Cela1 (NM_012552) Rat Tagged ORF Clone
CAT#: RR205522
- TrueORF®
Cela1 (Myc-DDK-tagged ORF) - Rat elastase 1, pancreatic (Ela1), (10 ug)
"NM_012552" in other vectors (3)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Ela1; ELAI1; RATELAI1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR205522 representing NM_012552
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCGCTTCCTGGTGTTCGCTTCCCTGGTCCTGTATGGACACAGTACCCAGGACTTTCCGGAAACTA ATGCCCGCGTGGTTGGAGGGGCTGAAGCCCGGAGGAACTCCTGGCCATCTCAGATTTCCCTCCAGTACCT GTCCGGAGGATCATGGTACCACACCTGTGGAGGGACCCTCATCCGACGCAACTGGGTGATGACCGCTGCC CACTGTGTGAGCAGCCAGATGACTTTCCGAGTGGTCGTCGGAGACCACAACCTGAGTCAGAATGACGGCA CGGAGCAGTACGTGAGCGTGCAGAAGATCGTGGTGCACCCCAACTGGAACAGCAACAATGTGGCTGCAGG CTATGACATCGCCCTATTGCGCTTGGCCCAGAGTGTCACACTCAATAACTACGTCCAGCTGGCTGTTTTG CCCCAGGAGGGAACCATCCTGGCTAACAACAATCCCTGCTATATCACAGGCTGGGGGAGAACCAGAACCA ATGGGCAGCTGTCTCAGACCCTGCAGCAGGCGTACCTGCCCAGCGTGGACTACAGCATCTGCTCCAGCTC CTCTTACTGGGGCTCCACGGTGAAGACGACCATGGTGTGTGCTGGTGGAGACGGAGTCCGCTCTGGGTGC CAGGGTGATTCTGGGGGACCCCTCCATTGCTTGGTGAACGGCCAGTATTCTGTCCACGGAGTGACCAGCT TTGTGTCCAGTATGGGCTGTAATGTCTCCAGGAAGCCCACAGTCTTCACCCGAGTCTCTGCTTACATTTC CTGGATGAACAATGTCATTGCCTACAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR205522 representing NM_012552
Red=Cloning site Green=Tags(s) MLRFLVFASLVLYGHSTQDFPETNARVVGGAEARRNSWPSQISLQYLSGGSWYHTCGGTLIRRNWVMTAA HCVSSQMTFRVVVGDHNLSQNDGTEQYVSVQKIVVHPNWNSNNVAAGYDIALLRLAQSVTLNNYVQLAVL PQEGTILANNNPCYITGWGRTRTNGQLSQTLQQAYLPSVDYSICSSSSYWGSTVKTTMVCAGGDGVRSGC QGDSGGPLHCLVNGQYSVHGVTSFVSSMGCNVSRKPTVFTRVSAYISWMNNVIAYN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_012552 |
ORF Size | 798 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_012552.4, NP_036684.1 |
RefSeq Size | 801 bp |
RefSeq ORF | 801 bp |
Locus ID | 24331 |
UniProt ID | P00773 |
MW | 29 kDa |
Gene Summary | catalyzes the hydrolysis of elastin and other proteins [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN205522 | Cela1 (untagged ORF) - Rat elastase 1, pancreatic (Ela1), (10 ug) |
CNY 3,990.00 |
|
RR205522L3 | Lenti ORF clone of Cela1 (Myc-DDK-tagged ORF) - Rat elastase 1, pancreatic (Ela1), (10 ug) |
CNY 6,080.00 |
|
RR205522L4 | Lenti ORF clone of Cela1 (mGFP-tagged ORF) - Rat elastase 1, pancreatic (Ela1), (10 ug) |
CNY 6,650.00 |