Tbc1d9b (NM_001108274) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1310147
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR205396 representing NM_001108274 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGCTCGGCCCGGAGGAGGTGCTGGTGGCCAACGCGCTATGGGTGACGGAGCGGGCCAACCCCTTCT TCGTGCTGCAGCGGCGCCGAGGCCATGGCAAGGGTGGCGGCCTCACGGGTCTTCTTGTGGGCACCCTGGA TGTGGTGCTGGACTCCAGTGCCCGTGTGGCCCCCTACCGCATCCTGCACCAGACCCAGGACTCACAGGTC TACTGGACGGTGGCATGTGGTTCTTCCCGCAAAGAGATCACCAAACACTGGGAGTGGCTGGAAAACAACT TACTTCAGACGCTGTCCATCTTCGACAACGAGGAGGACATCACTACCTTCGTCAAGGGCAAGATACACGG CATCATCGCAGAAGAGAACAAGAACCTGCAGCCCCAGGGGGACGAGGACCCAGGGAAATTCAAGGAGGCA GAGCTAAAGATGCGGAAGCAGTTCGGCATGCCAGAGGGAGAGAAGCTGGTCAACTACTACTCCTGTAGCT TCTGGAAGGGCCGAGTGCCTCGGCAGGGCTGGCTCTACCTGACCGTCAACCACCTGTGTTTCTACTCCTT CTTGCTGGGGAAGGAAGTGAGCCTGGTGGTACAGTGGGTGGACGTTACACGCTTGGAGAAGAATGCCACT TTACTCTTCCCCGAGAGCATCCGTGTGGACACGCGGGACCAGGAGCTATTCTTCTCCATGTTCCTAAACA TTGGTGAGACCTTCAAGCTCATGGAACAGCTGGCCAACCTGGCCATGCGACAACTGCTGGACAGCGAGGG CTTCCTGGAGGACAAGGCCCTGCCCAGGCCTATCCGGCCACATAAGAACATCTCAGCTCTAAAGCGAGAC CTGGATGCCCGAGCTAAGAACGAGTGCTACAGGGCCACGTTCCGGCTCCCAAAAGATGAACGGCTGGATG GACACACAGGTTGCACCCTGTGGACACCATTTAACAAGCTACACATCCCCGGCCAGATGTTCATCTCCTA TAACTACATCTGCTTTGCCAGCAAAGAGGAAGATGCGTGCCGCCTCATCATACCCCTGAGGGAGGTGACC ATTGTTGAGAAAGCTGACAGCTCCAGTGTCCTGCCCAGCCCTCTGTCTATCAGCACTAAGAGTAAAATGA CCTTCTTGTTTGCCAACCTGAAAGACCGTGACTTCCTGGTTCAGAGGATCTCTGACTTCCTCCAGAAAAC ACCGTCCAAACAGACAGGCAGCATCGCTGGGGGAACAAAGGCCAGTGTTCCAGACCCAGCCCCAGAGTCT CTCCCAACTCCTCAGGAGGCATCAGAGCCACCCACCAGCCCAACCTCTCCCCTAAGCAGCCCTCTGAGTT TCAGTACACAGGAGATTCCTACTACTTCCCAGGGCCTGCTCAAAGTCTTCCAGAAGAACTCACCCATGGA GGACCTCGGCGCCAAAGGGGCCAAGGAGAAGATGAAGGAAGAGTCCTGGAACATCCACTTCTTTGAGTAC GGGCGTGGCGTGTGCATGTACCGAACAGCCAAAACTCGGGAGCTGGTCCTGAAAGGCATCCCCGAGAGCC TCCGAGGGGAGCTTTGGCTCCTCTTCTCGGGGGCCTGGAATGAGATGGTGACCCATCCTGGCTACTACGC TGAGCTAGTAGAGAAGTCCATGGGGAAGTACAGCTTGGCCACCGAAGAGATCGAGCGGGACCTCCATCGC