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Rasgrp1 (NM_019211) Rat Tagged ORF Clone
CAT＃: RR205445
- TrueORF®
Rasgrp1 (Myc-DDK-tagged ORF) - Rat RAS guanyl releasing protein 1 (calcium and DAG-regulated) (Rasgrp1), (10 ug)
"NM_019211" in other vectors (3)
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CNY 10,170.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Rasgrp |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR205445 representing NM_019211
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAACCCTGGGCAAGGCGAGAGAGGCTCCGCGGAAACCTTGCCATGGCTCCAGAGCTGGCCCCAAAG GAAGACTAGAGGCCAAATCAACCAACAGTCCTCTCCCTGCCCAGCCCAGCTTGGCCCAGATCACCCAGTT CCGAATGATGGTGTCCCTGGGACATCTGGCCAAAGGAGCCAGCCTGGATGATCTTATTGACAGCTGCATT CAATCTTTCGATGCAGATGGAAACCTGTGTCGAAGTAACCAGCTGTTACAAGTCATGCTAACCATGCACC GAATCATCATCTCCTCGGCCGAGCTGCTGCAAAAACTCATGAATCTATATAAGGACGCCCTGGAAAAGAA TTCTCCAGGAATTTGCTTGAAGATCTGCTATTTTGTCAGGTATTGGATAACAGAATTCTGGATCATGTTC AAAATGGATGCCAGCTTGACCAGCACCATGGAAGAGTTTCAGGACCTGGTGAAAGCCAATGGTGAGGAGT CCCACTGCCACCTCATCGACACGACACAAATTAATTCTCGAGACTGGTCCAGGAAACTGACTCAGAGGAT AAAATCAAATACCAGCAAGAAGCGGAAAGTGTCCCTGCTGTTTGACCATCTTGAACCTGAAGAACTGTCT GAACACCTCACCTACCTTGAGTTCAAGTCCTTCCGACGGATATCTTTCTCTGATTATCAGAATTACCTTG TAAACAGCTGCGTAAAGGAGAACCCCACCATGGAGCGGTCCATTGCCCTGTGCAATGGCATCTCCCAGTG GGTACAACTGATGGTTCTCAGCCGTCCCACCCCACAGCTCCGGGCAGAGGTCTTCATCAAGTTCATCCAT GTGGCTCAGAAGCTCCACCAGCTACAGAACTTCAACACGCTAATGGCTGTGATCGGGGGACTGTGTCACA GCTCCATCTCCAGGCTCAAGGAGACAAGTTCACATGTCCCACATGAGATCAATAAGGTTCTGGGTGAGAT GACTGAACTGCTGTCCTCCTGCAGAAACTATGACAACTACAGGCGAGCCTATGGTGAGTGCACCCACTTC AAAATCCCCATACTGGGTGTGCACCTCAAGGACCTCATATCCCTATATGAAGCCATGCCTGACTACCTGG AAGACGGGAAGGTGAATGTCCAAAAGCTCCTGGCCCTTTACAATCACATCAATGAGTTGGTCCAGCTGCA GGACGTGGCCCCACCATTGGATGCCAACAAGGACCTGGTGCACCTGCTGACGTTATCCCTGGATCTATAC TACACCGAAGATGAAATCTATGAGCTTTCCTACGCCCGTGAACCAAGGAACCACAGGGCCCCGCCACTGA CACCTTCGAAGCCACCAGTTGTAGTGGACTGGGCCTCTGGAGTGTCTCCCAAACCTGACCCGAAGACCAT CAGCAAACACGTCCAAAGGATGGTGGATTCTGTCTTTAAGAACTATGATCTCGACCAGGATGGATATATC TCTCAGGAGGAGTTTGAAAAGATTGCTGCGAGCTTTCCATTTTCCTTCTGTGTGATGGACAAAGATAGGG AGGGCCTCATCAGCAGAGACGAGATCACAGCCTACTTCATGAGGGCCAGCTCCATCTATTCCAAGCTGGG CCTAGGCTTTCCACACAACTTTCAAGAGACCACTTACCTGAAGCCCACCTTCTGTGACAACTGTGCTGGC TTTCTCTGGGGTGTGATCAAGCAAGGCTATCGCTGTAAAGACTGTGGGATGAACTGCCACAAACAGTGCA AAGACCTGGTAGTGTTTGAGTGCAAGAAACGATCCAAGAGCCCGGCGGTATCCACAGAAAACATCAGCTC TGTGGTGCCAATGTCCACTCTTTGTCCACTGGGAACCAAAGATCTGCTCCATGCACCCGAAGAAGGATCT