Sema3f (NM_001108185) Rat Tagged ORF Clone

CAT＃: RR207003

  • TrueORF®

Sema3f (Myc-DDK-tagged ORF) - Rat sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3 F (Sema3f), (10 ug)

Datasheet
SDS
Plasmid Map

  "NM_001108185" in other vectors (3)

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CNY 6,328.00

货期*
现货
规格
    • 10 ug

Product images

经常一起买 (4)
PS100001
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00

添加到购物车
CC100003
DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00

暂停销售
PRIMER
Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

添加到购物车
TA592569S
DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

添加到购物车

【特别福利】购一抗正装产品，免费领10ul抗体试用装

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Synonyms SemaIV
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR207003 representing NM_001108185
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTTGTCACTGGCTTCATCCTCTGGGCTTCCCTGCTGACTGGGGCCTGGCCAGCTGCCCCCATCCAGG
ACCAGCTTCCAGCCACACCCAGAGTCCGACTATCTTTCAAAGAACTTAAGGCCACAGGTACTGCCCACTT
CTTCAACTTTCTGCTCAACACTACAGACTACAGAATCCTGTTCAAGGACGAGGACCATGACCGCATGTAT
GTGGGCAGCAAGGACTACGTGCTGTCCCTGGACCTGCATGACATCAACCGAGAGCCCCTTATTATCCACT
GGGCAGCCTCCCCACAGCGCATTGAGGAGTGCATATTGTCAGGCAAGGATGGCAACGGAGAGTGTGGGAA
CTTCGTCAGACTCATCCAGCCCTGGAACCGGACACACCTGTACGTGTGCGGGACCGGTGCCTACAACCCC
ATGTGCACCTATGTGAACCGTGGCCGTCGCTCACAGGCCCCACCGTGGACCCAGATGCAGGTGGTCAGAG
GCCGAGGCAGCAGGGCCACAGATGGTGCCGACCGCCCGACGCCCACAGCCCCACGCCAGGATTACATCTT
CTACCTGGAGCCTGAGAAACTTGAGTCAGGGAAAGGCAAATGCCCATACGACCCCAAGCTGGACACGGCC
TCAGCCCTCATCAATGAGGAGCTCTACGCAGGAGTGTACATCGATTTTATGGGCACTGATGCAGCCATTT
TCCGTACGCTTGGAAAGCAGACAGCCATGCGCACAGATCAGTACAACTCCCGGTGGCTCAATGATCCTTC
ATTCATACATGCTGAGCTCATCCCTGACAGCGCTGAGCGCAACGACGATAAACTCTACTTCTTCTTCCGA
GAGCGCTCTGCAGAGGCTCCGCAGAACCCTGCGGTGTACGCCCGCATCGGGCGCATCTGCCTCAACGATG
ACGGTGGCCATTGCTGCCTGGTCAACAAGTGGAGCACATTCCTGAAGGCACGGCTCGTCTGCTCTGTGCC
AGGCGAGGATGGCATTGAGACCCACTTCGATGAACTCCAGGACGTGTTTGTCCAGCAGACCCAGGACGTC
AGGAACCCAGTCATTTATGCTGTCTTCACCTCTTCAGGCTCTGTGTTCCGAGGATCTGCAGTGTGTGTCT
ACTCTATGGCTGACATCCGCATGGTCTTCAATGGGCCTTTTGCTCACAAGGAGGGCCCCAACTACCAGTG
GATGCCTTTCTCAGGAAAGATGCCGTATCCCCGGCCTGGCACATGCCCTGGTGGAACCTTTACGCCATCG
ATGAAGTCCACCAAGGACTATCCTGATGAAGTGATCAACTTCATGCGTACTCATCCACTCATGTACCAGG
CCGTCTACCCGCTGCAGCGGCGCCCCCTGGTGGTCCGTACAGGCGCTCCCTACCGCCTCACCACTGTCGC
TGTGGACCAGGTGGATGCAGCTGATGGACGCTATGAGGTGCTTTTCCTGGGCACAGACCGCGGGACAGTG
CAGAAGGTCATCGTGCTGCCCAAGGATGATCAGGAGGTGGAAGAGCTCATGTTAGAGGAGGTGGAGGTCT
TCAAGGAGCCAGCACCTGTTAAAACTATGACCATCTCTTCCAAGAGGCAACAACTGTATGTGGCCTCCGC
CGTGGGCGTCACACACCTGAGCCTGCACCGCTGTCAGGCCTATGGAGCTGCCTGTGCTGACTGCTGCCTC
GCCCGAGATCCCTACTGTGCCTGGGATGGCCAGGCCTGCTCTCGCTACACAGCATCCTCCAAGAGGCGGA
GTCGCCGACAAGATGTCCGCCACGGGAACCCCATCAGGCAGTGCCGTGGTTTCAATTCCAATGCAAACAA
GAATGCCATAGAGTCTGTGCAGTACGGCGTGGCCGGAAGTGCGGCTTTCCTTGAGTGCCAGCCTCGGTCA
CCTCAAGCCACTGTTAAGTGGCTTTTCCAGCGAGATCCCAGTGATCGGCGTCGTGAGATTCGCGCAGAGG
ACCGCTTCCTGCGCACAGAGCAGGGGCTGTTGCTTCGCGCCCTGCAGCTTGGTGACCGCGGCCTCTACTC
CTGCACGGCCACTGAGAACAACTTCAAGCACGTCGTCACGCGGGTACAGCTGCATGTACTGGGCCGGGAC
GCCGTCCATGCTGCCCTCTTCCCCCCACTGGCTGTGAGCGTCCCTCCACCTCCTGGCACAGGCCCTCCAA
CACCTCCTTACCAGGAGCTGGCCCAGCTCCTAGCCCAACCGGAAGTGGGCCTCATCCATCAGTACTGCCA
GGGTTACTGGCGCCATGTGCCCCCCAACCCCAGGGAGGCTCCAGGAGCTCTCAGGCCTCCTGAACTCCAG
GACCAGAAAAAGCCGAGGAACCGCCGCCACCATCCTCCGGACACA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
>RR207003 representing NM_001108185
Red=Cloning site Green=Tags(s)

