Mast3 (NM_001134796) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR205714 representing NM_001134796 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGACCCCAGCTACTGGACCGCGGTGGCGGCCCCCGGACACCGCAGCCGCCTGGCCAAGGGTGCGC TGCTACAACGTTCCAAAAGCTGCCGCAGTGGCAACCGTAAGAGCCTAGTCGTGGGGACGCCTTCACCCAC TCTCTCCAGACCTTTGTCACCACTCTCTGTCCCAACTGCAGGTAACAGCCCCTTGGACAGTCCTCGGAAC TTCTCTGCCGCAGCGGCCATCAATTTCCCCTTTGCCCGGAGGGCTGACGGCAGAAGATGGTCACTTGCTT CCCTCCCGTCATCAGGCTATGGGACCAACACTCCCAGCTCCACTGTGTCGTCAAGCTCATCCTCCCGGGA GCGTCTACACCAGCTCCCTTTCCAACCCACTGCGGACGAACTGCGCTTCCTGTCCAAGCACTTCCGCAGC TCAGAGAGTGTGGTGGATGAGGATGGAGGCCGTTCTCCGCGCCTGCGCCCACGCTCACGAAGCCTCAGCC CTGGACGAACATCAGGAACCTTCGACAACGAGATAGTGATGATGAATCATGTATACCGGGAGCGCTTCCC CAAGGCCACAGCGCAGATGGAGGGCCGGCTGCAGGACTTCCTGGCAGCATTTGCTCCTGGAGACCGCCTG GCACTGGCAGACGGTGTCCTGGGCTTCATCCACCACCAGATCGTGGAGTTGGCGAGGGACTGCCTGGCCA AGTCGGGCGAGGCTCTAGTCACCTCCCGCTATTTCCTGGAGATGCAAGACAAGCTGGAGCGACTACTGCA GGATGCTCATGAGAGGTCAGATAGCGCCGAAGTGGGCTTCATCGTGCAGTTGGTCCGCAAGCTGCTCATC ATCATCTCCAGGCCAGCGCGGCTGCTCGAGTGCTTGGAGTTCGACCCCGAAGAATTCTACCATCTGCTGG AGGCTGCAGAGGGCCAGGCCCGCGAGGACCAGGGCGTGAAGACTGATCTGCCCCGCTACATCATCCGCCA GCTGGGCCTTGCCAAGGACCCTCTAGAAGAGATTCAGCCCCTGAATGACCTTGATGAAGGCCAGCCACCA GCTCCTGGATCCCCTGAGAGCCGAGGCCTGGGCGGTCCGTCTCGGAGGCAGCCATGTGAGAGCGACTTTG AGACCATCAAGCTTATTAGCAATGGAGCCTATGGGGCTGTCTACCTGGTGCGCCACAGGGACACACGACA GCGCTTCGCCATCAAGAAGATCAACAAGCAGAATTTGATTCTGCGCAACCAGATCCAACAGGTGTTTGTG GAGCGAGACATCCTCACCTTCGCCGAGAACCCCTTTGTGGTCGGCATGTTCTGCTCTTTTGAAACCCGTC GCCACCTCTGCATGGTGATGGAGTATGTGGAAGGGGGTGACTGCGCCACGCTGCTGAAAAACATGGGCCC ACTGCCTGTGGACATGGCCCGCATGTACTTCGCGGAGACGGTACTGGCTCTGGAGTATCTGCACAACTAT GGCATCGTGCACCGAGACCTCAAACCTGACAACCTGCTCATCACCTCCCTCGGCCACATCAAGCTGACGG ACTTCGGCCTGTCCAAGATCGGCCTCATGAGCATGGCCACCAACCTGTATGAAGGTCACATCGAGAAGGA TGCTCGAGAGTTTGTGGACAAGCAGGTGTGCGGGACGCCTGAATACATCGCGCCAGAGGTGATCTTCCGC CAGGGCTATGGGAAGCCAGTAGACTGGTGGGCCATGGGCGTCATCCTGTATGAGTTCCTGGTGGGCTGCG