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Adam15 (NM_020308) Rat Tagged ORF Clone
CAT＃: RR206676
- TrueORF®
Adam15 (Myc-DDK-tagged ORF) - Rat a disintegrin and metallopeptidase domain 15 (metargidin) (Adam15), (10 ug)
"NM_020308" in other vectors (3)
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CNY 10,450.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MDC15; tMDCVI |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR206676 representing NM_020308
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGCTGGCGCTGCTCTGGGCCCTGGGACTCCTGGGCGCGGGCAGCCCTCGGCCCTCCCCGCCGCTGC CAAATATAGGAGGTACTGAGGAAGAGCAGCAAGCCAGCCCAGAGAGGACCCAGAGTAGATCCTTGGAGAA CCAGGTTGTTCAGGACAGCCCCCCAATTAACCTAACAGAAGTGCTTCAGACTGGTTTACCTGAGACCCTG AGGATTGGATTGGAGCTGGACGGTGAGAATCATATCCTGGAGCTTCAACAGAATAGAGATCTGGTCCCTG GCCGCCCGACTCTGGTGTGGTATCAGCCTGACGGCACCCGAATGGTCAGCGAGGGGCACAGTCTGGAAAA CTGCTGCTATCGAGGACGAGTTCAGGGCCGCCCCAGCTCTTGGGTCTCCCTCTGTGCCTGCTCTGGGATC AGGGGGCTGGTAGTCCTGTCCCCTGAGAGAAGCTATACACTGGAGCTGGGACCTGGAGACCTTCAGCGTC CTCTCATTGTTTCTCGGATCCAAGACCTCCTCTTGCCAGGCCACACCTGTGCCCCAAGCTGGCATGCATT TGTGCCCACCGAGGCAGCACCAGACCTCCTTTTGGAACAGCACCACCTTCGCAGGCTTAAGCGGGATGTA GTAACAGAGACGAAAATTGTCGAACTGGTGATTGTGGCTGATAATTCAGAGGTCAGGAAGTACCCTGACT TCCAACAACTGCTGAACCGAACACTAGAAGTGGCCCTTCTGCTAGACACATTCTTCCAGCCCCTGAATGT CCGGGTAGCACTTGTGGGCCTAGAGGCATGGACCCAGCGCGACCTGATAGAGATGAGCTCCAACCCAGCT GTCCTGCTAGACAACTTCCTCCGCTGGCGCCGGACAGACTTGCTGCCTCGACTGCCCCATGATAGTGCCC AGCTTGTGACTGTTACTTCCTTCTCTGGGCCCATGGTGGGCATGGCCATTCAGAATTCCATCTGTTCTCC TGACTTCTCGGGAGGTGTGAATATGGACCACTCGACAAGCATCTTAGGAGTTGCCTCCTCCATTGCCCAC GAGTTGGGTCACAGCCTGGGCCTAGACCACGATTCACCTGGGAACAGTTGTCCCTGTCCAGGTCCAGCCC CAGCCAAGAGCTGCATCATGGAGGCCTCCACAGATTTCCTACCAGGTCTGAACTTCAGCAACTGCAGCCG ATGGGCCCTGGAAAAAGCCCTCCTAGATGGGATGGGCAGCTGCCTCTTTGAATGGCCACCCAGCCGGGCC CCTATGTCCTCTTTGTGTGGAAATATGTTTGTGGACCCTGGAGAGCAGTGTGACTGTGGCTTCCCAGATG AGTGCACTGATCCTTGCTGTGACTATTTTACCTGCCAGCTGAGGCCGGGAGCACAGTGTGCATCTGACGG ACCCTGTTGTCAAAACTGCAAGTTACAGCCAGCTGGCTGGCAGTGCCGCCTTCCCACAGACGATTGCGAT TTGCCTGAGTTCTGCCTAGGAGATAGCTCTCAGTGCCCCCCTGACATCAGGCTTGGGGACGGTGAGCCTT GTGCTAGTGGAGAGGCTGTGTGTATGCATGGGCGCTGTGCCTCCTACACCCGGCAGTGCCAGTCGCTTTG GGGACCTGGGGCCCAGCCTGCTGCACCACTTTGCCTCCAAACAGCCAACACTCGGGGTAATGCCTTTGGG AGCTGTGGGCGCAGCCCCAGTGGTAGCTACATGCCTTGCAACCTTAGAGATGCCATTTGTGGGCAACTAC AGTGCCAGTGGGGTAGGAATCAGCCTCTGCTGGGCTCAGTCCAAGATCAGCTCTCGGAGGTCTTGGAAGC CAATGGGACACAATTAAACTGCAGCTGGGTAGATCTGGACTTGGGCAATGATGTGGCCCAGCCTCTCCTG GCTCTACCTGGCACTGCCTGTGGCCCTGGCCTGGTGTGCATCGGCCATCGATGCCAGCCTGTGGATCTCC TGGGAGCACAGGAATGCCGCAGCAAATGCCATGGCCATGGGGTCTGCGACAGCAGCAGGCACTGCCACTG TGACGAGGGCTGGGCACCTCCTGATTGCATGACCCAGCTCAGAGCAACCAGCTCCCTGACCACAGGCCTG CTCCTCAGCCTCCTGTTGTTATTGGTCCTCGTACTACTTGGTGCCAGCTACTGGTACCGTGCCCGCCTGC ATCAGCGGCTCTGCCAGCTCAAGGGATCCAGCTGCCAATATAGGGCAGCCCAATCCGGTCCTCCTGAGCG ACCAGGACCTCCACAGAGGGCACAGCAGATGCCAGGCACTAAGCCTCAGGGGCCTACCAAACCCCCACCC CCAAGAAAGCCACTGCCCGCCAACCCACAGGGCCGGCCCCCGCTGGGTGACCTGCCTGGCCCAGGAGATG GAAGCTTGCAGCTGGTAGTGCCTTCCAGGCCTGCCCCACCACCCCCAGCAGCATCTTCGCTCTACCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR206676 representing NM_020308
