Mtnr1b (NM_001100641) Rat Tagged ORF Clone

CAT#: RR205881

  • TrueORF®

Mtnr1b (Myc-DDK-tagged ORF) - Rat melatonin receptor 1B (Mtnr1b), (10 ug)



  "NM_001100641" in other vectors (3)

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CNY 3,990.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Synonyms Mel1b-r
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR205881 representing NM_001100641
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTGACAACAGCTCCATCGCCAACTGCTGTGCGGCCAGCGGGCTGGCAGCGCGCCCTAGTTGGCCCG
GGTCAGCGGAGGCGGAGCCCCCTGAGACTCCCCGGGCACCCTGGGTGGCTCCCATGCTATCTACAGTAGT
CATTGTCACCACAGCTGTGGACTTCGTGGGGAACCTGCTTGTGATCCTCTCGGTGCTCAGGAACCGCAAG
CTGCGGAACGCAGGTAATTTATTTGTGGTGAATCTGGCCTTGGCTGACCTGGTGGTAGCCCTGTACCCTT
ACCCACTCATCCTTGTGGCCATTCTCCATGACGGTTGGGTACTTGGGGAGATCCACTGTAAGGCCAGTGC
CTTTGTGATGGGCCTGAGTGTCATTGGCTCTGTCTTCAACATCACAGCCATTGCCATCAACCGCTACTGG
TGCATCTGTCACAGTGCGACCTACCACCGAGCCTGCAGTCAGTGGCATGCTCCCCTCTACATCAGCCTCA
TCTGGCTTCTCACTCTGGTGGCCTTGGTGCCCAATTTCTTTGTGGGGTCTCTAGAATATGACCCGCGAAT
CTATTCCTGCACCTTCATCCAGACAGCCAGCACCCAATACACTATGGCTGTGGTGGCCATCCACTTCCTC
CTTCCAATTGCTGTGGTGTCCTTTTGCTACCTGCGAATATGGATACTGGTGCTCCAGGCCCGAAGGAAGG
CAAAGGCTGAGAGAAAGCTACGCCTGAGACCCAGTGACCTGCGCAGTTTCCTAACCATGTTCGCAGTGTT
TGTGGTTTTCGCCATATGCTGGGCCCCCCTCAACTGTATTGGCCTTGCAGTGGCCATCAATCCAGAGGCA
ATGGCTCTTCAGATCCCAGAAGGGCTTTTTGTCACCAGTTACTTCCTAGCTTACTTCAACAGCTGCCTTA
ATGCTATTGTTTATGGGCTTCTGAACCAGAACTTCCGCAGGGAGTACAAGAGGATCCTCTCGGCCCTCTG
GAGCACTGGGCGCTGCTTCCATGATGCTTCCAAATGCCACCTGACTGAGGATTTGCAGGGCCCAGTGCCA
CCTGCTGCCATGGCCACCATACCTGTCCAGGAAGGTGCTCTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR205881 representing NM_001100641
Red=Cloning site Green=Tags(s)

MPDNSSIANCCAASGLAARPSWPGSAEAEPPETPRAPWVAPMLSTVVIVTTAVDFVGNLLVILSVLRNRK
LRNAGNLFVVNLALADLVVALYPYPLILVAILHDGWVLGEIHCKASAFVMGLSVIGSVFNITAIAINRYW
CICHSATYHRACSQWHAPLYISLIWLLTLVALVPNFFVGSLEYDPRIYSCTFIQTASTQYTMAVVAIHFL
LPIAVVSFCYLRIWILVLQARRKAKAERKLRLRPSDLRSFLTMFAVFVVFAICWAPLNCIGLAVAINPEA
MALQIPEGLFVTSYFLAYFNSCLNAIVYGLLNQNFRREYKRILSALWSTGRCFHDASKCHLTEDLQGPVP
PAAMATIPVQEGAL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001100641
ORF Size 1092 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001100641.1, NP_001094111.1
RefSeq Size 1858 bp
RefSeq ORF 1095 bp
Locus ID 192646
UniProt ID P49287
MW 40.2 kDa
Gene Summary G protein-coupled receptor for the hormone melatonin [RGD, Feb 2006]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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