Sema4f (NM_019272) Rat Tagged ORF Clone

CAT＃: RR205977

  • TrueORF®

Sema4f (Myc-DDK-tagged ORF) - Rat sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4F (Sema4f), (10 ug)

Datasheet
SDS
Plasmid Map

  "NM_019272" in other vectors (3)

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CNY 9,980.00

货期*
5周
规格
    • 10 ug

Product images

经常一起买 (4)
PS100001
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00

添加到购物车
CC100003
DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00

暂停销售
PRIMER
Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

添加到购物车
TA592569S
DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

添加到购物车

【特别福利】购一抗正装产品，免费领10ul抗体试用装

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR205977 representing NM_019272
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTTGCCAGGGCCGAGCGGCCCCGCCCGGGCCCCCGGCCGCCTCCGGTCTTTCCCTTCCCGCCGCCGC
TGTCGCTGCTGCTGCTGCTGGCGATACTAAGCGCCCCGGTGTGCGGCCGCGTCCCCCGCTCAGTGCCCAG
AACCTCGCTGCCCATCTCCGAGGCTGACTCCTATCTCACCCGGTTTGCAGCGTCTCATACGTACAATTAC
TCTGCTCTCCTTGTGGATCCTGCCTCCCACACACTTTACGTCGGTGCACGGGATAGCATCTTCGCTTTAA
CCCTCCCCTTCTCTGGGGAAAGACCCCGAAGGATCGACTGGATGGTACCTGAGACTCACAGACAGAACTG
CAGGAAGAAAGGCAAGAAAGAGGACGAATGTCACAATTTTATCCAGATTCTCGCCATTGTCAATGCCTCT
CACCTCCTCACGTGCGGCACCTTCGCTTTTGATCCGAAGTGCGGGGTTATTGATGTGTCCAGTTTCCAGC
AGGTTGAAAGACTTGAGAGCGGCCGGGGGAAATGTCCTTTTGAGCCAGCTCAACGGTCAGCAGCTGTAAT
GGCTGGGGGCGTCCTCTACACCGCCACTGTGAAGAACTTCCTGGGGACTGAGCCCATCATCTCCCGAGCT
GTGGGTCGAGCTGAGGACTGGATTCGAACAGAGACCTTGTCATCCTGGCTTAATGCTCCAGCCTTTGTCG
CAGCTATGGTCCTGAGCCCAGCTGAGTGGGGGGATGAAGATGGAGACGATGAAATCTTTTTTTTCTTCAC
GGAGACCTCCCGAGTGTTGGACTCCTATGAGCGCATCAAGGTCCCAAGAGTGGCCCGAGTGTGTGCGGGG
GACCTTGGGGGCAGGAAGACCCTTCAGCAGAGATGGACGACGTTTCTGAAGGCTGACCTGCTGTGCCCAG
GGCCCGAGCATGGCCGGGCCTCCGGGGTTCTGCAGGCTATGGCAGAGCTTCGGCCTCAGCCTGGAGCGGG
AACCCCCATCTTTTATGGGATCTTTTCCTCCCAGTGGGAAGGAGCTGCCATCTCTGCTGTGTGTGCCTTC
CGACCCCAAGACATCCGGGCAGTGCTGAATGGTCCCTTTAGAGAGCTAAAACATGACTGCAACAGGGGAC
TGCCTGTCATGGACAACGAGGTGCCCCAGCCCAGACCTGGAGAGTGCATCGCCAACAACATGAAGCTCCA
GCAGTTTGGATCCTCACTCTCCCTGCCAGACCGCGTGCTCACCTTTATCAGAGACCACCCTCTCATGGAC
AGGCCCGTGTTCCCGGCTGACGGCCGCCCCCTGCTGGTCACTACAGATACAGCCTATCTCAGAGTCGTGG
CCCACAGGGTGACCAGCCTCTCAGGGAAAGAATATGACGTGCTCTACCTGGGGACAGAGGATGGACACCT
CCACCGGGCTGTGCGCATTGGAGCTCAGCTCAGTGTCTTGGAGGATCTGGCCTTGTTCCCAGAACCACAG
CCGGTTGAGAGCATGAAATTGTACCACGATTGGCTCCTGGTGGGCTCCCATACTGAGGTGACACAAGTGA
ACACCAGCAACTGTGGCCGTCTCCAGAGCTGCTCGGAGTGTATCCTGGCCCAGGACCCCGTGTGCGCCTG
GAGCTTCCGGCTTGATGCTTGTGTGGCCCACGCCGGCGAGCACCGCGGGATGGTTCAAGATATAGAGTCA
GCGGATGTCTCTTCTTTGTGTCCAAAAGAACCTGGAGAACATCCCGTAGTGTTTGAAGTTCCGGTGGCTA
CTGTGGGCCACGTGGTCCTGCCATGTTCCCCCAGTTCTGCCTGGGCATCCTGTGTGTGGCACCAGCCCAG
TGGAGTGACTGCGCTCACTCCCCGGAGGGATGGACTAGAGGTGGTGGTGACCCCAGGGGCCATGGGGGCT
TATGCTTGCGAGTGTCAGGAGGGTGGAGCCGCCCGCGTGGTGGCTGCTTATAGCTTGGTGTGGGGCAGCC
AGCGGGGACCCTCAAACCGGGCCCACACCGTTGTGGGGGCTGGATTGGTTGGCTTTCTCCTGGGTGTTCT
TGCAGCATCCCTCACTCTCCTCCTGATTGGTCGCCGTCAGCAGCGTCGGCGACAGAGGGAGCTTCTAGCT
AGAGACAAGGTGGGCTTAGATCTGGGGGCTCCACCTTCTGGGACCACAAGCTATAGTCAGGACCCTCCCT
CTCCTTCGCCTGAAGATGAACGGCTGCCCCTGGCCCTGGGTAAGCGGGGCAGTGGTTTTGGTGGCTTCCC
TCCACCCTTCCTGCTGGATTCTTGCCCAAGCCCAGCCCACATCCGGCTCACTGGGGCGCCTCTAGCCACG
TGTGATGAGACCTCCATC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
>RR205977 representing NM_019272
Red=Cloning site Green=Tags(s)

