Sema3d (NM_001104633) Rat Tagged ORF Clone
CAT#: RR207123
- TrueORF®
Sema3d (Myc-DDK-tagged ORF) - Rat sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (Sema3d), (10 ug)
"NM_001104633" in other vectors (3)
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CNY 9,980.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR207123 representing NM_001104633
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATGCTAATAAAGATGAGAACCCAAGGCCCAGAAGTCAAGATCTTCACCTTTTTCATGCTGGGATGA TGTTAATCGTGACTGTGCTATATCTTCCTGTCACTGAAACATCTAAACAAAATATTCCACGGCTCAAGCT AACCTACAAAGACTTGCTGCTCTCAAACACCTGCATTCCCTTTTTGGGTTCATCAGAAGGACTGGATTTC CAGACTCTTCTTTTGGATGAGGAGAGGGGCATACTGCTCCTAGGAGCCAAAGACCATGTCTTCCTGCTCA ATCTGGTAGACTTGAACAAGAACTTTAAGAAGATTTATTGGCCTGCTGCAAAGGAACGAGTGGAACTATG TAAACTAGCTGGGAAAGATGCCAATACAGAATGTGCAAATTTCATCCGTGTGCTTCAACCCTACAATAAG ACTCATGTTTACGTGTGTGGAACTGGAGCATTTCATCCACTGTGTGGATATATTGATCTTGGAGCCAACA AGGAGGAACTCATATTTAAACTAGACATGCAGAACCTGGAGTCTGGCAGACTGAAATGTCCCTTTGATCC TCAGCAGCCTTTTGCTTCGGTAATGACAGATGAGCACCTCTACTCTGGAACAGCTTCTGATTTTCTTGGC AAAGACACTGCATTCACAAGATCCCTTGGGCTAATGCAGGACCACCATTACATCAGAACTGACATTTCGG AACACTACTGGCTCAATGGAGCAAAATTTATCGGAACGTTCCCCATCCCAGACACCTATAATCCGGACGA TGACAAAATATATTTCTTCTTTCGGGAATCATCTCAGGAAGGCAGTACTTCTGACAGAAGCATTCTTTCA AGAGTTGGGAGAGTTTGTAAGAATGATGTGGGTGGGCAGCGGAGTCTGATAAACAAATGGACTACTTTTC TTAAGGCAAGACTGGTTTGCTCAATTCCTGGAAGTGATGGGGCAGATACTCACTTTGATGAACTTCAAGA TATTTACTTACTCCCTACGAGAGATGAAAGAAATCCTGTTGTGTATGGAGTCTTTACCACAACCAGTTCC ATCTTCAAAGGCTCTGCTGTCTGTGTGTACAGCATGGCTGATATCAGAGCAGTTTTTAACGGTCCCTATG CTCATAAGGAAAGTGCAGACCATCGTTGGGTGCAATATGATGGAAGGATACCTTATCCCCGACCCGGAAC GTGTCCCAGCAAAACCTATGATCCACTGATTAAGTCCACCCGAGACTTCCCAGATGATGTTATCAGTTTC ATAAGGCGGCACCCGGTGATGTATAAGTCGGTGTATCCAGTGGCCGGAGCACCCACCTTCAAGAGAATTA ACGTGGATTACAGACTGACGCAGATAGTGGTGGATCACGTGGTCGCTGAAGACGGACAGTATGATGTCAT GTTTCTCGGAACAGACATTGGAACCGTCCTGAAAGTTGTGAGCATTTCCAAGGAGAAGTGGAATATGGAG GAGGTAGTCCTCGAGGAGCTTCAGGTATTCAAGCACCCAACAGCCATCTTGAACATGGAGTTGTCGCTGA AGCAGCAACAGTTGTACGTCGGTTCCTGGGATGGATTGGTTCAGCTCTCCTTGCACAGATGCGATACTTA TGGGAAAGCCTGTGCAGACTGCTGTCTCGCCAGAGACCCTTACTGTGCTTGGGATGGAAATGCTTGCTCC AGATATGCACCCACTTCCAAAAGGCGAGCTAGACGCCAGGATGTAAAATATGGAGACCCAATCACTCAGT