TCCATGCCTGAGCACCCTGCCTTCCAGAACGAGCTGGGGATCGCTGCACTCCGGCGGGTGCTGACCGCCT ACGCCTTCCGAAACCCGACTATTGGCTACTGCCAGGCAATGAACATCGTGACCTCGGTGCTCCTGCTCTA TGGCAGTGAGGAAGAAGCCTTCTGGCTACTGGTGGCCCTGTGTGAGCGCATGCTGCCCGACTACTACAAT ACCAGAGTAGTGGGGGCCCTCGTAGACCAAGGTATCTTTGAAGAGCTCACAAGAGACGTCCTGCCCCGGC TCTCAGAGAAGATGCAGGAGCTGGGGGTGATCTCCAGCATCTCGCTGTCCTGGTTCCTGACCCTCTTCCT CAGCGTCATGCCCTTTGAGAGCGCAGTGGTTATTGTTGACTGCTTTTTCTACGAGGGCATCAAAGTGATA CTGCAGGTGGCCTTGGCTGTGCTGGACGCCAACATGGAACAGCTGCTGGACTGCAGTGACGAGGGCGAGG CCATGACCGTGCTGGGCAGGTACCTGGACAATGTGGTCAACAAGCAGAGCATTTCTCCACCTATCCCACA CCTCCATGCTCTGCTGACCAGTGGAGATGACCCTCCTGCAGAGGTGGACATCTTTGACCTCCTGAAAGTG TCTTATGAGAAGTTCAGCAGCCTGAGGGCTGACGACATTGAACAGATGCGGTTTAAACAGAGGCTGAAGG TGATCCAGTCCTTGGAAGACACAGCCAAGAGAAGCGTGGTCCGGGCCATACCAGGGGACATTGGCTTCTC CATCGAAGAGCTGGAGGATCTTTACATGGTGTTTAAGGCCAAGCATCTGGCAAGCCAGTACTGGGGTGGT AACCGCTCAGCAGCCATCCACCGAGACCCCAGCCTGCCCTACCTGGAGCAGTACCGGATCGATGCCAGCC AGTTTCGGGAGCTCTTTGCCAGCCTGACGCCTTGGGCCTGTGGCTCTCACACGCCTGTCTTGGCAGGCCG CATGTTCCGCCTCCTGGACCAAAACAAGGACTCACTGATCAACTTCAAGGAGTTTGTGACAGGGATGAGT GGGATGTACCATGGAGACCTCACAGAGAAGCTCAAAGCGCTCTACAAGCTGCACCTGCCCCCAGCTCTGA TCCCAGAGGAAGCGGAGTCAGCCCTGGAGGCCGCCCATTATTTCCCAGAGGATAGCTCCTCAGAAGCATC TCCTCTGGCCTCAGATCTGGATCTTTTCCTGCCTTGGGAGGCTCAAGGACTGCAGGAGCAGCAGGAAGGA AGAGGAAGTGAGGACATCCAGGAAAGGAGAGAGGAGAAGGAGACCAGCCCCCCTGACTACCGACACTACC TTCGAATATGGGCTAAGGAGAAAGAGGCTCAGAAGGAAACCATCAAGGACCTTCCCAAGATGAACCAGGA GCAATTCATTGAGCTGTGTAAAACACTTTACAACATGTTTAGCGAAGACCCTTTGGAACAGGACTTGTAC CATGCCATCGCCACTGTGGCCAGCCTTCTCCTCCGCATTGGTGAGGTGGGGAAGAAGTTTTCGGCCCTGA CCACCAAGAAGCCCAGGGATGGTGCCCACAGTGGGGATCACAACAGTGCCACAGAAGAGGATGAGCCACC CATATCCAAACTCCATCAGGACCCAGCACGGGAATGCCAGCCACCAGCTGCAGGGGATCCGCAGGCCAAA GCCAGTGGCGACATGCATCTTGGGAAAGCATTGCAGGACAGTCACGTGATAGTGGAGGGAGGCAGCGGCG AGGGGCAGGGCTCTCCTTCCCTGCTTTTGTCTGATGATGAAACCAAAGATGACATGTCCATGTCCTCCTA CTCAGTAGTCAGCACGGGGTCCCTGCCGTGCGAGGACCTCACAGAAGACACAGTGCTGGTGGGAGGAGAA