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Mep1a (NM_013143) Rat Tagged ORF Clone
CAT＃: RR207167
- TrueORF®
Mep1a (Myc-DDK-tagged ORF) - Rat meprin 1 alpha (Mep1a), (10 ug)
"NM_013143" in other vectors (3)
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CNY 9,690.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR207167 representing NM_013143
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGTGGACACTACCTGTGTGTCTTCTCTCACTGAGTTTTTCTGCCCACATTGCAGCTGTGTCCATTC AGCATCTTTCGACTGGACATGACCACGATGATGTAGACGTTGGTGAACAACAGAAGGACATTTCAGAAAT CAACTCAGCTGCAGGCCTGAACCTTTTTCAAGGGGACATCCTTCTGCCCAGAACCAGAAATGCCCTGAGA GATCCGTCAAGCAGATGGAAGCCCCCCATTCCATACATTCTGGCTGACAATCTGGATCTGAACGCCAAAG GAGCCATCCTCAATGCCTTTGAGATGTTCCGCCTCAAGTCTTGTGTGGACTTCAAGCCCTATGAGGGGGA GAGCTCGTACATCATCTTCCAGCAGTTTTCTGGGTGCTGGTCTATGGTCGGTGATCAACACGTGGGACAG AATATTTCCATTGGTGAGGGCTGCGACTATAAGGCCATCATTGAACATGAGATCCTGCATGCTCTGGGAT TCTTCCATGAGCAGTCAAGGACTGACCGGGATGATTATGTGAACATCTGGTGGAATGAAATTATGACAGA TTATGAACATAATTTCAACACGTATGATGACAAGACCATCACAGACCTCAACACACCCTATGATTATGAG TCCTTGATGCACTATGGGCCATTTTCATTTAACAAGAATGAAACCATCCCGACCATCACTACCAAGATCC CTGAGTTCAACGCCATCATTGGACAGCGCCTGGATTTCAGTGCCACTGATTTAACGAGACTGAATCGGAT GTACAACTGCACCAGAACACATACCCTGCTGGACCACTGTGCGTTTGAGAAGACCAACATCTGTGGAATG ATCCAGGGGACCAGAGATGATGCTGACTGGGTTCATGAGGACAGCAGTCAGCCTGGACAAGTGGATCACA CCTTGGTGGGACGGTGCAAAGCTGCTGGCTACTTCATGTACTTCAATACCAGCTCGGGGGTGACCGGAGA GGTAGCCCTTCTGGAGTCTCGGATCCTTTACCCCAAGAGGAAGCAGCAGTGTTTGCAGTTTTTTTATAAG ATGACAGGAAGCCCTTCAGACAGACTCCTCATCTGGGTGAGAAGGGACGACAACACGGGCAACGTGCGCC AGCTGGCCAAGATCCAGACTTTTCAAGGAGATTCTGATCACAACTGGAAAATTGCTCACGTGACACTCAA CGAAGAGAAGAAATTTCGCTATGTTTTCCAGGGTACCAAAGGTGACCCTGGTAACTCAGATGGCGGTATT TACCTTGATGACATCACTCTGACAGAAACCCCCTGCCCTACAGGGGTTTGGACCATCCGGAATATCTCCC AGGTCCTTGAGAACACAGTGAAAGGGGACAGGCTCGTGAGCCCTAGGTTCTACAATTCCGAGGGTTATGG TTTTGGGGTGACCTTGTACCCCAATGGTAGAATCACCAGTAACTCTGGCTACCTGGGACTCGCTTTCCAC TTGTACAGTGGGGATAACGATGTGATTCTGGAGTGGCCAGTGGAGAACGAGCAGGCCATAATGACCATCC TGGACCAGGAACCTGATGCCAGGAACAGGATGTCCTTGAGTTTGATGTTCACTACCTCCAAGTACCAAAC ATCTTCAGCTATAAATGGCTCTGTCATCTGGGACAGGCCAACCAAGGTTGGAGTCTATGATAAGGACTGT GATTGTTTTAGAAGCATTGACTGGGGTTGGGGTCAGGCCATTTCCCACCAGATGCTGATGAGAAGAAATT