT CGATCTGTTTGGAACTACAGATGCAGTGGTGAAGCATGTGCTAGAGGGCCATGACCGTGGAGTGAATTGG GCTGCCTTCCACCCGACTATGCCCCTTATTGTATCGGGGGCAGATGACCGGCAAGTGAAGATATGGCGCA TGAATGAATCAAAGGCATGGGAGGTTGACACCTGCCGGGGCCATTACAACAATGTGTCTTGTGCCGTCTT TCACCCTCGCCAAGAGTTGATCCTCAGCAATTCTGAGGACAAGAGTATTCGAGTCTGGGATATGTCTAAG CGGACTGGGGTTCAGACTTTCCGTAGGGATCATGATCGCTTCTGGGTCCTGGCTGCCCATCCCAACCTTA ACCTCTTTGCAGCAGGCCATGATGGTGGGATGATTGTATTTAAGCTGGAACGGGAGCGTCCAGCCTATGC TGTTCATGGCAATATGCTGCATTATGTGAAGGACCGATTCCTCCGTCAGCTGGATTTCAACAGCTCCAAA GATGTAGCTGTGATGCAGTTGCGGAGTGGTTCTAAGTTCCCAGTGTTCAACATGTCATACAATCCAGCAG AGAACGCCGTGCTGCTGTGCACCAGAGCAAGCAATTTAGAGAATAGTACCTATGATCTGTACACCATCCC CAAAGATGCAGACTCCCAGAATCCTGATGCCCCTGAAGGGAAACGATCCTCGGGCCTGACAGCGGTTTGG GTTGCTCGGAATCGGTTTGCTGTCCTAGATCGAATGCACTCGCTTCTGATTAAAAACCTGAAAAATGAGA TCACCAAGAAAATCCAGGTGCCCAACTGTGATGAAATCTTCTATGCTGGTACAGGCAACCTCCTGCTTCG GGATGCAGATTCCATCACACTCTTTGATGTCCAGCAGAAGCGAACTCTGGCATCAGTGAAGATTTCCAAG GTGAAATATGTTATCTGGTCGGCAGACATGTCTCATGTTGCACTCCTGGCCAAGCATGCCATTGTGATCT GTAACCGCAAGCTGGATGCTCTGTGTAACATTCATGAGAACATTCGTGTCAAGAGTGGGGCCTGGGATGA AAGTGGGGTGTTTATCTATACCACAAGCAACCACATCAAATATGCTGTCACCACTGGGGACCATGGGATC ATCCGAACTCTGGATTTGCCCATCTATGTCACAAGAGTGAAGGGCAATAACGTATACTGCCTGGACAGGG AGTGTCGTCCTCGGGTGCTCACCATTGACCCCACCGAATTCAAGTTCAAACTGGCCCTGATCAACAGGAA GTATGATGAGGTACTGCACATGGTGAGGAACGCCAAACTAGTAGGCCAGTCGATCATCGCTTACCTCCAG AAGAAGGGTTATCCTGAAGTGGCACTGCATTTTGTAAAGGATGAGAAAACTCGTTTCAGCTTGGCCCTGG AGTGTGGAAACATTGAGATTGCTCTAGAGGCAGCCAAAGCTCTGGATGACAAGAACTGCTGGGAGAAGCT GGGAGAAGTGGCCCTGTTACAGGGCAATCACCAGATTGTAGAAATGTGCTATCAGCGCACCAAAAACTTT GACAAGCTCTCCTTCCTGTACCTTATTACTGGCAACCTGGAGAAGCTTCGAAAGATGATGAAGATTGCTG AGATCCGAAAGGACATGAGTGGCCATTATCAGAATGCCCTGTACCTGGGTGATGTGTCAGAGAGAGTGCG AATCCTGAAGAACTGTGGGCAGAAATCCCTGGCCTACCTCACAGCTGCTACACATGGCTTAGATGAGGAA GCTGAGAGCCTGAAGGAGACATTTGACCCTGAGAAGGAGACAATCCCAGATATTGATCCTAATGCCAAGC TGCTCCAGCCACCAGCACCTATCATGCCATTGGATACCAATTGGCCCTTACTGACTGTGTCCAAAGGGTT CTTTGAAGGCTCCATTGCAAGCAAGGGGAAGGGAGGCGCGTTGGCTGCCGACATTGACATCGACACTGTT GGTACTGAAGGCTGGGGAGAGGACGCAGAGCTGCAGCTGGACGAAGATGGGTTTGTGGAGGCTCCGGAAG GTTTGGGGGAGGATACTCTCGGCAAGGGACAAGAGGAAGGAGGTGGCTGGGACGTAGAAGAAGATTTGGA GCTCCCACCTGAGCTGGATGTACCCTCTGGGGTTGCCGGTGGTGCTGAAGACGGTTTCTTTGTGCCCCCA ACCAAGGGAACAAGCCCAACTCAAATCTGGTGCAATAACTCCCAGCTTCCAGTGGATCACATCCTGGCAG GTTCTTTTGAAACAGCTATGCGGCTTCTTCATGACCAGGTGGGAGTAGTCCAGTTTGGCCCCTATAAGCA ACTGTTTCTACAGACATACGCGCGAGGCCGCACCACCTACCAAGCACTGCCCTGCTTGCCCTCCATGTAT