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Fbl (NM_001025643) Rat Tagged ORF Clone
CAT＃: RR207477
- TrueORF®
Fbl (Myc-DDK-tagged ORF) - Rat fibrillarin (Fbl), (10 ug)
"NM_001025643" in other vectors (3)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MGC116371 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR207477 representing NM_001025643
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCCAGGTTTCAGCCCCCGTGGGGGCGGCTTTGGTGGCAGAGGCGGCTTTGGTGACAGAGGTGGTA GAGGAGGAGGCCGAGGAGGGAGAGGAGGTTTTGGCGGTGGACGAGGAGGCTTTGGCGGTGGAGGTCGAGG TCGAGGTGGAGGAGGCGGTGGCTTCAGGGGACGAGGAGGTGGAGGTGGACGAGGTGGAGGCTTCCAGTCT GGGGGCGGCCGGGGTCGAGGTGGTGGCCGGGGAGGCAAGAGAGGAAACCAGTCAGGGAAGAATGTGATGG TGGAGCCCCATCGGCATGAAGGAGTCTTTATCTGTCGCGGAAAGGAGGATGCCCTTGTCACAAAGAATCT GGTTCCTGGAGAATCTGTGTATGGAGAGAAGAGAGTCTCTATCTCCGAAGGAGATGACAAAATTGAGTAC CGAGCCTGGAACCCCTTCCGCTCCAAGCTGGCCGCAGCAATCCTGGGTGGCGTAGACCAGATCCACATCA AGCCGGGGGCCAAGGTGCTCTACCTTGGGGCAGCCTCAGGCACCACCGTCTCCCACGTGTCTGACATTGT TGGCCCGGATGGTCTGGTCTACGCAGTTGAGTTCTCCCACCGCTCTGGCCGTGACCTCATCAACTTGGCC AAGAAGAGGACCAACATTATTCCTGTAATTGAAGATGCTCGGCACCCACACAAATACCGCATGCTTATTG CAATGGTGGATGTCATCTTTGCCGATGTGGCCCAGCCAGACCAAACCCGAATTGTGGCCCTGAATGCCCA CACCTTCCTGCGGAATGGAGGACACTTTGTGATTTCCATTAAGGCCAACTGCATTGACTCCACAGCCTCA GCAGAAGCTGTGTTTGCATCTGAAGTGAAAAAGATGCAGCAAGAGAACATGAAGCCTCAGGAGCAGTTGA CACTAGAGCCTTATGAACGAGACCACGCTGTGGTTGTAGGTGTGTACAGGCCACCTCCCAAGGCGAAGAA C ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR207477 representing NM_001025643
Red=Cloning site Green=Tags(s) MKPGFSPRGGGFGGRGGFGDRGGRGGGRGGRGGFGGGRGGFGGGGRGRGGGGGGFRGRGGGGGRGGGFQS GGGRGRGGGRGGKRGNQSGKNVMVEPHRHEGVFICRGKEDALVTKNLVPGESVYGEKRVSISEGDDKIEY RAWNPFRSKLAAAILGGVDQIHIKPGAKVLYLGAASGTTVSHVSDIVGPDGLVYAVEFSHRSGRDLINLA KKRTNIIPVIEDARHPHKYRMLIAMVDVIFADVAQPDQTRIVALNAHTFLRNGGHFVISIKANCIDSTAS AEAVFASEVKKMQQENMKPQEQLTLEPYERDHAVVVGVYRPPPKAKN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001025643 |
| ORF Size | 981 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001025643.1, NP_001020814.1 |
| RefSeq Size | 1208 bp |
| RefSeq ORF | 984 bp |
| Locus ID | 292747 |
| UniProt ID | P22509 |
| MW | 34.2 kDa |
| Gene Summary | S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN207477 | Fbl (untagged ORF) - Rat fibrillarin (Fbl), (10 ug) |
CNY 3,990.00 |
|
| RR207477L3 | Lenti ORF clone of Fbl (Myc-DDK-tagged ORF) - Rat fibrillarin (Fbl), (10 ug) |
CNY 6,080.00 |
|
| RR207477L4 | Lenti ORF clone of Fbl (mGFP-tagged ORF) - Rat fibrillarin (Fbl), (10 ug) |
CNY 6,650.00 |