MLVTGFILWASLLTGAWPAAPIQDQLPATPRVRLSFKELKATGTAHFFNFLLNTTDYRILFKDEDHDRMY
VGSKDYVLSLDLHDINREPLIIHWAASPQRIEECILSGKDGNGECGNFVRLIQPWNRTHLYVCGTGAYNP
MCTYVNRGRRSQAPPWTQMQVVRGRGSRATDGADRPTPTAPRQDYIFYLEPEKLESGKGKCPYDPKLDTA
SALINEELYAGVYIDFMGTDAAIFRTLGKQTAMRTDQYNSRWLNDPSFIHAELIPDSAERNDDKLYFFFR
ERSAEAPQNPAVYARIGRICLNDDGGHCCLVNKWSTFLKARLVCSVPGEDGIETHFDELQDVFVQQTQDV
RNPVIYAVFTSSGSVFRGSAVCVYSMADIRMVFNGPFAHKEGPNYQWMPFSGKMPYPRPGTCPGGTFTPS
MKSTKDYPDEVINFMRTHPLMYQAVYPLQRRPLVVRTGAPYRLTTVAVDQVDAADGRYEVLFLGTDRGTV
QKVIVLPKDDQEVEELMLEEVEVFKEPAPVKTMTISSKRQQLYVASAVGVTHLSLHRCQAYGAACADCCL
ARDPYCAWDGQACSRYTASSKRRSRRQDVRHGNPIRQCRGFNSNANKNAIESVQYGVAGSAAFLECQPRS
PQATVKWLFQRDPSDRRREIRAEDRFLRTEQGLLLRALQLGDRGLYSCTATENNFKHVVTRVQLHVLGRD
AVHAALFPPLAVSVPPPPGTGPPTPPYQELAQLLAQPEVGLIHQYCQGYWRHVPPNPREAPGALRPPELQ
DQKKPRNRRHHPPDT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001108185
ORF Size 2355 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001108185.1, NP_001101655.1
RefSeq Size 3485 bp
RefSeq ORF 2358 bp
Locus ID 315996
MW 88.5 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

SKU Description Size Price
RN207003 Sema3f (untagged ORF) - Rat sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3 F (Sema3f), (10 ug)
    • 10 ug

CNY 10,070.00

RR207003L3 Lenti ORF clone of Sema3f (Myc-DDK-tagged ORF) - Rat sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3 F (Sema3f), (10 ug)
    • 10 ug

CNY 11,880.00

RR207003L4 Lenti ORF clone of Sema3f (mGFP-tagged ORF) - Rat sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3 F (Sema3f), (10 ug)
    • 10 ug

CNY 8,728.00

You may also need
plasmid icon Empty Vector
protein iconDDK/Flag Antibody

其它Sema3f产品

Expression Plasmids
Lentiviral Particles
CRISPR
RNAi
Proteins
Antibodies
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RR207003L4V

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RR207003L4

Lenti ORF clone of Sema3f (mGFP-tagged ORF) - Rat sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3 F (Sema3f), (10 ug)

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CNY 8,728.00

RR207003L3

Lenti ORF clone of Sema3f (Myc-DDK-tagged ORF) - Rat sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3 F (Sema3f), (10 ug)

    • 10 ug

CNY 11,880.00

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