Nup210 (NM_053322) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Pom210
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR207371 representing NM_053322 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCGGGCCTCGCTGATACAGCCGGGGCTCTGGGCGCTGCTGTTGCTGCAGGCAGTGGGCCCCGCCG TGGCCGCCAAGCTTAACATTCCCAAGGTGCTGCTGCCCTTTACGCGAGCCACGCGCGTGAACTTCACTCT GGAAGCTTCTGAGGGCTGCTACCGCTGGTCATCCACACGGCCAGAGGTAGCCAGCATAGAGCCATTGGGC TCTAGTGAACAGCAGTGCTCCCAGAAGGCAGTGGTGCAGGCCCGCCTGACCCAGCCAGCCCGCCTGACCA GCATCATCTTTGCAGAAGACATCACTACAGGCCAGGTGCTGCGCTGCGACGCCATTGTTGATCTCATCCA CGGCATTCAGATTGTCTCTACCACCCGTGAGCTCTATCTTGAGGACTCTCCGCTGGAGCTGAAGATCCAG GCTCTGGACTCAGAAGGGAATACCTTCAGTACCCTGGCAGGCCTGGTCTTTGACTGGACAATTGTGAAGG ATACAGAGGCCAATGGATTCTCAGACTCCCACAATGCACTACGAATCCTCACCTTCTTGGAATCTACATA TATCCCGCCTTCCTACATCTCAGAGATGGAGAAGGCAGCCAAACAAGGCGACACCATCCTGGTGTCTGGG ATGAAGACTGGAAGCTCCAAGCTCAAGGCTAGAATTCAGGAGGCTGTCTACAAGAATGTTCGCCCTGCGG AAGTCAGGCTGCTGATCTTGGAGAACATCCTTCTGAATCCAGCCTATGATGTTTACCTACTGGTGGGGAC GTCCATTCACTACAAGGTTCAGAAGATCAGGCAGGGCAAGATTACAGAACTCTCAATGCCTTCCGATCAG TACGAGCTGCAGCTCCAGAACAGCATCCCAGATCCCCAGGGAGACCCTGCCCGTCCTGTGGCCGTCCTGA CCCAGGACACATCAAGAGTCACTGCCATGCAGATGGGACAGAGCAACCTGGTCCTGGGCCACAGGAGCAT CCGTATGCAAGGAGCTTCTAGGCTGCCCAACAGCACCATCTATGTGGTTGAAGCCGGGTACCTAGGCTTC ACAGTTCACCCTGGTGACAGGTGGGTGCTTGAGACTGGACATCTGTATGCAGTTACCATCGAAGTATTTG ATAGATCCAGCAACAAGGTCTACCCGTCTGACAACATCCGAATTGAAGCTGTGTTTCCTGCTGAGTTTTT TGAGGTTCTGTCATCTTCCCAGAATGGGTCTTATCATCATGTCAGGGCTATCCAGAGTGGCCAGACGACC ATCAGTGCAAGCCTCACCTCTGTGGTGGACCAGGATGGTGGAGTCCATGTGCTTCAGGTACCTGTGTGGA ACCAGCAAGAAGTAGACATTCATATACCCATCACACTCTATCCCAGCATCCTGACGTTTCCATGGCAACC AAAGACAGGAGCCTATCAATATACTATAAAGGCCCATGGGGGCAGTGGGAACTTCACCTGGTCTTCCTCG AGCTACATGGTTGCCACGGTTACTGTCAAGGGTGTCATGACCACAGGCGGTGACACTGGCCTCAGTGTGA TCCGGGCACATGATGTTCAGAACCCTCTCCATTTTGGTGAGATGAAGGTGTATGTGATTGAGCCCAGCAG CATGGAGTTTGCCCCGTGCCAGGTGGAGGCTCGAGTGGGTCACACCCTGGAGCTGCCCCTGACAATCAGT GGCCTCATGCCCGGTGGGAGCAGCGAGGTGGTCACCCTGAGCGACTGCTCCCATTTTGACCTGGTGGTGG AGGTGGAGAACCAGGGTGTGTTCCAGCCATTGCCAGGGAGGCTGCCACCAGGGCCTGAGCACTGCAGTGG AGTCAAAGTGAGAGCTGATGCCCAGGGGTCCACTACCCTCCTTGTCAGCTACACACATGGCCATGTGCAC