Rims1 (NM_052829) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Rim1; RIM1a
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR205684 representing NM_052829 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCTCGGCCGTGGGGCCCCGAGGTCCTCGCCCACCCACGGTGCCTCCCCCTATGCAAGAACTGCCCG ACCTGAGCCACCTGACCGAGGAGGAGAGGAACATTATCATGGCAGTGATGGACCGGCAGAAGGAAGAGGA GGAAAAAGAAGAGGCCATGCTCAAGTGTGTTGTCAGGGACATGGCGAAGCCTGCTGCCTGCAAAACACCA AGAAATGCTGAAAGCCAGCCCCATCAACCACCACTGAACATTTTCAGATGTGTCTGTGTTCCCAGAAAGC CAAGCAGCGAAGAGGGAGGCCCAGAAAGAGACTGGAGATTGCATCAACAGTTTGAAAGCTACAAGGAGCA AGTGAGAAAAATCGGAGAGGAAGCGAGGCGTTACCAGGGCGAGCACAAGGATGATGCCCCGACGTGTGGA ATCTGTCATAAGACAAAGTTTGCTGATGGATGTGGCCATCTCTGCTCCTATTGTCGCACCAAGTTCTGTG CACGCTGCGGAGGCCGTGTGTCTCTGCGATCGAACAATGAGGACAAAGTGGTTATGTGGGTATGCAATTT ATGTCGAAAGCAACAAGAAATCTTAACGAAATCTGGAGCGTGGTTCTTTGGAAGTGGCCCTCAGCAGCCT AGTCAAGATGGGACTCTGAGTGACACGGCCACAGGTGCTGGATCTGAGGTGCCAAGAGAAAAGAAAGCAA GGCTCCAAGAGCGATCAAGGTCTCAGACGCCCTTGAGTACAGCAGCTGTCTCTTCCCAAGACACTGCTAC CCCCGGTGCACCGTTGCACAGGAACAAAGGGGCTGAGCCCTCACAGCAAGCCTTGGGTCCTGAACAGAAG CAGGCATCAAGATCAAGAAGCGAGCCACCGAGGGAAAGGAAGAAGGCTCCAGGGCTTTCAGAGCAGAATG GCAAGGGAGGCCAGAAGAGCGAGCGCAAACGTGTCCCCAAGTCTGTGGTGCAACCCGGGGAAGGGATCGC GGATGAGAGGGAGAGGAAAGAGAGGCGGGAAACCCGCAGGTTGGAGAAAGGGCGCTCCCAGGACTACTCA GACCGGCCTGAGAAACGCGACAATGGCAGGGTGGCGGAAGACCAGAAGCAGAGGAAGGAGGAGGAGTACC AGACTAGGTACCGCAGCGACCCTAACCTGGCTCGCTACCCGGTGAAGGCGCCGCCAGAGGAGCAGCAGAT GCGCATGCACGCCCGGGTGTCCCGAGCGAGGCACGAGCGGCGCCACAGCGACGTGGCGCTCCCGCACACC GAGGCAGCTGCCGCCGCGCCGGCTGAGGCCACGGCGGGCAAGCGCGCGCCGGCCACCGCCAGGGTCTCTC CCCCGGAGTCCCCGCGCGCACGCGCGGCGGCCGCCCAGCCTCCCACCGAGCACGGGCCACCGCCGCCGCG GCCAGCCCCGGGTCCCGCAGAGCCACCCGAGCCGCGCGTCCCCGAGCCGCTCCGTAAGCAGGGCCGCCTG GACCCGGGCTCGGCCGTGCTTCTGCGCAAGGCCAAGCGCGAGAAGGCGGAGAGCATGCTGCGGAACGACT CGCTGAGCTCCGATCAGTCCGAGTCCGTGCGGCCATCCCCGCCCAAGCCTCACCGGCCCAAGCGGGGAGG CAAGAGACGTCAGATGTCGGTGAGCAGCTCGGAGGAGGAGGGCGTGTCCACACCGGAGTACACGAGCTGC GAGGACGTGGAGCTGGAGAGCGAGAGCGTGAGCGAGAAAGGTGACTTGGATTACTACTGGTTGGATCCCG CCACGTGGCACAGCAGGGAAACGTCGCCTATCAGTTCGCATCCTGTAACGTGGCAGCCGTCTAAAGAGGG AGATCGACTAATCGGCCGTGTTATTCTTAACAAAAGAACAACCATGCCCAAAGAATCAGGTGCATTATTG GGTCTGAAGGTGGTTGGAGGAAAAATGACGGACTTAGGGCGCCTTGGTGCTTTCATCACCAAAGTAAAGA AGGGCAGCCTGGCAGACGTCGTCGGACACCTAAGAGCAGGGGACGAAGTCCTAGAGTGGAATGGTAAACC CCTGCCGGGAGCAACAAACGAAGAAGTTTACAACATTATCTTAGAATCAAAATCAGAACCTCAAGTTGAG