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Srgap2 (NM_001134958) Rat Tagged ORF Clone
CAT＃: RR207887
- TrueORF®
Srgap2 (Myc-DDK-tagged ORF) - Rat SLIT-ROBO Rho GTPase activating protein 2 (Srgap2), (10 ug)
"NM_001134958" in other vectors (3)
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CNY 10,930.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | RGD1566016 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR207887 representing NM_001134958
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGTCTCCAGCCAAATTCAAAAAGGATAAGGAGATCATCGCAGAGTATGATACTCAGGTCAAAGAGA TCCGGGCTCAGCTCACAGAGCAGATGAAATGCCTAGATCAGCAGTGCGAGCTCCGGGTGCAGCTGTTGCA AGACCTGCAGGACTTCTTCCGCAAGAAGGCTGAGATTGAGATGGACTACTCTCGCAACCTGGAGAAGCTA GCCGAGCGCTTTCTAGCCAAGACACGAAGCACTAAGGACCAGCAATTTAAGAAGGACCAGAATGTTCTCT CTCCAGTCAACTGCTGGAACCTCCTCTTAAACCAGGTGAAGCGGGAGAGCAGGGATCATACCACCCTGAG TGACATCTACCTGAATAATATAATTCCTCGATTTGTTCAAGTCAGCGAGGACTCAGGAAGACTCTTTAAA AAGAGTAAAGAAGTTGGCCAGCAACTCCAGGATGACTTGATGAAGGTCCTGAACGAGCTTTATTCGGTCA TGAAGACATATCACATGTACAATGCTGACAGCATCAGTGCTCAGAGCAAACTAAAGGAAGCAGAGAAGCA GGAAGAGAAGCAAATTGGCAAATCAGTAAAGCAAGAGGACCGGCAGACCCCTCGCTCCCCTGACTCCACG GCCAACGTCCGCATTGAGGAGAAACATGTCCGGAGGAGTTCAGTGAAGAAGATCGAGAAGATGAAGGAGA AGCGACAAGCCAAGTACACAGAGAACAAGCTGAAGGCCATTAAAGCCCGGAATGAGTATTTATTGGCTTT GGAGGCAACCAACGCATCTGTCTTCAAGTACTACATCCATGACCTGTCTGATATTATTGATCAGTGTTGT GACCTCGGCTACCATGCTAGCCTGAACCGGGCTCTACGCACTTTTCTGTCTGCTGAATTAAATCTGGAAC AATCAAAACATGAAGGTCTGGATGCTATTGAAAATGCAGTGGAAAACCTAGATGCCACCAGTGACAAGCA GCGGCTCATGGAGATGTACAATAATGTTTTCTGCCCCCCTATGAAGTTTGAATTCCAGCCCCACATGGGA GATATGGCCTCTCAGCTCTGTGCCCAGCAGCCTGTCCAGAGTGAGCTGGTGCAAAGACGCCAACAACTGC AGTCTCGCTTATCCACTCTGAAGATCGAGAATGAAGAGGTGAAAAAGACGATGGAGGCCACCCTGCAGAC CATACAGGACATCGTGACTGTTGAGGATTTTGATGTATCTGACTGCTTCCAGTATAGCAATTCCATGGAG TCTGTCAAATCAACTGTCTCAGAAACGTTCATGAGCAAGCCTAGCATTGCTAAGAGGAGAGCTAACCAGC AGGAGACGGAACAGTTCTATTTCACGAAAATGAAGGAGTACTTGGAGGGTAGGAACCTCATCACTAAATT ACAAGCCAAGCATGACCTCCTGCAGAAGACCCTGGGAGAAAGTCAGCGGACAGACTGTAGTCTTGCCAGG CGAAGCTCAACCGTGAGGAAACAGGACTCCAGCCAGGCAATTCCTCTGGTGGTAGAAAGCTGCATCCGGT TTATTAGCAGACATGGCCTACGACATGAGGGGGTTTTCCGGGTGTCTGGGTCACAAGTAGAAGTGAACGA CATAAAGAACGCCTTTGAGAGAGGAGAGGACCCCCTGGCTGGGGACCAGAATGACCATGACATGGACTCC ATAGCTGGCGTCCTCAAGCTTTACTTCCGGGGGCTGGAACACCCGCTCTTCCCCAAAGACATCTTCCATG ACTTGATTGCCTGTGTCACAATGGACAACCTGCAAGAGAGAGCTGTGCATATCCGGAAAGTCCTTCTGGT CCTGCCCAAGCCCACTCTGATTATCATGAGATACCTCTTTGCCTTCCTCAATCACTTATCACAGTTCAGT GAAGAGAACATGATGGACCCCTACAACCTTGCCATCTGCTTCGGGCCCTCACTGATGTCAGTGCCAGAGG GCCACGACCAGGTGTCCTGTCAAGCCCACGTGAATGAACTGATCAAAACCATCATCATCCAACATGAGAA CATTTTCCCAAATCCCAGGGAGCTGGAGGGTCCCATCTACAGCAGAGGAGGGAGCATGGAGGATTACTGT GACAGCACTCATGGAGAGACTATCTCTGCCGAAGACTCCACCCAGGACGTTACAGCGGAACACCACACAA GTGACGATGAGTGTGAGCCCATTGAAGCCATTGCCAAGTTTGACTACGTAGGCCGGACAGCCCGAGAACT