TTGGGTGCAAAGATCACTCTTGCTGCCTACCTGCCCCTCAAGGCTGTGGATCCCTCTTCTGTTGCTGTGG TAACCCTGGGATCCTCAAAGGAGATGCTGTTTGAAGGAGGCCCCAGGCCATGGGTCCTGGAACCTTCCAA ATTCTTTCGGAATGTCACTTCTGAAGACACAGGCAGCATCAGTCTCTCTCTCTTGGGTCCTCCAGCCTCT CGGAATTACCAGCAGCACCGGGTCCTTGTGACCTGCCAGGCCCTGGGTGAGCAGGTCATCGCCCTGTCTG TGGGGAACCGGCCCAGCCTCTCTAACCCCTTCCCTGCTGTGGAGCCCACTGTGGTGAAATCCGTCTGCGC CCCACCGTCCAGGCTTACTCTCATGCCTGTCTATGCCCTCCCGCAGCTCGACCTCTCTTGTCCATTGCTG CAGCAGAATAAGCAGGTGGTTCCAGTATCCAGTCACCGCAATCCCCTGTTAGACCTGGGTGCTTATGACC AACAAGGCCGGCGATTCGATAACTTCAGCTCTCTGAGCATCCAGTGGGAGTCCTTTCGGCCACTGCTGGC CAGCATTGAGGTTGACCAGCCCATGCAGCTGGTATCCCAGGATGATGGGAATGGCCAAAAGAAACTACAT GGTTTGCAGACAGTTTCGGTTCACGAGGCATCAGGAACCACAGCCATCTCTGCCACGGCGACTGGCTACC AGCAGTCCCACCTGAGTGCAGCCGGAGTGAAGCAGCTGCGTGACCCTCTAGTACCTGTGTCAGCCTCCAT TGAGCTTATACTCGTAGAGGATGTGAGGGTCAGTCCAGAAGAAGTGACCATCTATAACCATCCTGGTGTC CAGGTAGAACTCCACATCACAGAAGGCTCTGGCTATTTCTTCCTCAACACAAGCACCCAGGACATCATCA ATGTGGCCTACCAGGACACCCGGGGCGTGGCTATGGTGCATCCTCTGTTCCCAGGCTCATCAACTGTCAT GGTCCATGACTTGTGCCTGACCTTCCCAGCCCCAGCAAAGGCCACCATTCATGTCTCCGACATCCAGGAG CTCTATGTCCGAGTGGTGGACAAGGTGGAGATCGGGAAGGCTGTCAAGGCCTATGTCCGTGTGTTGGACT TCTACAAGAAGCCTTTCCTGGCCAAGTACTTCACCTTTATGGACCTGAAGCTGCGAGCAGCCTCCCAGAT CATCACGCTGGTGACTCTGGATGAAGCTCTAGACAATTACACGGCCACATTCCTTGTGCACGGCGTGGCC ATTGGCCAAACCAGCCTGTCGGCAAGTGTGACGGACAAATCTGGGCAGAGAGTCAGCTCCACCGCACAAC AGATCGAGGTCTTCCCCCCATTCAGACTGATACCAAGGAAGGTGACACTGATTATTGGAGCCATGATACA GATCACCTCCGAGGGCGGCCCCCAGCCTCAGTCCAACATCCTCTTCTCCATCAACAATGAGAGTGTGGCT GCAGTGAGCAGCGCCGGGCTGGTGCGTGGGCTCATGGTTGGCAATGGCTCTGTACTGGGAGTTGTGCAGG CAGTGGATGCCGAGACTGGCAAGGTCATCATCGTCTCCCAGGATCATGTGGAAGTAGAGGTGCTACAGCT CCAGGCAGTGAGGATCCGAGCCCCGATCACACGAATGAGGACCGGGACCCAGATGCCTGTCTATGTCACC GGGATCACCAGCAACCAGAGCCCTTTCTCCTTTGGCAATGCTGTGCCAGGCCTGACCTTCCACTGGTCTG TCACCAAGAGGGATGTCCTGGACCTTCGAGGACGGCACCATGAGGTGTCAATCCGGCTCTCGCCTCAGTA CAATTTTGCCATGAATGTGCATGGCCGGGTGAAAGGTCGGACTGGACTGCGGGTGGTGGTCAAAGCTCTG GACCCCACAGCTGGGCAGCTGCATGGTCTCGGCAAGGAGCTCTCAGATGAGATCCAAATTCAGGTATTTG AAAAACTGCGGCTGCTGAACCCTGAAGTAGAAGCAGAACAAATACTAATGTCACCTAACTCGTTTATAAA