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Nagk (NM_001037768) Rat Tagged ORF Clone
CAT＃: RR206612
- TrueORF®
Nagk (Myc-DDK-tagged ORF) - Rat N-acetylglucosamine kinase (Nagk), (10 ug)
"NM_001037768" in other vectors (3)
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CNY 5,488.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MGC124788 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR206612 representing NM_001037768
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGCACTTTATGGTGGCGTGGAAGGGGGAGGCACACGGTCCAAAGTCCTTTTACTTTCTGAGGATG GGCAGATCCTGGCAGAAGCAGATGGACTGAGCACAAATCACTGGCTGATTGGCACAGGTACCTGTGTGGA GAGGATCAATGAGATGGTGGACAGGGCTAAACGGAAGGCTGGAGTGGATCCTCTGGTACCCCTTCGAAGC CTGGGCTTGTCCCTGAGTGGTGGGGAGCAGGAGGATGCAGTGAGGCTCCTGATGGAGGAGTTGAGGGACC GATTTCCCTACCTGAGTGAAAGTTACTTCATCACCACTGATGCAGCAGGTTCCATCGCCACAGCTACACC GGATGGTGGGATTGTGCTCATCTCTGGAACAGGCTCCAACTGTAGGCTTATCAACCCTGATGGCTCTGAG AGTGGCTGTGGTGGCTGGGGCCACATGATGGGAGACGAGGGATCAGCCTACTGGATTGCACACCAAGCTG TGAAAATTGTGTTTGACTCCATTGACAACCTGGAAGCAGCTCCTCATGATATTGGCCATGTCAAGCAGGC CATGTTCAACTACTTCCAGGTGCCAGATCGGCTAGGAATCCTCACTCACTTGTATAGGGACTTTGATAAG TCCAAGTTTGCTGGATTTTGTCAGAAAATTGCAGAAGGTGCACAGCAGGGAGACCCTCTTTCCAGGTTCA TCTTCAGAAAGGCTGGGGAGATGCTGGGCAGACACGTTGTGGCAGTATTGCCAGAGATTGACCCAGTTTT GTTCCAAGGGGAGCTTGGCCTCCCCATTCTGTGTGTGGGCTCAGTGTGGAAGAGCTGGGAGCTACTGAAG GAAGGCTTTCTCCTGGCACTGACGCAGGGCCGAGAGCAACAGGCACAGAACTCCTTCTCCAGTTTCACCC TGATGAAGTTGAGGCACTCTTCTGCACTGGGAGGGGCCAGCCTGGGGGCCAGGCACATTGGACACCACCT TCCCATGGACTACAGCGTCAATGCCATTGCCTTCTATTCCTATACCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR206612 representing NM_001037768
Red=Cloning site Green=Tags(s) MAALYGGVEGGGTRSKVLLLSEDGQILAEADGLSTNHWLIGTGTCVERINEMVDRAKRKAGVDPLVPLRS LGLSLSGGEQEDAVRLLMEELRDRFPYLSESYFITTDAAGSIATATPDGGIVLISGTGSNCRLINPDGSE SGCGGWGHMMGDEGSAYWIAHQAVKIVFDSIDNLEAAPHDIGHVKQAMFNYFQVPDRLGILTHLYRDFDK SKFAGFCQKIAEGAQQGDPLSRFIFRKAGEMLGRHVVAVLPEIDPVLFQGELGLPILCVGSVWKSWELLK EGFLLALTQGREQQAQNSFSSFTLMKLRHSSALGGASLGARHIGHHLPMDYSVNAIAFYSYTF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001037768 |
| ORF Size | 1029 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001037768.1, NP_001032857.1 |
| RefSeq Size | 1203 bp |
| RefSeq ORF | 1032 bp |
| Locus ID | 297393 |
| UniProt ID | P81799 |
| MW | 37.6 kDa |
| Gene Summary | Converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. Involved in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway. Also has ManNAc kinase activity.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
N-acetyl-D-glucosamine kinase binds dynein light chain roadblock 1 and promotes protein aggregate clearance
,Ripon, MKH;Lee, H;Dash, R;Choi, HJ;Oktaviani, DF;Moon, IS;Haque, MN;,
Cell Death Dis
,PubMed ID 32796833
[NAGK]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN206612 | Nagk (untagged ORF) - Rat N-acetylglucosamine kinase (Nagk), (10 ug) |
CNY 3,990.00 |
|
| RR206612L3 | Lenti ORF clone of Nagk (Myc-DDK-tagged ORF) - Rat N-acetylglucosamine kinase (Nagk), (10 ug) |
CNY 6,080.00 |
|
| RR206612L4 | Lenti ORF clone of Nagk (mGFP-tagged ORF) - Rat N-acetylglucosamine kinase (Nagk), (10 ug) |
CNY 6,650.00 |