GCTGGGACATAGAAGACAGCATTAGTCATGAAGCAGCTGATGAAAAGGTGATTTTCGGAATTGAATTTAA TTCAACCTTTTTGGAATGTATACCTAAATCCCAACAAGCCTCTGTTGAGTGGTACATCCAGCGGTCAGGA GATGAGCATCGAGAAGAGTTGAAACCCGATGAAAGGATCATCAAAACGGACTATGGGCTACTGATTCGAA GCCTGCAGAAGAAGGACTCTGGGATTTATTACTGCAAAGCACAGGAGCACACTTTCATCCACACCATAGT GAAGCTGACTTTGAATGTCATTGAGAATGAACAGATGGAAAATAACCAGAGGGCAGAATACCAGGAGGGG CAGGTCAAGGATCTGTTGGCTGAGTCACGGTTGAGATACAAAGACTACATCCAAATCCTTAGCAGCCCAA ACTTCAGCCTGGACCAGTACTGTGAGCAGATGTGGTACAGGGAGAAGCGGAGACAGCGCAACAAGGGCAG CGCAAAGTGGAAGCACATGCAGGAAATGAAGAAGAAACGGAATCGACGGCATCACAGAGACCTGGATGAG CTTCAGAGATCAGTGGCTACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR207123 representing NM_001104633
Red=Cloning site Green=Tags(s) MNANKDENPRPRSQDLHLFHAGMMLIVTVLYLPVTETSKQNIPRLKLTYKDLLLSNTCIPFLGSSEGLDF QTLLLDEERGILLLGAKDHVFLLNLVDLNKNFKKIYWPAAKERVELCKLAGKDANTECANFIRVLQPYNK THVYVCGTGAFHPLCGYIDLGANKEELIFKLDMQNLESGRLKCPFDPQQPFASVMTDEHLYSGTASDFLG KDTAFTRSLGLMQDHHYIRTDISEHYWLNGAKFIGTFPIPDTYNPDDDKIYFFFRESSQEGSTSDRSILS RVGRVCKNDVGGQRSLINKWTTFLKARLVCSIPGSDGADTHFDELQDIYLLPTRDERNPVVYGVFTTTSS IFKGSAVCVYSMADIRAVFNGPYAHKESADHRWVQYDGRIPYPRPGTCPSKTYDPLIKSTRDFPDDVISF IRRHPVMYKSVYPVAGAPTFKRINVDYRLTQIVVDHVVAEDGQYDVMFLGTDIGTVLKVVSISKEKWNME EVVLEELQVFKHPTAILNMELSLKQQQLYVGSWDGLVQLSLHRCDTYGKACADCCLARDPYCAWDGNACS RYAPTSKRRARRQDVKYGDPITQCWDIEDSISHEAADEKVIFGIEFNSTFLECIPKSQQASVEWYIQRSG DEHREELKPDERIIKTDYGLLIRSLQKKDSGIYYCKAQEHTFIHTIVKLTLNVIENEQMENNQRAEYQEG QVKDLLAESRLRYKDYIQILSSPNFSLDQYCEQMWYREKRRQRNKGSAKWKHMQEMKKKRNRRHHRDLDE LQRSVAT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001104633 |
ORF Size | 2331 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001104633.1, NP_001098103.1 |
RefSeq Size | 4922 bp |
RefSeq ORF | 2334 bp |
Locus ID | 246262 |
MW | 89.5 kDa |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN207123 | Sema3d (untagged ORF) - Rat sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (Sema3d), (10 ug) |
CNY 9,980.00 |
|
RR207123L3 | Lenti ORF clone of Sema3d (Myc-DDK-tagged ORF) - Rat sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (Sema3d), (10 ug) |
CNY 11,780.00 |
|
RR207123L4 | Lenti ORF clone of Sema3d (mGFP-tagged ORF) - Rat sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (Sema3d), (10 ug) |
CNY 12,920.00 |