ATTATTGTTTCAAGGCCTATTGGTGACATCCCCAGGATCCCTGAGAGTTCCCATCCTCCCCTGGAGTCCA GTTCAAGTTCCTTTGAATCTCAGAAAATGGAAAGGCCTTCTATTTCTGTTATTTCTCCAACCAGCCCTGG AGCTCTGAAAGATGCCCCACAAGTCTTACCAGGGCAACTCTCAGTGAAGCTATGGTATGATAAAGTGGGG CACCAGCTGATTGTAAATGTTCTACAAGCAACAGATCTACCCCCTAGAGTAGATGGCCGTCCCAGGAATC CCTATGTAAAAATGTATTTTCTTCCAGATAGAAGCGACAAAAGTAAAAGGAGAACCAAAACAGTAAAGAA ACTTCTAGAGCCAAAATGGAACCAGACATTTGTCTACTCACACGTACATCGTAGAGATTTTCGAGAGCGA ATGTTAGAGATTACCGTGTGGGACCAGCCGAGAGTACAGGACGAAGAGAGTGAATTTCTTGGAGAGATCC TCATAGAGTTGGAAACAGCGCTTTTAGATGATGAGCCCCATTGGTATAAACTCCAGACACATGACGAATC TTCACTACCTCTGCCTCAGCCATCACCGTTCATGCCCAGGCGGCATATTCATGGAGAGAGCTCCAGCAAA AAGCTACAAAGATCTCAGCGAATCAGTGATAGTGACATCTCAGATTATGAGGTTGATGATGGTATTGGAG TAGTGCCTCCAGTGGGTTATAGAGCTAGTGCTAGAGAGAGTAAAGCAACCACGTTAACAGTGCCAGAGCA ACAAAGAACTACACATCACCGCTCACGTTCCGTGTCTCCTCATCGCGGCGATGATCAGGGAAGGCCTCGT TCACGTTTACCAAATGTGCCATTACAGAGGAGCTTAGATGAAATTCATCCAACACGAAGGTCACGTTCTC CAACCCGACACCATGATGCCTCCCGAAGCCCGGCCGATCACAGATCCAGACATGTGGAAAGTCAATATTC GTCAGAGCCAGACAGTGAGCTTCTCATGCTGCCCAGAGCAAAACGAGGACGAAGTGCAGAAAGCCTACAC ATGACCAGTGAACTGCAGCCCTCTCTTGACAGGGCTAGGAGTGCTAGTACCAACTGCTTGAGACCAGATA CTAGTTTGCATTCACCAGAACGAGAAAGGCACTCCAGAAAGTCTGAAAGATGTAGCATCCAAAAACAGTC TAGGAAAGGCACAGCCTCTGATGCAGACAGAACGCACCGACAAGGAAGCCCAACCCAGTCTCCTCCAGCA GACACATCCTTCGGCAGTCGCCGTGGAAGACAGCTCCCACAGGTGCCAGTTCGAAGCGGCAGTATAGAAC AAGCAAGCTTAGTAGTGGAGGAGCGAACGAGACAGATGAAAGTGAAAGTTCACCGATTTAAGCAGACAAC AGGGTCTGGGTCTAGTCAAGAACTTGACCACGAGCAATACTCCAAGTACAACATACATAAAGATCAGTAC AGAAGCTGTGATAACGCGTCTGCCAAGTCTTCAGATAGTGATGTCAGTGATGTGTCCGCCATTTCCAGAG CCAGCAGTACCTCACGCCTCAGCAGCACAAGCTTTATGTCAGAGCAGTCTGAGCGCCCCAGGGGTAGGAT CAGTTCATTTACCCCCAAAATGCAAGGCAGACGGATGGGGACTTCAGGAAGAGCCATCATCAAGAGCACC AGTGTAAGTGGAGAGATATATACACTGGAACGTAATGACGGTAGCCAGTCGGACACGGCCGTAGGTACCG TCGGAGCCGGTGGAAAGAAACGAAGATCCAGCCTGAGCGCCAAAGTGGTAGCCATTGTGTCTCGAAGAAG CAGGAGCACGTCACAGCTCAGCCAGACAGAGTCGGGCCACAAGAAGTTGAAAAGCACCATCCAGAGGAGT ACGGAAACAGGAATGGCAGCTGAAATGCGGAAGATGGTGAGACAGCCGAGCCGGGAGTCCACGGATGGCA GCATCAACAGTTATAGCTCGGAAGGAAACTTGATATTTCCTGGAGTTCGAGTAGGACCCGACAGTCAGTT CAGTGATTTCCTTGATGGGTTGGGACCAGCGCAGCTCGTTGGCCGTCAGACGCTCGCCACCCCGGCCATG GGCGATATCCAAATCGGGATGGAGGATAAGAAGGGTCAGTTGGAGGTTGAGGTTATCAGAGCCCGGAGCC TTACACAAAAACCTGGTTCCAAATCTACACCCGCTCCCTATGTGAAAGTATATCTTTTGGAAAATGGAGC CTGTATTGCCAAAAAGAAGACAAGAATTGCACGGAAAACTCTCGATCCTTTGTATCAGCAGTCCCTGGTT