United States
Germany
Japan
United Kingdom
China
Bbs2 (NM_053618) Rat Tagged ORF Clone
CAT＃: RR205555
- TrueORF®
Bbs2 (Myc-DDK-tagged ORF) - Rat Bardet-Biedl syndrome 2 homolog (human) (Bbs2), (10 ug)
"NM_053618" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 9,220.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR205555 representing NM_053618
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCTGCCTGTGTTCACCCTGAAACTGCGCCACAAAATCAGCCCCCGCATGGTGGCCATAGGGCGCT ACGACGGGACTCACCCGTGCTTGGCGGCCGCCACCCAAGCGGGCAAGGTTTTTATTCATAATCCTCACAT GCGGAGCCAGCATTTTAGTACATCCAGAGTGTTCCAGAGCCCTCTTGAGTCTGATGTGTCCCTCCTCAAC ATTAACCAGACAGTCAGCTGCCTCGGCGCCGGAGTCTTGAACCCTGAGCTTGGCTATGATACTCTCTTAG TGGGGACACAGACCAGTCTTTTGGCTTACGACATCTACAATAATTCAGATTTGTTCTACAGAGAGGTCGC GGATGGGGCAAATGCCCTTGTGTTGGGGACTCTGGGGGACATTGCCCCGCCCCTTGCAATCATCGGTGGA AATTGTGCTCTTCAGGGTTTTGATCACGAAGGAAATGACCTCTTTTGGACGGTTACTGGAGACAATGTTC ATTCCTTGGCTCTGTGTGACTTTGACGGTGATGGGAAGTCAGAGCTTCTTGTTGGGTCTGAAGATTTTGA CATTCGAGTTTTTAAAGAAGATGAGATTGTGGCAGAAATGACAGAGACAGAGATAGTCACCTCTCTGTGT CCCATGTACGGCAGTCGGTTTGGTTATGCCCTTTCTAATGGCACAGTTGGAGTTTATGACAAAACAGCCC GGTACTGGAGAATTAAATCCAAAAACCATGCCATGAGCATCCATGCTTTTGACATCAATTCTGATGGAGT GTGTGAATTGATCACTGGGTGGTCTAATGGGAAGGTTGACGCTCGCAGTGACCGGACCGGAGAGGTCATA TTTAAAGACAACTTCTCTTCTGCCGTTGCTGGTGTGGTGGAGGGAGATTACCGGATGGATGGCCACGTAC AGTTAATCTGCTGCTCAGTGGATGGGGAAATCCGAGGCTACCTGCCAGGCACAGCTGAGATGAAGGGGAA CCTCCTGGACACAAGTGTGGAGCAGGGCCTGATCCGAGAGCTCAGTCAGAAAAAGCAGAACCTGTTGCTA GAACTGCGCAACTACGAGGAGAACACCAAGGCAGAACTGAGCAGTCCCCTGAATGAGGCCGATGGACAGA AAGGCATAATCCCAGCCAATACCAAGCTCCACACAGCCCTCTCAGTCAATCTGGGGAATGATGCACAAGA CGCACATGCGGAATTACGCATTTCCACTTCTAATGACACAATCATCCGGGCAGTACTCATTTTTGCAGAG GGAATTTTTGCGGGTGAAAGCCACGTGGTCCACCCCAGCACTCACAACCTTTCCAGCTCCATCCGGGTAC CAATTACGCCTCCCAAAGATGTCCCTGTGGATCTGCACTTGAAAACCTTCGTGGGTTACAGAAGCAGCAC CCAGTTCCATGTGTTTGAACTGATAAGGCAGCTGCCCAGATTTACCATGTATGCACTGACAAGCCCAGAC GCAGCGAGCGAGCCTGTCAGTTTCGTGAACTTCATCGTAGTAGAGCGGGCACAGAGGATGGTCACGTGGC TCAACCAGAACTTCTTGTTGCCAGAAGACAGTAACATTCAGAACGCCCCGTTTCACGTCTGTTTCACGTC CTTACGCAACGGTGGCCAGCTCTACATAAAAATGAAACCAAGTGGTGAGATCACGGTGAATACTGATGAT ATTGATCTTGCTGGTGATATCATCCAGTCGATGGCATCCTTTTTTGCTATTGAAGACCTTCAGGTAGAAG