AGCTACCCTAATCGCAATTGGAAGGACGCAGGGCTGAAGAATGGTGTGCCGGCTGTGGGCCTCAAGCTTA ACGACCTGATCCAGCGGCTGCAGCTCTGTTACCAGCTCAGCACAGTCGGCAAGTTTGAAGACGCTGTGGA GAAATTCCGCTCCATCCTACTCAGTGTGCCCCTTCTTGTGGTGGACAATAAACAGGAGATTGCAGAGGCC CAGCAGCTCATCACCATCTGCCGTGAGTACATTGTGGGTTTGTGCATGGAGATAGAAAGGAAGAAGCTAC CTAAGGAGACCCTAGACCAGCAGAAGCGCATCTGTGAGATGGCGGCTTATTTCACACACTCAAACCTGCA GCCTGTGCACATGATCCTGGTGCTGAGGACAGCCCTCAACCTCTTCTTCAAGCTCAAGAACTTCAAGACA GCTGCCACCTTTGCCCGACGCCTGCTGGAACTTGGGCCCAAACCTGAAGTAGCCCAACAGACAAGGAAAA TCTTGTCTGCCTGTGAGAAAAACCCCACAGATGCCTGCCAGCTCAATTATGACATGCACAACCCCTTTGA CATTTGTGCTGCATCTTACCGGCCCATCTACCGTGGAAAACCAGTGGAGAAGTGTCCACTCAGTGGGGCC TGCTATTCCCCTGAGTTCAAAGGGCAGATCTGCAGGGTCACTACAGTGACAGAGATTGGCAAAGATGTGA TTGGTCTGAGGATCAGTCCTCTTCAGTTTCGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR207971 representing NM_001134540 Red=Cloning site Green=Tags(s) MLTKFETKSARVKGLSFHPKRPWILTSLHNGVIQLWDYRMCTLIDKFDEHDGPVRGIDFHKQQPLFVSGG DDYKIKVWNYKLRRCLFTLLGHLDYIRTTFFHHEYPWILSASDDQTIRVWNWQSRTCVCVLTGHNHYVMC AQFHPSEDLVVSASLDQTVRVWDISGLRKKNLSPGAVESDVRGITGVDLFGTTDAVVKHVLEGHDRGVNW AAFHPTMPLIVSGADDRQVKIWRMNESKAWEVDTCRGHYNNVSCAVFHPRQELILSNSEDKSIRVWDMSK RTGVQTFRRDHDRFWVLAAHPNLNLFAAGHDGGMIVFKLERERPAYAVHGNMLHYVKDRFLRQLDFNSSK DVAVMQLRSGSKFPVFNMSYNPAENAVLLCTRASNLENSTYDLYTIPKDADSQNPDAPEGKRSSGLTAVW VARNRFAVLDRMHSLLIKNLKNEITKKIQVPNCDEIFYAGTGNLLLRDADSITLFDVQQKRTLASVKISK VKYVIWSADMSHVALLAKHAIVICNRKLDALCNIHENIRVKSGAWDESGVFIYTTSNHIKYAVTTGDHGI IRTLDLPIYVTRVKGNNVYCLDRECRPRVLTIDPTEFKFKLALINRKYDEVLHMVRNAKLVGQSIIAYLQ KKGYPEVALHFVKDEKTRFSLALECGNIEIALEAAKALDDKNCWEKLGEVALLQGNHQIVEMCYQRTKNF DKLSFLYLITGNLEKLRKMMKIAEIRKDMSGHYQNALYLGDVSERVRILKNCGQKSLAYLTAATHGLDEE AESLKETFDPEKETIPDIDPNAKLLQPPAPIMPLDTNWPLLTVSKGFFEGSIASKGKGGALAADIDIDTV GTEGWGEDAELQLDEDGFVEAPEGLGEDTLGKGQEEGGGWDVEEDLELPPELDVPSGVAGGAEDGFFVPP TKGTSPTQIWCNNSQLPVDHILAGSFETAMRLLHDQVGVVQFGPYKQLFLQTYARGRTTYQALPCLPSMY SYPNRNWKDAGLKNGVPAVGLKLNDLIQRLQLCYQLSTVGKFEDAVEKFRSILLSVPLLVVDNKQEIAEA QQLITICREYIVGLCMEIERKKLPKETLDQQKRICEMAAYFTHSNLQPVHMILVLRTALNLFFKLKNFKT AATFARRLLELGPKPEVAQQTRKILSACEKNPTDACQLNYDMHNPFDICAASYRPIYRGKPVEKCPLSGA CYSPEFKGQICRVTTVTEIGKDVIGLRISPLQFR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001134540
ORF Size	3672 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001134540.1, NP_001128012.1
RefSeq Size	4374 bp
RefSeq ORF	3675 bp
Locus ID	304978
MW	138.4 kDa

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