MLARAERPRPGPRPPPVFPFPPPLSLLLLLAILSAPVCGRVPRSVPRTSLPISEADSYLTRFAASHTYNY
SALLVDPASHTLYVGARDSIFALTLPFSGERPRRIDWMVPETHRQNCRKKGKKEDECHNFIQILAIVNAS
HLLTCGTFAFDPKCGVIDVSSFQQVERLESGRGKCPFEPAQRSAAVMAGGVLYTATVKNFLGTEPIISRA
VGRAEDWIRTETLSSWLNAPAFVAAMVLSPAEWGDEDGDDEIFFFFTETSRVLDSYERIKVPRVARVCAG
DLGGRKTLQQRWTTFLKADLLCPGPEHGRASGVLQAMAELRPQPGAGTPIFYGIFSSQWEGAAISAVCAF
RPQDIRAVLNGPFRELKHDCNRGLPVMDNEVPQPRPGECIANNMKLQQFGSSLSLPDRVLTFIRDHPLMD
RPVFPADGRPLLVTTDTAYLRVVAHRVTSLSGKEYDVLYLGTEDGHLHRAVRIGAQLSVLEDLALFPEPQ
PVESMKLYHDWLLVGSHTEVTQVNTSNCGRLQSCSECILAQDPVCAWSFRLDACVAHAGEHRGMVQDIES
ADVSSLCPKEPGEHPVVFEVPVATVGHVVLPCSPSSAWASCVWHQPSGVTALTPRRDGLEVVVTPGAMGA
YACECQEGGAARVVAAYSLVWGSQRGPSNRAHTVVGAGLVGFLLGVLAASLTLLLIGRRQQRRRQRELLA
RDKVGLDLGAPPSGTTSYSQDPPSPSPEDERLPLALGKRGSGFGGFPPPFLLDSCPSPAHIRLTGAPLAT
CDETSI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_019272
ORF Size 2328 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_019272.1, NP_062145.1
RefSeq Size 4008 bp
RefSeq ORF 2331 bp
Locus ID 29745
UniProt ID Q9Z143
MW 84.3 kDa
Gene Summary has axon growth-cone collapse activity [RGD, Feb 2006]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

SKU Description Size Price
RN205977 Sema4f (untagged ORF) - Rat sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4F (Sema4f), (10 ug)
    • 10 ug

CNY 9,980.00

RR205977L3 Lenti ORF clone of Sema4f (Myc-DDK-tagged ORF) - Rat sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4F (Sema4f), (10 ug)
    • 10 ug

CNY 11,780.00

RR205977L4 Lenti ORF clone of Sema4f (mGFP-tagged ORF) - Rat sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4F (Sema4f), (10 ug)
    • 10 ug

CNY 12,920.00

You may also need
plasmid icon Empty Vector
protein iconDDK/Flag Antibody

其它Sema4f产品

Expression Plasmids
Lentiviral Particles
CRISPR
RNAi
Proteins
Antibodies
相关产品 (8)

PS100001

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RR205977L4

Lenti ORF clone of Sema4f (mGFP-tagged ORF) - Rat sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4F (Sema4f), (10 ug)

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CNY 12,920.00

RR205977L4V

Lenti ORF particles, Sema4f (GFP-tagged ORF) - Rat sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4F (Sema4f), 200ul, >10^7 TU/mL

    • 200 ul

CNY 14,060.00 暂停销售

RR205977L3

Lenti ORF clone of Sema4f (Myc-DDK-tagged ORF) - Rat sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4F (Sema4f), (10 ug)

    • 10 ug

CNY 11,780.00

RR205977L3V

Lenti ORF particles, Sema4f (Myc-DDK-tagged ORF) - Rat sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4F (Sema4f), 200ul, >10^7 TU/mL

    • 200 ul

CNY 12,654.00 暂停销售

RN205977

Sema4f (untagged ORF) - Rat sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4F (Sema4f), (10 ug)

    • 10 ug

CNY 9,980.00

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