TGCCTTTCTTTGGGGACACACCTGAGGAGCTGTTCGGACAGGTTGTCAGTGATGAGATCATGTGGCCAGA GGGAGATGAGGCCCTTCCCCCCGACGCCCAGGATCTCATTACTAGGCTTCTGCGTCAGAGCCCAATGGAC AGGCTGGGGACTGGAGGCACCCACGAGGTGAAACAGCACCCCTTCTTCCTGGCCCTGGACTGGGCAGGGC TCCTGAGACACAAGGCAGAGTTTGTCCCACAGCTTGAAGCTGAGGACGACACCAGCTACTTTGACACTCG CTCTGAGCGGTACCGCCACTTGGGCTCCGAGGGTGACGAGACGAATGACGAGGAATCCTCCACAGAGATC CCCCAGTTCTCTTCCTGTTCCCACCGCTTCAGCAAGGTCTACAGCAGTTCCGAGTTCCTGGCTGTCCAGC CCAACCCCACCTTTGCTGAAAGAAGCTTCAGTGAGGACCGAGAGGAAGGTTGGGAGCAAAGCGGTGAAGG GGACGGTGGCCGCCGGCTGAGCACTGACCTCCGGCTGAGGTCCTGGACACCTGCTTCCTCCTGTCAGCCG TCTTCCTGTCAGAGCGACCGGGGTCCCAGCCCGTCTCTCCTAAGCACCATCAGCTTGGACATGCCTAAAT TTGCCTTCTCCTCTGAAGATGAGGGAGCAGGCTCAGGGCCTGCTGACACCAGAAAGCCCGTCTTCATCTT GGGGGAGCCTGACCCCCCACCCCCGACCACCCCAGTGACACCTAAGCCCTGCAACCTTTCTGCCGACACA GCTGTTCTCAGCCATGCCCGCCTTCGAAGTAACAGTACAGGCGCCCGGCACTCAACTCCTCGGCCCCTGG ATGCCGGCAGAGGCCGCCGCCTAGGAAGCTCAAGAGACCCCGGCCCAGAGAAGCCCAGGGCCTCCCCTGG TGGCAGCGGGGGTCGAGTGCCCAAGTCAGCCTCTGTGTCTGCCCTGTCCCTCATCATCACGGCAGACGAC GGCAGTGGGGGCCCCCTCATGAGTCCCTTGTCGCCCCGCTCGCTGTCCTCCAACCCCTCCTCCCGAGACT CCTCACCTAGCCGGGACCCATCACCAGTGTGTGGTAGCCTGCGGCCCCCCATCGTCATCCACAGCTCGGG CAAGAAGTACGGCTTCAGCCTTCGCGCCATTCGTGTCTACATGGGAGACAGCGACGTGTACACGGTGCAC CATGTGGTCTGGAGCGTGGAGGAGGGGAGCCCTGCGCAGGAGGCGGGACTGCGTGCTGGGGACCTCATCA CACACATCAACGGGGAATCTGTCCTGGGCCTGGTGCACATGGATGTGGTGGAGCTGCTGCTGAAGAGTGG CAACAAGATCTCCCTGCGCACCACGGCCCTGGAGAACACCTCCATCAAGGTGGGGCCTGCCCGGAAGAAC GTGGCCAAGGGCCGCATGGCCCGAAGAAGCAAGCGCAGCCGCCGGCGCGAGACCCAGGACCGGCGGAAGT CCTTGTTTAAGAGGATCTCGAAGCCATCTTCTGTGCTCCACACCAGCCGTAGTTTCTCCTCGGGCCTCCA GCACTCTCTGTCCTCCAGCGAGAGCCTCCCTGGATCACCCACTCACAGCCTGTCCCCTAGCCCCACCACG CCCTGCCGCAGCCCTGCCCCCGACGTCCCCACAGACACAGCATCCCCACCCAGTGTATCCCCAAGTTCCA GCAGTCCCGCTTCTCCAGCAACTGGCCACACCCGTCCCAGCTCACTTCATGGCCTGGCAGCCAAGCTCGG GCCACCTCGACACAAGAGCGGCCGCCGCAAGTCCACAAGCAGTATCCCTCCGTCACCACTGGCTTGCCCG CCAGTGCCCACACCCCCACCACGCTCGCCGTCTCCCCTTCCTGGGCACATTCCCATACCAGCACGGTCCC CTAGGCTGCGTAGGGGCCAGTCTGCCGATAAGCTGGGCCTAGGCACCAGTGAGAGGCTGGATGGGGACGG