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Alkbh3 (NM_001014180) Rat Tagged ORF Clone
CAT＃: RR207832
- TrueORF®
Alkbh3 (Myc-DDK-tagged ORF) - Rat alkB, alkylation repair homolog 3 (E. coli) (Alkbh3), (10 ug)
"NM_001014180" in other vectors (3)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR207832 representing NM_001014180
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGACAAGAGACAGCGAGCCCGAGTGCAGGGAGCCTGGGCCACACCTACCAAGAGCCAGTCTGCTG CTCGGCCAGCTACTCCTGCTAGAAGCCGTCCTTCCCAGACACCAGGGCCAAGCTGGAGGAGCAAGGAACA GCAGCAATGTGACCGACGGTTTGTGTTCAAAGAACCGCAGCTGGTTGTACGTGCAGCCCCAGAGCCCAGA GTGATTGACAGGGAGGGTGTGTACGAAATCAGCCTGTCCCCTACAGGTGTGTCTAGGGTGTGTTTATATC CTGGCTTTGTGGACTTGAAGGAAGCTGACTGGATCTTGGAGCGGCTTTGTCAAGATGTCCCCTGGAAACA GAGGATGGGCATCAGAGAGGATATAACTTATCCGCAACCAAGACTTACAGCATGGTATGGAGAGCTTCCT TACACTTACTCAAGAGTCACTATGGAACCAAATCCTCACTGGCTTCCTGTGCTGTGGACTCTGAAGAGCC GCATTGAGGAGAACACTGGCCACACCTTCAACTCCTTGCTGTGTAATTTTTACCGGGACGAGAAGGACAG TGTGGACTGGCACAGCGATGATGAGCCATCCCTGGGGAGCTGCCCTGTCATTGCTTCACTCAGTTTTGGT GCCACTCGGACTTTTGAGATGAGGAAGAAACCCCCACCTGAAGAGAATGGAGACTATACTTATGTGGAGA GAGTGAAGATACCATTGGATCACGGGACCTTGCTAATCATGGAAGGAGCCACGCAAGCTGACTGGCAGCA CCGAGTGCCCAAGGAATACCACTCCAGAGAACGGAGAGTAAACCTAACCTTTCGGACCGTTTATCCAGAC CCAAGAGGAGCCCCCGGTGATACTTCTGCAGAGCTGCCACTGAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR207832 representing NM_001014180
Red=Cloning site Green=Tags(s) MGDKRQRARVQGAWATPTKSQSAARPATPARSRPSQTPGPSWRSKEQQQCDRRFVFKEPQLVVRAAPEPR VIDREGVYEISLSPTGVSRVCLYPGFVDLKEADWILERLCQDVPWKQRMGIREDITYPQPRLTAWYGELP YTYSRVTMEPNPHWLPVLWTLKSRIEENTGHTFNSLLCNFYRDEKDSVDWHSDDEPSLGSCPVIASLSFG ATRTFEMRKKPPPEENGDYTYVERVKIPLDHGTLLIMEGATQADWQHRVPKEYHSRERRVNLTFRTVYPD PRGAPGDTSAELPLR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001014180 |
| ORF Size | 885 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001014180.1, NP_001014202.1 |
| RefSeq Size | 1286 bp |
| RefSeq ORF | 888 bp |
| Locus ID | 362169 |
| UniProt ID | Q5XIC8 |
| MW | 34 kDa |
| Gene Summary | Dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A). Repairs alkylated DNA containing 1-methyladenosine (m1A) and 3-methylcytosine (m3C) by oxidative demethylation. Has a strong preference for single-stranded DNA. Able to process alkylated m3C within double-stranded regions via its interaction with ASCC3, which promotes DNA unwinding to generate single-stranded substrate needed for ALKBH3. Also acts on RNA. Demethylates N(1)-methyladenosine (m1A) RNA, an epigenetic internal modification of messenger RNAs (mRNAs) highly enriched within 5'-untranslated regions (UTRs) and in the vicinity of start codons. Requires molecular oxygen, alpha-ketoglutarate and iron.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN207832 | Alkbh3 (untagged ORF) - Rat alkB, alkylation repair homolog 3 (E. coli) (Alkbh3), (10 ug) |
CNY 3,990.00 |
|
| RR207832L3 | Lenti ORF clone of Alkbh3 (Myc-DDK-tagged ORF) - Rat alkB, alkylation repair homolog 3 (E. coli) (Alkbh3), (10 ug) |
CNY 6,080.00 |
|
| RR207832L4 | Lenti ORF clone of Alkbh3 (mGFP-tagged ORF) - Rat alkB, alkylation repair homolog 3 (E. coli) (Alkbh3), (10 ug) |
CNY 6,650.00 |
