Recql4 (NM_001130494) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR207699 representing NM_001130494 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCGGCTTGCGACCGTTCGCGCGTGGCTGCAGGATTGGGAACGCGCTTTTGTACGGCTGCACGGGC GACGGCCAGCAAAGGAGGATGTGGAGGCGGCACCCGAGGAGACCCGCGCGCTCTACCGTGAGTACCGTAA CCTAAAGCAGGCAGTGAGTCAGGCCGACGACGGACACCGCGTCCAAAAGCAATCACTTGCCAAGGCAGCT GAGGAGGAACAGGAGCCAAGCTGCTGGGGTTCCCACCTGAACCGAGCTGCAACCCAGAACACACAGTCTA TTCCAAAGCAGAGCCCACTGAGTTCTATACAAGACTATGGGAAGAGGCTCAAAGCCAATCTTAAAAACAC ACTACAGGGCGGGCCAACCCTGAGTCGAAAACTCCAGCATCAGAAGAGATCCTTGTCCATAGTGCCCACA TCAAGGCCACCAGGCCCAAGGACTGAATCCCCCTGTCCAGAACAAGCTAATGATGCTCTTCCCCAGGTTC CTGTGCCCCAGCCAAGGCAAGGCCAACTCCAGAAGCTCCAGTCATCCCTGAGCCTGAGGTTGAGTTCCCT AGACCCTGGTTGGTTAGAGAGGTGTCAAAACAGAGTTTCAGATCCTCTAGAGGTTCCAGGTGCTTGTGGG CTTGACCTGAGTGCAAAGGAGTCACAACCTCAGATGTCAGGCAAGGTGAATGTCATTAGTCCTGACATCC TTTCAGAAGTATCTTCACAGAGCCCACAGGCCCTAGCCCAACAGCCAGCCCAAGTTTTGTCACAGATCCC CAAATCCAGCAACAGTAAAGACAGGAAGCGGAAACGGAATGAGAAGGGGGAGGACTTTGCACAAGCCCAG CCCAGCAGCCAAGCAGGACCCCTGTCTGAGGGAGCTGGGGCTACAGCACATGGGCAAGGCCCTCCAGGAG AACCCACACAAGTGACTGTCCCTCAGCACCGCTGCTCTTCAAACCAGGCCAGGACAGAGAATGCTAAGAG TGCGGCCCACCTCCCCATGTCCCCTCGGCCAGCTTCCCTAGACAGAGGGAACTATGTTCGACTTAACATG AAAACCAAATGCTTTGTACGAGTTGGGGCCAGTCGGGGCAGGCTTCTCCGAAAGCAGGTATGGAAGCAAA AGTGGAAGAAGAAACAAGAGTGGTTTGGGAAAAGTGGACCCAGGGCCACAGTCAAGGACACTTGTTTCCG GTGTGGGCAGTTTGGTCACTGGGCATCCCAGTGTTCCCAACCAGGCTCCACCCTGACCATCCAGCCAAAA GGTGACAGGAATGACAAACATCCCATTTCCACGTTGGGGGGAGTAGCACAGAAGACTGGCACCACTTCCT GTAACCACTCTGGTGAGGAAACTGAACCTGCTGGACCAGTGCTACCGGTGCCTCATTGCCCCACGCCAGT GTTACCCCTCTACCCACCAGGGCCCTTGGGACAGGTAGCAGACACCCCTGCTGAGGTATTCCAAGTCCTG GAGCAGCTAGGGCACCACACCTTCCGCCCTGGGCAAGAGCGTGCTGTCATGAGGATTCTTTCTGGCATCT CCACACTGTTAGTGTTGCCCACGGGTGCTGGCAAGTCTCTGTGCTACCAGCTTCCTGCACTGCTCTATGC CAAGCGAAGCCCCTGTCTCACACTGGTCGTCTCTCCTCTCCTGTCACTCATGGATGACCAGGTGTCTGAT CTGCCTTCATGTCTGAAGGCGGCCTGCCTCCACTCAGGAATGACCAAGAAACAACGAGAATCTGTCTTGA AGAAGGTACGGGCAGCCCAGGTGCATGTGCTGATTGTGTCCCCAGAGGCCTTGGTGGGATGCGGGGCTAA GGGTCCTGCCAACCTCCCCCCGGCCGCTCAGCTGCCTCCAGTCGCCTTTGCCTGCATTGATGAGGTCCAC TGCCTCTCTCAGTGGTCACATAACTTCCGGCCCTGCTACTTACGTGTTTGTAAAGTACTTCGGGAGCATA TGGGTGTGCGCTGCTTTTTGGGTCTCACAGCCACAGCCACACGAAGCACTGCTCGTGATGTGGCCCAGCA CCTGGGCATAGCTGAAGAGCTTGAGGTCAGTGGGTCAGCCAGCATCCCTGCCAATCTGCACCTCTCCGTG TCCATGGATCGAGACTCAGACCAGGCTCTGGTGACACTGCTACAAGGTGACCGCTTCCGTACCCTGGATT CGATTATCATTTACTGTGCTCGACGAAAGGACACAGAGCGGGTGGCTGCCCTCCTCCGGACCTGCCTGTC CACAGTGAGAGATTCAAAGCCAAGAGGCCGTGGCCCTGAGACTTTAGCGGAAGCATACCATGCTGGCATG TGTAGCCAGGAACGGAAACGAGTACAACAGGCCTTCATGCAGGGCCACCTGCGCATGGTAGTGGCCACAG TGGCATTTGGGATGGGACTGGACCGTCCAGATGTTCGGGCTGTGCTACACCTGGGACTGCCTCCAAGCTT CGAGAGCTACGTGCAAGCTATTGGCCGTGCAGGACGTGATGGGAAGCCTGCCCATTGCCACCTGTTCCTG