CAGATTTCCCTGTCTACTTCGAGGAGTTACGGAAAGTGCTGTTGAAGGTAGATGAGTACCATTCAGTGCA TCAAAAGCTCAGCGCCAACATGGCTGATAATTCTAACCTCATCCGAAGTTTGCTGGTCAGAGCGGAGGAT GCTCGTCTGATGAGAGACATGAAAACAATGAAGACTCGTTATATGGAGCTCTATGACCTTAATAAAGATT TGCTAAATGGATATAAGATTCGTTGTAACAACCACACTGAACTGCTAGGAAACCTGAAGGCGGTGAACCA AGCAATCCAGAGGGCAGGCCGTCTGCGAGTTGGAAAGCCAAAGAACCAGGTGATCAGTGCTTGTCGGGAT GCGATTCGCAGTAACAACATCAACACACTCTTCAGAATCATGCGGGTGGGGACTGCTCCTTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR205555 representing NM_053618
Red=Cloning site Green=Tags(s) MLLPVFTLKLRHKISPRMVAIGRYDGTHPCLAAATQAGKVFIHNPHMRSQHFSTSRVFQSPLESDVSLLN INQTVSCLGAGVLNPELGYDTLLVGTQTSLLAYDIYNNSDLFYREVADGANALVLGTLGDIAPPLAIIGG NCALQGFDHEGNDLFWTVTGDNVHSLALCDFDGDGKSELLVGSEDFDIRVFKEDEIVAEMTETEIVTSLC PMYGSRFGYALSNGTVGVYDKTARYWRIKSKNHAMSIHAFDINSDGVCELITGWSNGKVDARSDRTGEVI FKDNFSSAVAGVVEGDYRMDGHVQLICCSVDGEIRGYLPGTAEMKGNLLDTSVEQGLIRELSQKKQNLLL ELRNYEENTKAELSSPLNEADGQKGIIPANTKLHTALSVNLGNDAQDAHAELRISTSNDTIIRAVLIFAE GIFAGESHVVHPSTHNLSSSIRVPITPPKDVPVDLHLKTFVGYRSSTQFHVFELIRQLPRFTMYALTSPD AASEPVSFVNFIVVERAQRMVTWLNQNFLLPEDSNIQNAPFHVCFTSLRNGGQLYIKMKPSGEITVNTDD IDLAGDIIQSMASFFAIEDLQVEADFPVYFEELRKVLLKVDEYHSVHQKLSANMADNSNLIRSLLVRAED ARLMRDMKTMKTRYMELYDLNKDLLNGYKIRCNNHTELLGNLKAVNQAIQRAGRLRVGKPKNQVISACRD AIRSNNINTLFRIMRVGTAPS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_053618 |
| ORF Size | 2163 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_053618.1, NP_446070.1 |
| RefSeq Size | 2573 bp |
| RefSeq ORF | 2166 bp |
| Locus ID | 113948 |
| UniProt ID | Q99MH9 |
| MW | 79.9 kDa |
| Gene Summary | The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. The BBSome complex, together with the LTZL1, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. Required for proper BBSome complex assembly and its ciliary localization (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN205555 | Bbs2 (untagged ORF) - Rat Bardet-Biedl syndrome 2 homolog (human) (Bbs2), (10 ug) |
CNY 9,220.00 |
|
| RR205555L3 | Lenti ORF clone of Bbs2 (Myc-DDK-tagged ORF) - Rat Bardet-Biedl syndrome 2 homolog (human) (Bbs2), (10 ug) |
CNY 11,020.00 |
|
| RR205555L4 | Lenti ORF clone of Bbs2 (mGFP-tagged ORF) - Rat Bardet-Biedl syndrome 2 homolog (human) (Bbs2), (10 ug) |
CNY 12,160.00 |
