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Gas8 (NM_001039030) Rat Tagged ORF Clone
CAT＃: RR207882
- TrueORF®
Gas8 (Myc-DDK-tagged ORF) - Rat growth arrest specific 8 (Gas8), (10 ug)
"NM_001039030" in other vectors (3)
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CNY 6,180.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MGC124779 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR207882 representing NM_001039030
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACCCAAAAGGAGAGGGAAGAAAGGGAAAGCCAAAGGGAATGCAGTCGTTGATGGGGTCGCACCAG AGGACATGTCCAAGGAGCAGGTGGAGGAACATGTCGCCCGCATCCGGGAGGAGCTGGACCGGGAGCGGGA GGAGCGCAACTACTTTCAGCTGGAGCGGGACAAAATCCACACCTTCTGGGAGATCACGCGGAGGCAGCTG GAGGAGAAGAAGGCTGAGCTGCGCAACAAAGACCGGGAGATGGAGGAGGCCGAGGAGAGGCACCAGGTGG AGATCAAGGTTTACAAGCAGAAAGTGAAGCACCTTCTGTACGAACACCAGAACAATCTGGCAGAGGTGAA GACTGAGGGCACCGTGGTCATGAAGCTGGCCCAGAAGGAGCACCGCGCCCAAGAGGGCGCTCTGCGCAAG GACATGCGCGTGCTGAAAGTGGAGCTGAAGGAGCAGGAGCTGGCTAACGAGGTGGTGATAAAGAACCTGC GTCTGAAACAAGCCGAGGAGATCACCAAGATGCGAAATGACTTTGAAAGGCAAGTTCGAGAAATCGAGGC CAAATATGATAAAAAGATGAAGATGCTTCGAGACGAGCTTGACCTGCGGAGAAAGACTGAGATCCACGAG GTGGAGGAGAGGAAGAACGGGCAGATCAGTACGCTGATGCAGCGTCATGAGGAGGCCTTCACTGACATCA AAAACTACTACAATGACATCACTCTCAACAACCTGGCCCTCATCAATTCCCTCAAGGAGCAGATGGAGGA TATGAGGAAGAAGGAGGAGTACCTGGAGAGGGAGATGGCGGAGGTGTCTCTGCAGAACAAGCGGCTGGCT GAGCCGCTGCAGAGAGCCAAGGAGGAGATGAGCGAGATGCAGAAGAGACTGGGGAACCATGAGAGGGACA AGCAGATCCTGGCCTGTACGAAGGCACGTTTGAAGGTCACTGAGAAGGAGCTGAAGGACCTGAAGTGGGA GCATGAGATCCTGGAGCAGAGGTTCATCAAGGTGCAGCAGGAGAGGGAAGAGCTCTATCGGAAGTTCACT GCAGCCATCCAGGAGGTGCAGCAGAAGACAGGCTTCAAGAACCTGGTGCTGGAGCGTAAGCTGCAGGCAC TGAATGCTGCTGTGGAGAAGAGAGAGGTGCAGTTCAATGAGGTGCTGGCTGCCTCCAACCTGGACCCTAC AGCACTAACACTTGTGTCTCGCAAACTTGAGGACGTTCTAGAGTCGAAGAATACCACCATCAAGGACTTG CAGTATGAGCTGGCCCGAGTCTGCAAGGCCCACAACGACCTGTTGCGTACATATGAGGCAAAGTTATTGG CCTTCGGGATCCCCCTGGACAATGTGGGGTTCAAGCCCCTGGAGACAGCAGTGATTGGACAGACACTGGG ACAGGGCCCTGCAGGACTTGTGGGTGCGCCGACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR207882 representing NM_001039030
Red=Cloning site Green=Tags(s) MAPKRRGKKGKAKGNAVVDGVAPEDMSKEQVEEHVARIREELDREREERNYFQLERDKIHTFWEITRRQL EEKKAELRNKDREMEEAEERHQVEIKVYKQKVKHLLYEHQNNLAEVKTEGTVVMKLAQKEHRAQEGALRK DMRVLKVELKEQELANEVVIKNLRLKQAEEITKMRNDFERQVREIEAKYDKKMKMLRDELDLRRKTEIHE VEERKNGQISTLMQRHEEAFTDIKNYYNDITLNNLALINSLKEQMEDMRKKEEYLEREMAEVSLQNKRLA EPLQRAKEEMSEMQKRLGNHERDKQILACTKARLKVTEKELKDLKWEHEILEQRFIKVQQEREELYRKFT AAIQEVQQKTGFKNLVLERKLQALNAAVEKREVQFNEVLAASNLDPTALTLVSRKLEDVLESKNTTIKDL QYELARVCKAHNDLLRTYEAKLLAFGIPLDNVGFKPLETAVIGQTLGQGPAGLVGAPT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001039030 |
| ORF Size | 1434 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001039030.1, NP_001034119.1 |
| RefSeq Size | 1602 bp |
| RefSeq ORF | 1437 bp |
| Locus ID | 361438 |
| UniProt ID | Q499U4 |
| MW | 56.3 kDa |
| Gene Summary | Component of the nexin-dynein regulatory complex (N-DRC), a key regulator of ciliary/flagellar motility which maintains the alignment and integrity of the distal axoneme and regulates microtubule sliding in motile axonemes. Plays an important role in the assembly of the N-DRC linker. Plays dual roles at both the primary (or non-motile) cilia to regulate hedgehog signaling and in motile cilia to coordinate cilia movement. Required for proper motile cilia functioning. Positively regulates ciliary smoothened (SMO)-dependent Hedgehog (Hh) signaling pathway by faciltating the trafficking of SMO into the cilium and the stimulation of SMO activity in a GRK2-dependent manner.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN207882 | Gas8 (untagged ORF) - Rat growth arrest specific 8 (Gas8), (10 ug) |
CNY 6,180.00 |
|
| RR207882L3 | Lenti ORF clone of Gas8 (Myc-DDK-tagged ORF) - Rat growth arrest specific 8 (Gas8), (10 ug) |
CNY 7,980.00 |
|
| RR207882L4 | Lenti ORF clone of Gas8 (mGFP-tagged ORF) - Rat growth arrest specific 8 (Gas8), (10 ug) |
CNY 8,840.00 |