GCTGCAGACAAACAGGGATGGTGCAGCCATTCTGAGCTACCGGGTCCTCGATGGGCCTGAGAAGGCTCCC ATCGTGCACATTGACGAGAAGGGCTTTCTGGTGTCCGGCTCTGGGATTGGAGTGTCCACCCTCGAAGTGA TTGCACAGGAGCCTTTCGGCACCAACCAGACTGTCCTTGTCGCTGTAAAGGTCTCTCCTATTTCATACCT GAGGATCTCCATGAGCCCAGTCCTGCACACTCAGCACAAGGAGGTGCTGACAGCTCTGCCTCTGGGGATG ACTGTGACCTTCACTGTCCACTTCCATGACAGCTCTGGAGACATCTTCCACGCTCATAATTCAGACCTCA ATTTCGCTACTAACAGAGATGACTTCGTGCAGATTGGCAAGGGGGCCACCAACAACACCTGCATCATCCG CACAGTCAGTGTGGGCTTGACACTGCTCCACGTGTGGGATGTGGAACATCTCGGCCTCTCCGACTTCGTG CCACTACCTGTCCTGCAGGCCATCACCCCAGAGTTGTCTGGAGCTGTGGTGGTGGGCGACATCCTCTGCC TGGCTAGCGTTCTCATTAGCCTGGGAGGTGTCTCAGGGACCTGGAGCTCCTCAGCTGGCAATGTCCTCTA TGTTGACCCCAAGACAGGCGTGGCTATAGCCCGAGATGCAGGGCCTGTGACTGTTTACTATGAAATCGCT GGACATCTGAAGACCTTCAAGGAGATAGTGGTCGTCACCCCTCAGAAGATTGTGGCCCGTCGCCTCCACG CCACGCAGACCAGTATCCAGGAAGCCACAGCCTCTAAAGTGACTGTCAGCGTGGGAGACAGGAGCTCTAA CCTACTTGGTGAGTGCTCCTCTGCCCAGCGAGAGGCCATAGAAGCCTTGCACCCAGAGTCCCTCATCAGC TGCCAACTCCAGTTCAAGCAAGATGTCTTTGACTTCCCTGCTCGTGACATCTTCTCTGTGGAACCAGGAT TTGATACTGCACTGGGCCAGTACCTCTGCTCGGTTACTATGCACAGACTGACAGATAAGCAACTGAAGCA CTTGAACATGAAGAAGACATCACTGGCGGTCACCGCCTCCATGCCTAGCAGCCGTACATCTGTAGAAAAG GTGGGAGCTGAGGTCCCCTTCAGTCCAGGGCTCTATGCCAACCAGGCAGAAATCCTTTTGAGCAACCACT ACCCCAGCTCCGAGGTCAAGATCTTCGGTGCTGTGGAAATTCTGGAAAACTTGGAGGTGAGGTCCGGGTC CCCAGCAGTGCTAGCCTCCGTGAAAGAGAAGTCATTTGGACTGCCCAGCTTCATCACGTACACAGTTGGT GTCTTGGATCCCACAGCTGGCAGCCAAGGCCCTCTGTCCACTGCCCTGACCTTCTCCAGTCCTGCCACTA ACCAGGCCATCACCATTCCAGTCACTGTGGCCTTTGTGTTGGATCGTCGTGGGCCTGGTCCCTATGGAGC CAGCCTCCTGTCACACTTCCTGGACTCCTATCAAGTCATGTTCTTCACCTTTTTTGCCCTGCTGGCTGGG ACAGCAGTCACCATTATAGCCTACCACACGGTCTGTGCACCCCGGGAACTTGCTTCACCACTGGCCCTCA CGCCCCATGCCAGCCCTCAGCACAGCCCCCACTATCTTGCCTCATCCCCGACTGCTTTCAACACACTGCC TTCTGATCGCAAAGCCAGCCCTCCCTCAGGGCTCTGGAGCCCAGCCTATGCCTCTCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR207371 representing NM_053322 Red=Cloning site Green=Tags(s) MARASLIQPGLWALLLLQAVGPAVAAKLNIPKVLLPFTRATRVNFTLEASEGCYRWSSTRPEVASIEPLG SSEQQCSQKAVVQARLTQPARLTSIIFAEDITTGQVLRCDAIVDLIHGIQIVSTTRELYLEDSPLELKIQ ALDSEGNTFSTLAGLVFDWTIVKDTEANGFSDSHNALRILTFLESTYIPPSYISEMEKAAKQGDTILVSG MKTGSSKLKARIQEAVYKNVRPAEVRLLILENILLNPAYDVYLLVGTSIHYKVQKIRQGKITELSMPSDQ YELQLQNSIPDPQGDPARPVAVLTQDTSRVTAMQMGQSNLVLGHRSIRMQGASRLPNSTIYVVEAGYLGF TVHPGDRWVLETGHLYAVTIEVFDRSSNKVYPSDNIRIEAVFPAEFFEVLSSSQNGSYHHVRAIQSGQTT ISASLTSVVDQDGGVHVLQVPVWNQQEVDIHIPITLYPSILTFPWQPKTGAYQYTIKAHGGSGNFTWSSS SYMVATVTVKGVMTTGGDTGLSVIRAHDVQNPLHFGEMKVYVIEPSSMEFAPCQVEARVGHTLELPLTIS GLMPGGSSEVVTLSDCSHFDLVVEVENQGVFQPLPGRLPPGPEHCSGVKVRADAQGSTTLLVSYTHGHVH LGAKITLAAYLPLKAVDPSSVAVVTLGSSKEMLFEGGPRPWVLEPSKFFRNVTSEDTGSISLSLLGPPAS RNYQQHRVLVTCQALGEQVIALSVGNRPSLSNPFPAVEPTVVKSVCAPPSRLTLMPVYALPQLDLSCPLL QQNKQVVPVSSHRNPLLDLGAYDQQGRRFDNFSSLSIQWESFRPLLASIEVDQPMQLVSQDDGNGQKKLH GLQTVSVHEASGTTAISATATGYQQSHLSAAGVKQLRDPLVPVSASIELILVEDVRVSPEEVTIYNHPGV QVELHITEGSGYFFLNTSTQDIINVAYQDTRGVAMVHPLFPGSSTVMVHDLCLTFPAPAKATIHVSDIQE LYVRVVDKVEIGKAVKAYVRVLDFYKKPFLAKYFTFMDLKLRAASQIITLVTLDEALDNYTATFLVHGVA IGQTSLSASVTDKSGQRVSSTAQQIEVFPPFRLIPRKVTLIIGAMIQITSEGGPQPQSNILFSINNESVA AVSSAGLVRGLMVGNGSVLGVVQAVDAETGKVIIVSQDHVEVEVLQLQAVRIRAPITRMRTGTQMPVYVT GITSNQSPFSFGNAVPGLTFHWSVTKRDVLDLRGRHHEVSIRLSPQYNFAMNVHGRVKGRTGLRVVVKAL DPTAGQLHGLGKELSDEIQIQVFEKLRLLNPEVEAEQILMSPNSFIKLQTNRDGAAILSYRVLDGPEKAP IVHIDEKGFLVSGSGIGVSTLEVIAQEPFGTNQTVLVAVKVSPISYLRISMSPVLHTQHKEVLTALPLGM TVTFTVHFHDSSGDIFHAHNSDLNFATNRDDFVQIGKGATNNTCIIRTVSVGLTLLHVWDVEHLGLSDFV PLPVLQAITPELSGAVVVGDILCLASVLISLGGVSGTWSSSAGNVLYVDPKTGVAIARDAGPVTVYYEIA GHLKTFKEIVVVTPQKIVARRLHATQTSIQEATASKVTVSVGDRSSNLLGECSSAQREAIEALHPESLIS CQLQFKQDVFDFPARDIFSVEPGFDTALGQYLCSVTMHRLTDKQLKHLNMKKTSLAVTASMPSSRTSVEK VGAEVPFSPGLYANQAEILLSNHYPSSEVKIFGAVEILENLEVRSGSPAVLASVKEKSFGLPSFITYTVG VLDPTAGSQGPLSTALTFSSPATNQAITIPVTVAFVLDRRGPGPYGASLLSHFLDSYQVMFFTFFALLAG TAVTIIAYHTVCAPRELASPLALTPHASPQHSPHYLASSPTAFNTLPSDRKASPPSGLWSPAYASH TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_053322
ORF Size	5658 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_053322.2, NP_445774.1
RefSeq Size	6941 bp
RefSeq ORF	5661 bp
Locus ID	58958
UniProt ID	P11654
MW	204.2 kDa
Gene Summary	major component of the nuclear pore complex; may play a role in anchoring the central framework of the pore complex to the membrane [RGD, Feb 2006]

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