TTCATTTTCCAGAATGGAGAGGTTGTGGACCACAGTGAGGAGAGCAAGGATAGGACCATCATGCTCTTGG GCGTATCCTCACAGAAAATTTCAGTTCGGCTGAAGAGGACTGTTGCCCACAAGACCACCCAGACAGAATC ATTTCCTTGGGTTGGCGGCGAGATGCCCCCTGGTCACTTTGTGCTGACTTCTCCAAGAAAGTCAGCACAA GGGGCTCTTTATGTGCACAGTCCAGCATCTCCGTGCCCCAGCCCAGCACTGGTCCGGAAGCGGGCATTCG TCAAGTGGGAGAACAAAGAGTCCCTTATCAAACCAAAACCAGAGCTTCACCTCAGGCTCCGGACCTACCA AGAACTGGAACAGGAGGTAAATACCCTGAGGGCAGATAACGATGCTCTGAAGATCCAGCTGAAGTATGCA CAGAAACAAATAGAATCCCTGCAGCTTGGCAAAAGCAATCACGTCTTAGCACAGATGGACCACGGTGATG GTACT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR205445 representing NM_019211
Red=Cloning site Green=Tags(s) MGTLGKAREAPRKPCHGSRAGPKGRLEAKSTNSPLPAQPSLAQITQFRMMVSLGHLAKGASLDDLIDSCI QSFDADGNLCRSNQLLQVMLTMHRIIISSAELLQKLMNLYKDALEKNSPGICLKICYFVRYWITEFWIMF KMDASLTSTMEEFQDLVKANGEESHCHLIDTTQINSRDWSRKLTQRIKSNTSKKRKVSLLFDHLEPEELS EHLTYLEFKSFRRISFSDYQNYLVNSCVKENPTMERSIALCNGISQWVQLMVLSRPTPQLRAEVFIKFIH VAQKLHQLQNFNTLMAVIGGLCHSSISRLKETSSHVPHEINKVLGEMTELLSSCRNYDNYRRAYGECTHF KIPILGVHLKDLISLYEAMPDYLEDGKVNVQKLLALYNHINELVQLQDVAPPLDANKDLVHLLTLSLDLY YTEDEIYELSYAREPRNHRAPPLTPSKPPVVVDWASGVSPKPDPKTISKHVQRMVDSVFKNYDLDQDGYI SQEEFEKIAASFPFSFCVMDKDREGLISRDEITAYFMRASSIYSKLGLGFPHNFQETTYLKPTFCDNCAG FLWGVIKQGYRCKDCGMNCHKQCKDLVVFECKKRSKSPAVSTENISSVVPMSTLCPLGTKDLLHAPEEGS FIFQNGEVVDHSEESKDRTIMLLGVSSQKISVRLKRTVAHKTTQTESFPWVGGEMPPGHFVLTSPRKSAQ GALYVHSPASPCPSPALVRKRAFVKWENKESLIKPKPELHLRLRTYQELEQEVNTLRADNDALKIQLKYA QKQIESLQLGKSNHVLAQMDHGDGT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019211 |
| ORF Size | 2385 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019211.2, NP_062084.2 |
| RefSeq Size | 3624 bp |
| RefSeq ORF | 2388 bp |
| Locus ID | 29434 |
| UniProt ID | Q9R1K8 |
| MW | 90.2 kDa |
| Gene Summary | putative guanine nucleotide exchange factor with Ca2+ and diacylglycerol binding domains; may activate Rap protein mediated inhibition of Ras signaling pathways [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN205445 | Rasgrp1 (untagged ORF) - Rat RAS guanyl releasing protein 1 (calcium and DAG-regulated) (Rasgrp1), (10 ug) |
CNY 10,170.00 |
|
| RR205445L3 | Lenti ORF clone of Rasgrp1 (Myc-DDK-tagged ORF) - Rat RAS guanyl releasing protein 1 (calcium and DAG-regulated) (Rasgrp1), (10 ug) |
CNY 12,070.00 |
|
| RR205445L4 | Lenti ORF clone of Rasgrp1 (mGFP-tagged ORF) - Rat RAS guanyl releasing protein 1 (calcium and DAG-regulated) (Rasgrp1), (10 ug) |
CNY 13,210.00 |
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