Hsd3b1 (NM_001007719) Rat Tagged ORF Clone
CAT#: RR205099
- TrueORF®
Hsd3b1 (Myc-DDK-tagged ORF) - Rat hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 (Hsd3b1), (10 ug)
"NM_001007719" in other vectors (3)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | MGC105549 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR205099 representing NM_001007719
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTGGATGGAGCTGCCTGGTGACAGGAGCAGGAGGGTTTGTGGGCCAGAGGATCATCCGGATGTTGG TGCAGGAGAAAGAACTGCAGGAGGTCAGGGCTCTGGACAAAGTCTTCAGACCAGAAACCAAGGAGGAATT CTCTAAGCTGCAGACAAAGGCCAAGGTGACAATGTTAGAAGGAGATATTCTGGATGCCCAGTACCTGAGG AGAGCGTGCCAGGGCATCTCTGTTGTCATCCACACCGCTGCTGTCATTGATGTCTCACATGTCCTACCCA GGCAGACCATCCTAGATGTCAATCTGAAAGGTACCCAGAACATATTGGAGGCCTGCGTCGAAGCCAGTGT GCCAGCCTTCATCTACTGCAGCACAGTTGACGTTGCAGGGCCCAACTCCTACAAGAAGATCATCCTGAAT GGCCATGAGGAAGAGCATCATGAAAGCACATGGTCAGATGCATACCCATACAGCAAAAGGATGGCCGAGA AGGCAGTGCTGGCAGCCAATGGGAGCATCCTGAAAAATGGTGGCACACTGCATACTTGTGCCTTAAGGCC CATGTACATTTATGGGGAGAGAAGTCCATTCCTTTCTGTCATGATACTTGCGGCCCTCAAAAATAAGGGT ATTCTGAATGTTACTGGCAAATTCTCCATAGCCAATCCAGTGTATGTAGGCAATGTGGCCTGGGCACATA TTCTGGCTGCCAGGGGCCTTCGAGACCCCAAGAAGTCACAAAATGTCCAAGGACAGTTCTACTACATCTC AGATGACACCCCTCACCAAAGCTATGATGATTTAAATTGCACCCTGAGCAAGGAATGGGGCCTCCGCCTT GATTCCAGCTGGAGCCTTCCTCTGCCCCTGCTCTACTGGCTTGCCTTCCTGCTGGAAACTGTGAGCTTCC TGCTGCGTCCATTTTACAACTATAGGCCACCCTTTAACTGCCACTTGGTCACACTGTCAAATAGCAAGTT CACTTTCTCCTACAAGAAAGCTCAGAGAGATCTGGGCTATGTGCCACTTGTCAGCTGGGAGGAAGCCAAG CAGAAAACCTCGGAGTGGATCGGGACACTAGTGGAGCAGCACAGGGAGACACTGGACACAAAGTCTCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR205099 representing NM_001007719
Red=Cloning site Green=Tags(s) MPGWSCLVTGAGGFVGQRIIRMLVQEKELQEVRALDKVFRPETKEEFSKLQTKAKVTMLEGDILDAQYLR RACQGISVVIHTAAVIDVSHVLPRQTILDVNLKGTQNILEACVEASVPAFIYCSTVDVAGPNSYKKIILN GHEEEHHESTWSDAYPYSKRMAEKAVLAANGSILKNGGTLHTCALRPMYIYGERSPFLSVMILAALKNKG ILNVTGKFSIANPVYVGNVAWAHILAARGLRDPKKSQNVQGQFYYISDDTPHQSYDDLNCTLSKEWGLRL DSSWSLPLPLLYWLAFLLETVSFLLRPFYNYRPPFNCHLVTLSNSKFTFSYKKAQRDLGYVPLVSWEEAK QKTSEWIGTLVEQHRETLDTKSQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001007719 |
ORF Size | 1119 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001007719.3, NP_001007720.3 |
RefSeq Size | 1640 bp |
RefSeq ORF | 1122 bp |
Locus ID | 360348 |
UniProt ID | P22071 |
MW | 42 kDa |
Gene Summary | A bifunctional enzyme responsible for the oxidation and isomerization of 3beta-hydroxy-Delta(5)-steroid precursors to 3-oxo-Delta(4)-steroids, an essential step in steroid hormone biosynthesis. Specifically catalyzes the conversion of pregnenolone to progesterone, dehydroepiandrosterone (DHEA) to 4-androstenedione, and androstenediol to testosterone (PubMed:1537836, PubMed:1985917). Additionally, catalyzes the interconversion between 3beta-hydroxy and 3-oxo-5alpha-androstane steroids controlling the bioavalability of the active forms. Specifically converts dihydrotestosterone to its inactive form 5alpha-androstanediol, that does not bind androgen receptor/AR. Also converts androstanedione, a precursor of testosterone and estrone, to epiandrosterone (PubMed:1537836). Expected to use NAD(+) as preferred electron donor for the 3beta-hydroxy-steroid dehydrogenase activity and NADPH for the 3-ketosteroid reductase activity (Probable).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN205099 | Hsd3b1 (untagged ORF) - Rat hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 (Hsd3b1), (10 ug) |
CNY 3,990.00 |
|
RR205099L3 | Lenti ORF clone of Hsd3b1 (Myc-DDK-tagged ORF) - Rat hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 (Hsd3b1), (10 ug) |
CNY 6,080.00 |
|
RR205099L4 | Lenti ORF clone of Hsd3b1 (mGFP-tagged ORF) - Rat hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 (Hsd3b1), (10 ug) |
CNY 6,650.00 |