AGGCCGGCGTGGCAGAGGGGCCGAGACTGAGCTGGTCGTCATGAGGAGGCTGCATCTGTCAGAGCGCCGG GACTCCTTCAAGAAGCAGGAGGCCGTGCAGGAAGTGAGCTTCGATGAGGAGCCTGGGCCGACTGGTGCGG TGCCGAAGATCGCTGTGCAGGGTGCAGAGGCCACGCCTGGAAGCCCAGGACCAGCCAGGGAAGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR205714 representing NM_001134796 Red=Cloning site Green=Tags(s) MSDPSYWTAVAAPGHRSRLAKGALLQRSKSCRSGNRKSLVVGTPSPTLSRPLSPLSVPTAGNSPLDSPRN FSAAAAINFPFARRADGRRWSLASLPSSGYGTNTPSSTVSSSSSSRERLHQLPFQPTADELRFLSKHFRS SESVVDEDGGRSPRLRPRSRSLSPGRTSGTFDNEIVMMNHVYRERFPKATAQMEGRLQDFLAAFAPGDRL ALADGVLGFIHHQIVELARDCLAKSGEALVTSRYFLEMQDKLERLLQDAHERSDSAEVGFIVQLVRKLLI IISRPARLLECLEFDPEEFYHLLEAAEGQAREDQGVKTDLPRYIIRQLGLAKDPLEEIQPLNDLDEGQPP APGSPESRGLGGPSRRQPCESDFETIKLISNGAYGAVYLVRHRDTRQRFAIKKINKQNLILRNQIQQVFV ERDILTFAENPFVVGMFCSFETRRHLCMVMEYVEGGDCATLLKNMGPLPVDMARMYFAETVLALEYLHNY GIVHRDLKPDNLLITSLGHIKLTDFGLSKIGLMSMATNLYEGHIEKDAREFVDKQVCGTPEYIAPEVIFR QGYGKPVDWWAMGVILYEFLVGCVPFFGDTPEELFGQVVSDEIMWPEGDEALPPDAQDLITRLLRQSPMD RLGTGGTHEVKQHPFFLALDWAGLLRHKAEFVPQLEAEDDTSYFDTRSERYRHLGSEGDETNDEESSTEI PQFSSCSHRFSKVYSSSEFLAVQPNPTFAERSFSEDREEGWEQSGEGDGGRRLSTDLRLRSWTPASSCQP SSCQSDRGPSPSLLSTISLDMPKFAFSSEDEGAGSGPADTRKPVFILGEPDPPPPTTPVTPKPCNLSADT AVLSHARLRSNSTGARHSTPRPLDAGRGRRLGSSRDPGPEKPRASPGGSGGRVPKSASVSALSLIITADD GSGGPLMSPLSPRSLSSNPSSRDSSPSRDPSPVCGSLRPPIVIHSSGKKYGFSLRAIRVYMGDSDVYTVH HVVWSVEEGSPAQEAGLRAGDLITHINGESVLGLVHMDVVELLLKSGNKISLRTTALENTSIKVGPARKN VAKGRMARRSKRSRRRETQDRRKSLFKRISKPSSVLHTSRSFSSGLQHSLSSSESLPGSPTHSLSPSPTT PCRSPAPDVPTDTASPPSVSPSSSSPASPATGHTRPSSLHGLAAKLGPPRHKSGRRKSTSSIPPSPLACP PVPTPPPRSPSPLPGHIPIPARSPRLRRGQSADKLGLGTSERLDGDGGRRGRGAETELVVMRRLHLSERR DSFKKQEAVQEVSFDEEPGPTGAVPKIAVQGAEATPGSPGPARED TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001134796
ORF Size	3915 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001134796.1, NP_001128268.1
RefSeq Size	5371 bp
RefSeq ORF	3918 bp
Locus ID	688540
MW	142.4 kDa

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