GCCTGCAGCCCCACTGCCACCTCACGGATGGGAGGCACCGTGGACATGGATTGGTGCATTTCCTTCGAGC AGATCCTGGCCTCCATCCTGACAGAGTCTGTGCTAGTGAACTTCTTTGAGAAGAGGGTAGACATTGGACT CAAGATCAAAGACCAGAAGAAAGTCGAAAGGCAGTTCAGCACCTCCAGTGACCATGAGCCCCCTGGGGTC TTGGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR205396 representing NM_001108274 Red=Cloning site Green=Tags(s) MWLGPEEVLVANALWVTERANPFFVLQRRRGHGKGGGLTGLLVGTLDVVLDSSARVAPYRILHQTQDSQV YWTVACGSSRKEITKHWEWLENNLLQTLSIFDNEEDITTFVKGKIHGIIAEENKNLQPQGDEDPGKFKEA ELKMRKQFGMPEGEKLVNYYSCSFWKGRVPRQGWLYLTVNHLCFYSFLLGKEVSLVVQWVDVTRLEKNAT LLFPESIRVDTRDQELFFSMFLNIGETFKLMEQLANLAMRQLLDSEGFLEDKALPRPIRPHKNISALKRD LDARAKNECYRATFRLPKDERLDGHTGCTLWTPFNKLHIPGQMFISYNYICFASKEEDACRLIIPLREVT IVEKADSSSVLPSPLSISTKSKMTFLFANLKDRDFLVQRISDFLQKTPSKQTGSIAGGTKASVPDPAPES LPTPQEASEPPTSPTSPLSSPLSFSTQEIPTTSQGLLKVFQKNSPMEDLGAKGAKEKMKEESWNIHFFEY GRGVCMYRTAKTRELVLKGIPESLRGELWLLFSGAWNEMVTHPGYYAELVEKSMGKYSLATEEIERDLHR SMPEHPAFQNELGIAALRRVLTAYAFRNPTIGYCQAMNIVTSVLLLYGSEEEAFWLLVALCERMLPDYYN TRVVGALVDQGIFEELTRDVLPRLSEKMQELGVISSISLSWFLTLFLSVMPFESAVVIVDCFFYEGIKVI LQVALAVLDANMEQLLDCSDEGEAMTVLGRYLDNVVNKQSISPPIPHLHALLTSGDDPPAEVDIFDLLKV SYEKFSSLRADDIEQMRFKQRLKVIQSLEDTAKRSVVRAIPGDIGFSIEELEDLYMVFKAKHLASQYWGG NRSAAIHRDPSLPYLEQYRIDASQFRELFASLTPWACGSHTPVLAGRMFRLLDQNKDSLINFKEFVTGMS GMYHGDLTEKLKALYKLHLPPALIPEEAESALEAAHYFPEDSSSEASPLASDLDLFLPWEAQGLQEQQEG RGSEDIQERREEKETSPPDYRHYLRIWAKEKEAQKETIKDLPKMNQEQFIELCKTLYNMFSEDPLEQDLY HAIATVASLLLRIGEVGKKFSALTTKKPRDGAHSGDHNSATEEDEPPISKLHQDPARECQPPAAGDPQAK ASGDMHLGKALQDSHVIVEGGSGEGQGSPSLLLSDDETKDDMSMSSYSVVSTGSLPCEDLTEDTVLVGGE ACSPTATSRMGGTVDMDWCISFEQILASILTESVLVNFFEKRVDIGLKIKDQKKVERQFSTSSDHEPPGV LG TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001108274
ORF Size	3786 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001108274.1, NP_001101744.1
RefSeq Size	5651 bp
RefSeq ORF	3789 bp
Locus ID	360520
MW	141.9 kDa

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