Red=Cloning site Green=Tags(s) MRLALLWALGLLGAGSPRPSPPLPNIGGTEEEQQASPERTQSRSLENQVVQDSPPINLTEVLQTGLPETL RIGLELDGENHILELQQNRDLVPGRPTLVWYQPDGTRMVSEGHSLENCCYRGRVQGRPSSWVSLCACSGI RGLVVLSPERSYTLELGPGDLQRPLIVSRIQDLLLPGHTCAPSWHAFVPTEAAPDLLLEQHHLRRLKRDV VTETKIVELVIVADNSEVRKYPDFQQLLNRTLEVALLLDTFFQPLNVRVALVGLEAWTQRDLIEMSSNPA VLLDNFLRWRRTDLLPRLPHDSAQLVTVTSFSGPMVGMAIQNSICSPDFSGGVNMDHSTSILGVASSIAH ELGHSLGLDHDSPGNSCPCPGPAPAKSCIMEASTDFLPGLNFSNCSRWALEKALLDGMGSCLFEWPPSRA PMSSLCGNMFVDPGEQCDCGFPDECTDPCCDYFTCQLRPGAQCASDGPCCQNCKLQPAGWQCRLPTDDCD LPEFCLGDSSQCPPDIRLGDGEPCASGEAVCMHGRCASYTRQCQSLWGPGAQPAAPLCLQTANTRGNAFG SCGRSPSGSYMPCNLRDAICGQLQCQWGRNQPLLGSVQDQLSEVLEANGTQLNCSWVDLDLGNDVAQPLL ALPGTACGPGLVCIGHRCQPVDLLGAQECRSKCHGHGVCDSSRHCHCDEGWAPPDCMTQLRATSSLTTGL LLSLLLLLVLVLLGASYWYRARLHQRLCQLKGSSCQYRAAQSGPPERPGPPQRAQQMPGTKPQGPTKPPP PRKPLPANPQGRPPLGDLPGPGDGSLQLVVPSRPAPPPPAASSLYL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020308 |
| ORF Size | 2448 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020308.1, NP_064704.1 |
| RefSeq Size | 2815 bp |
| RefSeq ORF | 2451 bp |
| Locus ID | 57025 |
| UniProt ID | Q9QYV0 |
| MW | 88.1 kDa |
| Gene Summary | Active metalloproteinase with gelatinolytic and collagenolytic activity. Plays a role in the wound healing process. Mediates both heterotypic intraepithelial cell/T-cell interactions and homotypic T-cell aggregation. Inhibits beta-1 integrin-mediated cell adhesion and migration of airway smooth muscle cells. Suppresses cell motility on or towards fibronectin possibly by driving alpha-v/beta-1 integrin (ITAGV-ITGB1) cell surface expression via ERK1/2 inactivation. Cleaves E-cadherin in response to growth factor deprivation. Plays a role in glomerular cell migration. Plays a role in pathological neovascularization. May play a role in cartilage remodeling. May be proteolytically processed, during sperm epididymal maturation and the acrosome reaction. May play a role in sperm-egg binding through its disintegrin domain (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN206676 | Adam15 (untagged ORF) - Rat a disintegrin and metallopeptidase domain 15 (metargidin) (Adam15), (10 ug) |
CNY 10,450.00 |
|
| RR206676L3 | Lenti ORF clone of Adam15 (Myc-DDK-tagged ORF) - Rat a disintegrin and metallopeptidase domain 15 (metargidin) (Adam15), (10 ug) |
CNY 12,260.00 |
|
| RR206676L4 | Lenti ORF clone of Adam15 (mGFP-tagged ORF) - Rat a disintegrin and metallopeptidase domain 15 (metargidin) (Adam15), (10 ug) |
CNY 13,490.00 |
