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Dnaaf1 (NM_001014154) Rat Tagged ORF Clone
CAT＃: RR205317
- TrueORF®
Dnaaf1 (Myc-DDK-tagged ORF) - Rat leucine rich repeat containing 50 (Lrrc50), (10 ug)
"NM_001014154" in other vectors (3)
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CNY 8,080.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Lrrc50; RGD1310542 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR205317 representing NM_001014154
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACCCCGAAGCCTCAGAGCCGCCGGTGGACAGCGCGGCGGAGCCCAGTCTGGAGGAGTCCGCAGGCG ATCACGGCGACGCAGGCCCAGGGGTCCGCAAAGAAGAAATTAATGAGACCAAGGAAACATGTGTGGGTCC TTGTACAACATCCTGCCAAAGTCAGCAGCAACCGAGCGGTGACAATGGGTCAGATGGCCTCTTTACCCAC TCGAGAGATGACAGAGATGATCGGGGCCCCAGGATGACGAAGCAGTTCCTCCAAAAGCTCTGTAAGCAGC ACAAGCTGTACGTCACCCCGGCCCTGAACGACACCCTGTATTTGCACTTTAAGGGCTTTGACCGCATTGA GAACCTGGAGGAGTACACGGGCCTGCGCTGCCTCTGGCTGGAGTGCAACGGCATCCAGAGGATCGAGAAT TTGCAGGCCCAGAGCGAGCTGCGCTGCCTCTTCCTGCAAGTGAACCTGTTGCACAAGATCGAAAACCTGG AGCCGCTCCAGAAGCTGGACGCCCTCAACCTGAGCAACAACTACATCAAGACCATTGAGAACCTCTCCTG TCTGCCGGTCCTTAACACTCTGCAAATGGCCCACAACCGCCTGGAGACCGTGGCAGACATTGAGCACCTC AGGGAGTGTCTGCAACTCTGTGTCCTCGACCTCTCCCACAACTCGCTGAGTGACCCCGAGATCCTGAGCG TGCTTGAGACCATGCCCTGTCTGCGTGTGCTGAACCTGATGGGGAACCCAGTGACCAAACACATCCCTAA CTACCGCAGGACGGTCACTGTCCGACTGAAGCACTTGACTTATCTGGACGACAGGCCGGTTTTCCCCAAG GACAGGGCGTGTGCAGAGGCCTGGGCCCGAGGCGGGTATGCTGCCGAGAAGGAAGAGCGTCACCAGTGGG AGAGCAGGGAACACAAGAAGATAACAGACAGCCTGGAAGCCTTGGCCATGATCAAGCGGCGGGCCGAGGA ACGGAAGAAGGCAAGAGACAGAGGGGAGACACCTCTGCCAGAGAGTGAAAAGAGTATCCCCACTAGCCCA GAGGCTCAGGAAAAGCCTCCCAAGGGGGAAACCCAGCAGAAGATGGAGTCGTTTGTGAAAGAGAGCTTTG AGGCCAAAGATGAACTGTTTCCAGAGAAGCCAGGAGAAGGAGAGGAGCTGTCTGTGGTGGTGGGGAATAG AGCAGTGGAAGATGCAGACCTGTCCGGGAACCTGGCTCACACCCAGACACCTGTGGTGGTCACCCCTGAA GAGGTCACATCTCCAGTGGAAGCTACTGATGGGGCCAGAACGGAAGACACAGAGGCCATTGCCCTGGAGA CCAAGGAGAAGCTTTTCATCGATGACCTGCCTGACTTGGAAGATGTTGATGGTACAGATGTGTCTGTGGA AGACCAGACAAAGGACACAGGCATCCGGAAAATCCAGGCCATCTCCAGCTTGAGTGACGACAGTGACCTG GAGCTGGAGGAGCTGCCCCTCTCCGTGTTCGAGGGCACTCCCATAAGCCCCACAGGCGCCCTCTCACACA TATTTGCGGTCTCCAAAGACCCCTCAGAGGCAGCCAGGGTGCCTTTCGCAGACATATGTATGCCAACAGC AACAACAGACTTAGAAACTCAAAGTCAAGACCCCAGCACAGCATCTTCACACCCCCTCATCCAGGAGCTC GGAGAGGATGAGCTGACAGAGGGAGAGTCAAACCAGCCACTGCCACCCCAAAGCTGTGCAAGTGACCCCA CACTTGCCCAGTCTAGTGAAGGTGGAGACAGTCAGCTGCCTGCAGCCACTCCACTAGGAGATGGAGCTGA GAATGAAGCACAGTCTTCGTTGTACCCAGAGGAGCCCAGCACCCGGATAGGCCTGGAAGATATTGAATTT GGCTTGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR205317 representing NM_001014154
Red=Cloning site Green=Tags(s) MHPEASEPPVDSAAEPSLEESAGDHGDAGPGVRKEEINETKETCVGPCTTSCQSQQQPSGDNGSDGLFTH SRDDRDDRGPRMTKQFLQKLCKQHKLYVTPALNDTLYLHFKGFDRIENLEEYTGLRCLWLECNGIQRIEN LQAQSELRCLFLQVNLLHKIENLEPLQKLDALNLSNNYIKTIENLSCLPVLNTLQMAHNRLETVADIEHL RECLQLCVLDLSHNSLSDPEILSVLETMPCLRVLNLMGNPVTKHIPNYRRTVTVRLKHLTYLDDRPVFPK DRACAEAWARGGYAAEKEERHQWESREHKKITDSLEALAMIKRRAEERKKARDRGETPLPESEKSIPTSP EAQEKPPKGETQQKMESFVKESFEAKDELFPEKPGEGEELSVVVGNRAVEDADLSGNLAHTQTPVVVTPE EVTSPVEATDGARTEDTEAIALETKEKLFIDDLPDLEDVDGTDVSVEDQTKDTGIRKIQAISSLSDDSDL ELEELPLSVFEGTPISPTGALSHIFAVSKDPSEAARVPFADICMPTATTDLETQSQDPSTASSHPLIQEL GEDELTEGESNQPLPPQSCASDPTLAQSSEGGDSQLPAATPLGDGAENEAQSSLYPEEPSTRIGLEDIEF GLD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001014154 |
| ORF Size | 1899 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001014154.1, NP_001014176.1 |
| RefSeq Size | 2204 bp |
| RefSeq ORF | 1902 bp |
| Locus ID | 361419 |
| UniProt ID | Q6AYH9 |
| MW | 70 kDa |
| Gene Summary | Cilium-specific protein required for the stability of the ciliary architecture. Plays a role in cytoplasmic preassembly of dynein arms (By similarity). Involved in regulation of microtubule-based cilia and actin-based brush border microvilli (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN205317 | Dnaaf1 (untagged ORF) - Rat leucine rich repeat containing 50 (Lrrc50), (10 ug) |
CNY 8,080.00 |
|
| RR205317L3 | Lenti ORF clone of Dnaaf1 (Myc-DDK-tagged ORF) - Rat leucine rich repeat containing 50 (Lrrc50), (10 ug) |
CNY 9,980.00 |
|
| RR205317L4 | Lenti ORF clone of Dnaaf1 (mGFP-tagged ORF) - Rat leucine rich repeat containing 50 (Lrrc50), (10 ug) |
CNY 10,830.00 |
