Adh7 (NM_134329) Rat Tagged ORF Clone

CAT#: RR205471

  • TrueORF®

Adh7 (Myc-DDK-tagged ORF) - Rat alcohol dehydrogenase 7 (class IV), mu or sigma polypeptide (Adh7), (10 ug)



  "NM_134329" in other vectors (3)

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CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR205471 representing NM_134329
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACACTGCTGGAAAAGTTATTAAGTGCAAAGCAGCTGTCCTATGGGGGACGAACCAGCCCTTCTCCA
TTGAGGACATTGAAGTGGCCCCACCAAAGGCTAAGGAAGTTCGTGTTAAGATTTTGGCCACGGGAATCTG
TGGCACAGATGACCACGTGATCAAGGGAACGATGGTGTCTAAGTTCCCAGTGATTGTGGGACACGAAGCA
GTTGGGATTGTGGAGAGTGTTGGAGAAGAGGTCACTACAGTGAGACCAGGTGACAAAGTGATCCCCCTCT
TTCTACCACAGTGTAGAGAATGCAACCCCTGCCGCAACCCGGAGGAAAATCTCTGCATTAGGAGCGACCT
GACAGGCCGTGGAGTATTGGCTGACGGCACGACCAGGTTCACATGCAAGGGCAAACCAGTCCAGCACTTT
ATGAACACCAGCACCTTCACTGAGTACACGGTCCTGGATGAATCCTCCGTAGCTAAGATTGATGCCGAGG
CTCCTCCCGAGAAAGCCTGCTTGATTGGCTGTGGATTTTCCACTGGTTATGGGGCTGCTGTTAAAACTGC
CAAGGTCAGCCCTGGCTCCACCTGTGCTGTGTTTGGCCTGGGAGGAGTTGGCCTGTCAGTCGTCATGGGC
TGTAAAGCGGCCGGCGCCTCCCGGATCATCGGAATTGACATCAACAAGGACAAATTTCAGAAAGCCCTGG
ATGTAGGGGCCACAGAGTGTATCAATCCCAGGGACTTCACCAAGCCCATCAGTGAGGTGCTGTCAGACAT
GACAGGCAACACTGTCCAGTATACTTTTGAAGTTATCGGGCGCCTTGAGACCATGGTTGATGCCCTCTCA
TCTTGCCATATGAACTATGGGACCAGCGTGGTGGTCGGGGCTCCTCCGTCAGCCAAGATGCTCAGCTATG
ACCCAATGCTGCTTTTCACTGGACGGACATGGAAGGGCTGCGTCTTTGGTGGTTGGAAGAGCAGAGATGA
TGTTCCCAAATTGGTGACTGAATTCCTGGAAAAGAAATTTGACCTGGGCCAGTTGATAACCCACACCTTG
CCTTTTCATAACATCAGTGAAGGATTTGAATTGCTTTATTCAGGGCAAAGCATTCGGACTGTCCTGACAT
TT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR205471 representing NM_134329
Red=Cloning site Green=Tags(s)

MDTAGKVIKCKAAVLWGTNQPFSIEDIEVAPPKAKEVRVKILATGICGTDDHVIKGTMVSKFPVIVGHEA
VGIVESVGEEVTTVRPGDKVIPLFLPQCRECNPCRNPEENLCIRSDLTGRGVLADGTTRFTCKGKPVQHF
MNTSTFTEYTVLDESSVAKIDAEAPPEKACLIGCGFSTGYGAAVKTAKVSPGSTCAVFGLGGVGLSVVMG
CKAAGASRIIGIDINKDKFQKALDVGATECINPRDFTKPISEVLSDMTGNTVQYTFEVIGRLETMVDALS
SCHMNYGTSVVVGAPPSAKMLSYDPMLLFTGRTWKGCVFGGWKSRDDVPKLVTEFLEKKFDLGQLITHTL
PFHNISEGFELLYSGQSIRTVLTF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_134329
ORF Size 1122 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_134329.1, NP_599156.1
RefSeq Size 2048 bp
RefSeq ORF 1125 bp
Locus ID 171178
UniProt ID P41682
MW 40.2 kDa
Gene Summary Catalyzes the NAD-dependent oxidation of all-trans-retinol, alcohol, aldehyde and omega-hydroxy fatty acids and their derivatives. Oxidizes preferentially all trans-retinol, all-trans-4-hydroxyretinol, 9-cis-retinol, 2-hexenol, and long chain omega-hydroxy fatty acids such as juniperic acid. In vitro can also catalyzes the NADH-dependent reduction of all-trans-retinal and aldehydes and their derivatives. Reduces preferentially all trans-retinal, all-trans-4-oxoretinal and hexanal. Catalyzes in the oxidative direction with higher efficiency. Therefore may participate in retinoid metabolism, fatty acid omega-oxidation, and elimination of cytotoxic aldehydes produced by lipid peroxidation.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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