CATCCTCAGGGTGAAGACCTCTGGGAACTGAGCAGACATGTCCATGCTGATAGCACTGACTTCCTGGCTG TGAAGAAGCTGGTACAGCGTGTGTTCCCAGCCTGCTCATGCGCCCAGAGGCCTATGTCCCCACTTGAGGA AGTCAAAGAGCACAGTGGCCAACAAACATACCCTGTACTGGGACTAGCCTGCCGGGGCCATAAGCGGGCA CTCCCAGTGCAGTCTACAGTACTGGCTCTGGACATGAAAGAGGAGGCCATTGAGACTTTGCTGTGCTATT TGGAACTACACCCTCGGCACTGGTTGGAGCTGCTGCCATACACCTATGCCCACTGCCATCTGCGTTGCCC TGGGGGCAGTGCCCAGCTGCAAGCTCTGGTCCACAGGTGTCCCCCTTTGGCTGCATGCTGGGCCAAGTGG CCACCTAAAGACACAAGTCAGGGTAGCAGCGCCTTAGAGTTTGATGTGGTGGAACTGGCAGACTCCATGG GCTGGGAGTTGGCCTCTGTACGGCAGGCTCTCCACCAGCTGAAGTGGGACCCAGAGCCAAAGAAAGGTGC ACCACAGGGCACAGGAGTGCTTGTGGAGTTCAGTGAGTTGGCCTTTCACCTGCACAGTCGTGGGGACCTG ACAGATGAGGAGAAGGACCAGATCTGTGACTTTCTGTACAGCCGTGTGCAGGCTCGTGAACACAAGGCCC TGGCCCACCTACACCAAATGTCCAAGGCCTTTCAAAGTGTAGCATTTCCCAGTTGTGGACCCTGTTTAGA GCAGTCTGACGAGGGGCACAGCAATCGGGTGAAGACCCTGGTCAGCTACTACTTTGAGGAGGAGGAGGAG GAGGAAGAGGAAGAAACTATGAAGGATACTCAGGATCCAAAACCTGGGCAGACCCAGCTTCAGGATTGGG AGGACCAAGTTCGCCGCGATGTCCACCAGCTCCTGTCCCTGAGGCCAGAAGAAAAGTTTTCAGGAAGGGC TGTGGCCCGCATCTTCCATGGAATTGCAAGTCCATGCTACCCAGCCCAGGTGTTTGGGCAGGACCGGCGC TTCTGGAGAAAGTACCTACACCTGGACTTCCACGCCCTCATGCACCTAGCCACAGAGGAGCTCCTGCTCA GAGGCCGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR207699 representing NM_001130494 Red=Cloning site Green=Tags(s) MERLATVRAWLQDWERAFVRLHGRRPAKEDVEAAPEETRALYREYRNLKQAVSQADDGHRVQKQSLAKAA EEEQEPSCWGSHLNRAATQNTQSIPKQSPLSSIQDYGKRLKANLKNTLQGGPTLSRKLQHQKRSLSIVPT SRPPGPRTESPCPEQANDALPQVPVPQPRQGQLQKLQSSLSLRLSSLDPGWLERCQNRVSDPLEVPGACG LDLSAKESQPQMSGKVNVISPDILSEVSSQSPQALAQQPAQVLSQIPKSSNSKDRKRKRNEKGEDFAQAQ PSSQAGPLSEGAGATAHGQGPPGEPTQVTVPQHRCSSNQARTENAKSAAHLPMSPRPASLDRGNYVRLNM KTKCFVRVGASRGRLLRKQVWKQKWKKKQEWFGKSGPRATVKDTCFRCGQFGHWASQCSQPGSTLTIQPK GDRNDKHPISTLGGVAQKTGTTSCNHSGEETEPAGPVLPVPHCPTPVLPLYPPGPLGQVADTPAEVFQVL EQLGHHTFRPGQERAVMRILSGISTLLVLPTGAGKSLCYQLPALLYAKRSPCLTLVVSPLLSLMDDQVSD LPSCLKAACLHSGMTKKQRESVLKKVRAAQVHVLIVSPEALVGCGAKGPANLPPAAQLPPVAFACIDEVH CLSQWSHNFRPCYLRVCKVLREHMGVRCFLGLTATATRSTARDVAQHLGIAEELEVSGSASIPANLHLSV SMDRDSDQALVTLLQGDRFRTLDSIIIYCARRKDTERVAALLRTCLSTVRDSKPRGRGPETLAEAYHAGM CSQERKRVQQAFMQGHLRMVVATVAFGMGLDRPDVRAVLHLGLPPSFESYVQAIGRAGRDGKPAHCHLFL HPQGEDLWELSRHVHADSTDFLAVKKLVQRVFPACSCAQRPMSPLEEVKEHSGQQTYPVLGLACRGHKRA LPVQSTVLALDMKEEAIETLLCYLELHPRHWLELLPYTYAHCHLRCPGGSAQLQALVHRCPPLAACWAKW PPKDTSQGSSALEFDVVELADSMGWELASVRQALHQLKWDPEPKKGAPQGTGVLVEFSELAFHLHSRGDL TDEEKDQICDFLYSRVQAREHKALAHLHQMSKAFQSVAFPSCGPCLEQSDEGHSNRVKTLVSYYFEEEEE EEEEETMKDTQDPKPGQTQLQDWEDQVRRDVHQLLSLRPEEKFSGRAVARIFHGIASPCYPAQVFGQDRR FWRKYLHLDFHALMHLATEELLLRGR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001130494
ORF Size	3648 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001130494.1, NP_001123966.1
RefSeq Size	3860 bp
RefSeq ORF	3651 bp
Locus ID	300057
MW	134.7 kDa

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