Cd320 (NM_001014201) Rat Tagged ORF Clone

CAT#: RR207203

  • TrueORF®

Cd320 (Myc-DDK-tagged ORF) - Rat CD320 molecule (Cd320), (10 ug)



  "NM_001014201" in other vectors (3)

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CNY 3,990.00


货期*
5周

规格
    • 10 ug

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经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Synonyms RGD1305860
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR207203 representing NM_001014201
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCGGTGCGGAGCTGGGCGGGCAGCCGCCCTGGGCCTGGTGCTGCGGCTGCTCTTGGGTCTTAGAA
CAGGTCCCGAGGCCGCTCCGGCTCCAACCTCGGCTCCGGCCCATACCCTAGTCCAAGTCTCGGGCCCTAG
AGCTGGCTCCTGCCCAACAGACACCTTCAAGTGTCTTACCAGTGGCTACTGTGTGCCCCTCTCCTGGCGC
TGCGATGGGGACCGGGATTGCTCTGATGGCAGTGACGAGGAAGAGTGTAGAATTGAGCCATGTGCTCAGA
ATAGGCAGTGCCAGCCACAGCCTGCCCTTCCTTGCTCCTGTGACAACATCAGTGGTTGCTCCGCTGGCTC
AGACAAGAACCTCAACTGTAGCCGCTCACCCTGTCAGGAGGGCGAGCTGCGTTGCATACTGGATGATGTC
TGTATCCCACACACGTGGCGCTGTGATGGCCACCCAGACTGTCCTGACTCCAGTGATGAGCTCAGCTGTG
ACACCGACACAGAAACTGACAAGATATTCCAGGAGGAGAATGCCACAACCTCAATGTCTTCTATGATTGT
GGAGAAAGAGACCTCTTTCAGAAATGTCACAGTTGCTTCTGCTGGGCACCCATCCCGAAACCCAAATGCC
TATGGGGTTATCGCAGCTGCTGGGGTGTTGAGTGCCATCCTGGTTTCAGCTACCATTCTCATATTGCTAC
GACTCAGAGGTCAGGGTTATCTACCCCCAACAGGGCTGTTGGTGGCTGTGAAGGAGTCGCTGCTGCTGTC
AGAAAGGAAAACCTCTTTGATC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR207203 representing NM_001014201
Red=Cloning site Green=Tags(s)

MARCGAGRAAALGLVLRLLLGLRTGPEAAPAPTSAPAHTLVQVSGPRAGSCPTDTFKCLTSGYCVPLSWR
CDGDRDCSDGSDEEECRIEPCAQNRQCQPQPALPCSCDNISGCSAGSDKNLNCSRSPCQEGELRCILDDV
CIPHTWRCDGHPDCPDSSDELSCDTDTETDKIFQEENATTSMSSMIVEKETSFRNVTVASAGHPSRNPNA
YGVIAAAGVLSAILVSATILILLRLRGQGYLPPTGLLVAVKESLLLSERKTSLI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001014201
ORF Size 792 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001014201.1, NP_001014223.1
RefSeq Size 1192 bp
RefSeq ORF 795 bp
Locus ID 362851
UniProt ID Q5HZW5
MW 27.9 kDa
Gene Summary Receptor for transcobalamin saturated with cobalamin (TCbl). Plays an important role in cobalamin uptake. Plasma membrane protein that is expressed on follicular dendritic cells (FDC) and mediates interaction with germinal center B cells. Functions as costimulator to promote B cell responses to antigenic stimuli; promotes B cell differentiation and proliferation. Germinal center-B (GC-B) cells differentiate into memory B-cells and plasma cells (PC) through interaction with T-cells and follicular dendritic cells (FDC). CD320 augments the proliferation of PC precursors generated by IL-10.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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