GTCTTTCAAGAAGGGAGCATCCCTGCTGCTTTACCAGCGAGCCTCTGACGACTGGTGGGAAGGCCGGCAC AATGGTATAGACGGACTCATCCCCCATCAGTACATCGTAGTCCAAGACACCGAGGACGGTGTCGTGGAGA GGTCCAGCCCCAAGTCTGAGATTGAGGTCATGTCTGAGCCACCTGAAGAAAAGGTGACAGCCAGAACGGG GGCCAGCTGTCCCAGTGGGGGTCATGTAGCTGATATTTATCTTGCAAACATCAACAAAGGGATTCCTCGT TCGCCCGCCCATGAGCTGCCGCCTCCCCAAGAGGCACTCAGGCTATTGGAGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR207887 representing NM_001134958
Red=Cloning site Green=Tags(s) MTSPAKFKKDKEIIAEYDTQVKEIRAQLTEQMKCLDQQCELRVQLLQDLQDFFRKKAEIEMDYSRNLEKL AERFLAKTRSTKDQQFKKDQNVLSPVNCWNLLLNQVKRESRDHTTLSDIYLNNIIPRFVQVSEDSGRLFK KSKEVGQQLQDDLMKVLNELYSVMKTYHMYNADSISAQSKLKEAEKQEEKQIGKSVKQEDRQTPRSPDST ANVRIEEKHVRRSSVKKIEKMKEKRQAKYTENKLKAIKARNEYLLALEATNASVFKYYIHDLSDIIDQCC DLGYHASLNRALRTFLSAELNLEQSKHEGLDAIENAVENLDATSDKQRLMEMYNNVFCPPMKFEFQPHMG DMASQLCAQQPVQSELVQRRQQLQSRLSTLKIENEEVKKTMEATLQTIQDIVTVEDFDVSDCFQYSNSME SVKSTVSETFMSKPSIAKRRANQQETEQFYFTKMKEYLEGRNLITKLQAKHDLLQKTLGESQRTDCSLAR RSSTVRKQDSSQAIPLVVESCIRFISRHGLRHEGVFRVSGSQVEVNDIKNAFERGEDPLAGDQNDHDMDS IAGVLKLYFRGLEHPLFPKDIFHDLIACVTMDNLQERAVHIRKVLLVLPKPTLIIMRYLFAFLNHLSQFS EENMMDPYNLAICFGPSLMSVPEGHDQVSCQAHVNELIKTIIIQHENIFPNPRELEGPIYSRGGSMEDYC DSTHGETISAEDSTQDVTAEHHTSDDECEPIEAIAKFDYVGRTARELSFKKGASLLLYQRASDDWWEGRH NGIDGLIPHQYIVVQDTEDGVVERSSPKSEIEVMSEPPEEKVTARTGASCPSGGHVADIYLANINKGIPR SPAHELPPPQEALRLLEN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001134958 |
| ORF Size | 2574 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001134958.1, NP_001128430.1 |
| RefSeq Size | 3559 bp |
| RefSeq ORF | 2577 bp |
| Locus ID | 360840 |
| MW | 98.4 kDa |
| Gene Summary | RAC1 GTPase activating protein (GAP) that binds and deforms membranes, and regulates actin dynamics to regulate cell migration and differentiation. Plays an important role in different aspects of neuronal morphogenesis and migration mainly during development of the cerebral cortex. This includes the biogenesis of neurites, where it is required for both axons and dendrites outgrowth, and the maturation of the dendritic spines. Also stimulates the branching of the leading process and negatively regulates neuron radial migration in the cerebral cortex. May play a role for cognition, learning and memory. In non-neuronal cells, it may also play a role in cell migration by regulating the formation of lamellipodia and filopodia (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN207887 | Srgap2 (untagged ORF) - Rat SLIT-ROBO Rho GTPase activating protein 2 (Srgap2), (10 ug) |
CNY 10,930.00 |
|
| RR207887L3 | Lenti ORF clone of Srgap2 (Myc-DDK-tagged ORF) - Rat SLIT-ROBO Rho GTPase activating protein 2 (Srgap2), (10 ug) |
CNY 12,830.00 |
|
| RR207887L4 | Lenti ORF clone of Srgap2 (mGFP-tagged ORF) - Rat SLIT-ROBO Rho GTPase activating protein 2 (Srgap2), (10 ug) |
CNY 13,970.00 |