TCCTTAAAGATGACACCCTCATCATCTTCGTGGACTTTAAAGACCTTACCCACTTGAGGCAGACTGAAGT CCCCATTTCCAGCAGAAGCGTGATACCCCGAGGACTCCTTCTGCAAGGCCAGGAGCCTCTTGCCTTGGGA GACTCCAGAATAGCCATGATGGAGGAATCCCTGCCAAGAAGGCTGGACCAGAGACAGCCCAGCAGACCAA AGCGCTCAGTGGAAAATACTGGTCCCATGGAGGACCACAACTGGCCACAGTACTTCAGAGACCCATGTGA CCCAAACCCTTGCCAGAATGAAGGCACCTGTGTGAACGTAAAGGGAATGGCCAGCTGCAGGTGCGTCTCT GGGCATGCCTTTTTCTACACAGGGGAGCGCTGTCAGGCTATGCATGTGCACGGAAGCTTGCTGGGCCTGC TCATTGGCTGCATCACTGCTTTGATCTTCTTGACCTTCATCACATTCTCCAACACTTACCAAAAGCTGAG GCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR207167 representing NM_013143
Red=Cloning site Green=Tags(s) MLWTLPVCLLSLSFSAHIAAVSIQHLSTGHDHDDVDVGEQQKDISEINSAAGLNLFQGDILLPRTRNALR DPSSRWKPPIPYILADNLDLNAKGAILNAFEMFRLKSCVDFKPYEGESSYIIFQQFSGCWSMVGDQHVGQ NISIGEGCDYKAIIEHEILHALGFFHEQSRTDRDDYVNIWWNEIMTDYEHNFNTYDDKTITDLNTPYDYE SLMHYGPFSFNKNETIPTITTKIPEFNAIIGQRLDFSATDLTRLNRMYNCTRTHTLLDHCAFEKTNICGM IQGTRDDADWVHEDSSQPGQVDHTLVGRCKAAGYFMYFNTSSGVTGEVALLESRILYPKRKQQCLQFFYK MTGSPSDRLLIWVRRDDNTGNVRQLAKIQTFQGDSDHNWKIAHVTLNEEKKFRYVFQGTKGDPGNSDGGI YLDDITLTETPCPTGVWTIRNISQVLENTVKGDRLVSPRFYNSEGYGFGVTLYPNGRITSNSGYLGLAFH LYSGDNDVILEWPVENEQAIMTILDQEPDARNRMSLSLMFTTSKYQTSSAINGSVIWDRPTKVGVYDKDC DCFRSIDWGWGQAISHQMLMRRNFLKDDTLIIFVDFKDLTHLRQTEVPISSRSVIPRGLLLQGQEPLALG DSRIAMMEESLPRRLDQRQPSRPKRSVENTGPMEDHNWPQYFRDPCDPNPCQNEGTCVNVKGMASCRCVS GHAFFYTGERCQAMHVHGSLLGLLIGCITALIFLTFITFSNTYQKLRQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013143 |
| ORF Size | 2244 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013143.1, NP_037275.1 |
| RefSeq Size | 2928 bp |
| RefSeq ORF | 2247 bp |
| Locus ID | 25684 |
| UniProt ID | Q64230 |
| MW | 85.1 kDa |
| Gene Summary | alpha subunit of meprin metalloproteases, which form homotetramers and heterodimers with beta subunit Mep1b [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN207167 | Mep1a (untagged ORF) - Rat meprin 1 alpha (Mep1a), (10 ug) |
CNY 9,690.00 |
|
| RR207167L3 | Lenti ORF clone of Mep1a (Myc-DDK-tagged ORF) - Rat meprin 1 alpha (Mep1a), (10 ug) |
CNY 11,500.00 |
|
| RR207167L4 | Lenti ORF clone of Mep1a (mGFP-tagged ORF) - Rat meprin 1 alpha (Mep1a), (10 ug) |
CNY 12,540.00 |