TTTGATGAAAGTCCACAGGGTAAAGTTCTTCAGGTGATTGTCTGGGGTGACTATGGAAGAATGGACCACA AATGCTTTATGGGTGTGGCTCAAATCTTGTTGGAAGAACTTGATCTATCCAGCATGGTGATTGGATGGTA TAAATTGTTCCCTCCGTCCTCACTGGTGGATCCCACTCTCGCTCCCCTGACCCGCCGGGCTTCCCAATCA TCTCTGGAAAGTTCGTCCGGGCCTCCCTGCATCCGGTCA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR205684 representing NM_052829 Red=Cloning site Green=Tags(s) MSSAVGPRGPRPPTVPPPMQELPDLSHLTEEERNIIMAVMDRQKEEEEKEEAMLKCVVRDMAKPAACKTP RNAESQPHQPPLNIFRCVCVPRKPSSEEGGPERDWRLHQQFESYKEQVRKIGEEARRYQGEHKDDAPTCG ICHKTKFADGCGHLCSYCRTKFCARCGGRVSLRSNNEDKVVMWVCNLCRKQQEILTKSGAWFFGSGPQQP SQDGTLSDTATGAGSEVPREKKARLQERSRSQTPLSTAAVSSQDTATPGAPLHRNKGAEPSQQALGPEQK QASRSRSEPPRERKKAPGLSEQNGKGGQKSERKRVPKSVVQPGEGIADERERKERRETRRLEKGRSQDYS DRPEKRDNGRVAEDQKQRKEEEYQTRYRSDPNLARYPVKAPPEEQQMRMHARVSRARHERRHSDVALPHT EAAAAAPAEATAGKRAPATARVSPPESPRARAAAAQPPTEHGPPPPRPAPGPAEPPEPRVPEPLRKQGRL DPGSAVLLRKAKREKAESMLRNDSLSSDQSESVRPSPPKPHRPKRGGKRRQMSVSSSEEEGVSTPEYTSC EDVELESESVSEKGDLDYYWLDPATWHSRETSPISSHPVTWQPSKEGDRLIGRVILNKRTTMPKESGALL GLKVVGGKMTDLGRLGAFITKVKKGSLADVVGHLRAGDEVLEWNGKPLPGATNEEVYNIILESKSEPQVE IIVSRPIGDIPRIPESSHPPLESSSSSFESQKMERPSISVISPTSPGALKDAPQVLPGQLSVKLWYDKVG HQLIVNVLQATDLPPRVDGRPRNPYVKMYFLPDRSDKSKRRTKTVKKLLEPKWNQTFVYSHVHRRDFRER MLEITVWDQPRVQDEESEFLGEILIELETALLDDEPHWYKLQTHDESSLPLPQPSPFMPRRHIHGESSSK KLQRSQRISDSDISDYEVDDGIGVVPPVGYRASARESKATTLTVPEQQRTTHHRSRSVSPHRGDDQGRPR SRLPNVPLQRSLDEIHPTRRSRSPTRHHDASRSPADHRSRHVESQYSSEPDSELLMLPRAKRGRSAESLH MTSELQPSLDRARSASTNCLRPDTSLHSPERERHSRKSERCSIQKQSRKGTASDADRTHRQGSPTQSPPA DTSFGSRRGRQLPQVPVRSGSIEQASLVVEERTRQMKVKVHRFKQTTGSGSSQELDHEQYSKYNIHKDQY RSCDNASAKSSDSDVSDVSAISRASSTSRLSSTSFMSEQSERPRGRISSFTPKMQGRRMGTSGRAIIKST SVSGEIYTLERNDGSQSDTAVGTVGAGGKKRRSSLSAKVVAIVSRRSRSTSQLSQTESGHKKLKSTIQRS TETGMAAEMRKMVRQPSRESTDGSINSYSSEGNLIFPGVRVGPDSQFSDFLDGLGPAQLVGRQTLATPAM GDIQIGMEDKKGQLEVEVIRARSLTQKPGSKSTPAPYVKVYLLENGACIAKKKTRIARKTLDPLYQQSLV FDESPQGKVLQVIVWGDYGRMDHKCFMGVAQILLEELDLSSMVIGWYKLFPPSSLVDPTLAPLTRRASQS SLESSSGPPCIRS SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_052829
ORF Size	4659 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_052829.1, NP_439894.1
RefSeq Size	5655 bp
RefSeq ORF	4662 bp
Locus ID	84556
UniProt ID	Q9JIR4
MW	173 kDa
Gene Summary	interacts with synaptic GTP-binding protein Rab3s; may regulate exocytosis at the synapse [RGD, Feb 2006]

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