Kdr (NM_013062) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Vegfr-2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR204835 representing NM_013062 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAGCAGGGCGCTGCTAGCTGTCGCTCTGTGGTTCTGCGTGGAGACCCGAGCCGCCTCTGTGGGTT TGCCTGGCGATTCCCTCCATCCACCCAAGCTCAGCACACAAAAAGACATACTTACAATTTTGGCAAATAC AACCCTTCAGATTACTTGCAGGGGACAGAGGGACCTGGATTGGCTTTGGCCCAACACTCCGCGTGACTCT GAGGAAAGGGTGTTGGTGACTGAGTGTGGCGACAGTATCTTCTGCAAGACACTCACAGTTCCCAGAGTGG TTGGAAATGATACTGGAGCCTACAAGTGCTTCTATCGGGACACCGATGTCTCCTCCATCGTTTATGTCTA TGTTCAAGATCACAGGTCACCATTCATCGCCTCTGTCAGTGACGAGCATGGCATCGTGTACATCACTGAG AACAAGAACAAAACTGTGGTGATCCCATGCCGAGGGTCGATTTCAAACCTCAACGTGTCACTTTGTGCTA GGTATCCAGAAAAGAGATTTGTTCCGGATGGAAACAGAATTTCCTGGGACAGCGAGAAAGGCTTTACTAT CCCCAGTTACATGATCAGCTATGCCGGCATGGTCTTCTGTGAGGCAAAGATTAATGATGAAACGTATCAG TCTATCATGTACATAGTTCTGGTTGTAGGATATAGGATTTATGATGTGGTCCTGAGCCCCCCTCATGAAA TTGAGCTATCTGCCGGAGAAAAGCTTGTCTTAAATTGTACAGCAAGAACAGAGCTCAACGTGGGGCTTGA TTTCAGCTGGCAATTCCCGTCCTCAAAGCATCAGCATAAGAAGATTGTAAACCGGGATGTGAAATCCCTT CCTGGGACTGTGGCAAAGATGTTTTTGAGCACCTTGACCATAGACAGTGTGACCAAGAGTGACCAAGGAG AATACACCTGCACAGCGTACAGTGGACTGATGACCAAGAAAAATAAAACATTTGTCCGAGTTCATACAAA ACCTTTTATTGCTTTTGGTAGCGGGATGAAATCTTTGGTGGAAGCCACTGTGGGCAGCCAAGTCCGAATC CCTGTGAAGTATCTCAGTTACCCAGCTCCTGATATCAAATGGTACAGAAATGGACGACCCATTGAGTCCA ATTACACAATGATCGTTGGTGATGAACTCACCATCATGGAAGTGAGTGAAAGAGATGCGGGAAACTACAC GGTCATCCTCACCAATCCCATTTCAATGGAGAAACAGAGCCACATGGTCTCTCTGGTTGTGAATGTTCCA CCCCAGATCGGTGAGAAAGCCTTGATCTCTCCTATGGATTCCTACCAGTATGGCACCATGCAGACGCTGA CATGCACAGTCTATGCCAACCCTCCCCTGCACCACATCCAATGGTACTGGCAGCTAGAAGAAGCATGCTC CTACAGGCCCAGCCAAACAAACCCATATACTTGTAAAGAATGGAGACACGTGAAGGATTTCCAGGGGGGA AATAAGATCGAAGTCACCAAAAACCAATATGCCCTAATTGAAGGAAAAAACAAAACTGTAAGTACTCTGG TCATCCAGGCTGCCTACGTGTCCGCATTATACAAATGTGAAGCCATCAACAAAGCAGGACGAGGAGAGAG GGTCATCTCCTTCCATGTGATCAGGGGTCCTGAAATTACTGTCCAGCCTGCTACCCAGCCAACCGAGCGG GAGAGTATGTCTTTATTGTGCACTGCAGATAGAAACACGTTTGAGAACCTCACGTGGTACAAGCTTGGCT CACAGGCAACATCGGTCCACATGGGCGAATCACTCACACCAGTTTGCAAGAACTTGGACGCTCTTTGGAA ACTGAATGGCACCGTGTTTTCTAACAGCACAAACGACATCTTGATTGTGGCATTCCAGAATGCCTCCCTG CAGGACCAAGGCAACTATGTCTGCTCTGCTCAAGACAAGAAGACCAAGAAAAGACATTGCCTAGTCAAGC AGCTCGTCATCCTAGAGCGCATGGCACCCATGATCACTGGAAATCTGGAGAATCAGACAACAACCATTGG TGAGACCATCGAAGTTGTTTGTCCAACATCTGGAAACCCTACCCCCCTCATTACATGGTTCAAAGACAAT GAGACCCTTGTAGAAGATTCAGGCATTGTACTAAAAGACGGGAACCGGAACCTAACTATCCGAAGGGTGA GGAAGGAAGACGGGGGCCTCTACACCTGCCAGGCCTGCAATGTCCTTGGCTGTGCAAGAGCAGAGACACT CTTCATAATAGAAGGCGTCCAGGAAAAGACCAACTTGGAAGTCATTATCCTCGTCGGCACTGCAGTGATT GCCATGTTCTTCTGGCTCCTTCTTGTCATTCTCGTACGGACCGTTAAGCGGGCCAATGAAGGGGAACTGA AGACAGGCTACTTGTCCATTGTCATGGATCCAGATGAACTGCCCTTGGATGAGCGCTGTGAACGCTTGCC TTATGATGCCAGCAAGTGGGAGTTCCCCAGGGACCGGCTGAAACTAGGAAAACCTCTTGGCCGTGGTGCC TTTGGCCAAGTGATTGAGGCAGATGCCTTTGGAATCGACAAGACAGCGACTTGCAAAACAGTGGCTGTCA AGATGTTGAAAGAGGGAGCAACACACAGCGAGCACCGAGCCCTCATGTCCGAACTCAAGATCCTCATCCA CATTGGCCACCATCTCAATGTGGTGAACCTGCTGGGTGCCTGCACGAAGCCCGGAGGGCCTCTCATGGTG ATTGTAGAATTCTGCAAGTTTGGAAACCTATCAACTTACTTACGGGGCAAGAGAAATGAATTCGTGCCCT ATAAGAGCAAAGGGGCACGCTTCCGCTCTGGGAAAGACTATGTTGGGGAGCTCTCCGTAGACCTGAAGCG GCGCTTGGACAGCATCACCAGCAGTCAGAGCTCTGCCAGCTCAGGTTTTGTGGAGGAGAAATCCCTCAGT GACGTAGAGGAAGAAGAAGCTTCTGAAGAACTGTACAAGGACTTCCTGACCTTGGAGCATCTCATCTGTT ACAGCTTCCAAGTGGCTAAGGGCATGGAGTTCTTGGCATCAAGGAAGTGTATCCACAGGGACCTGGCAGC ACGAAACATTCTCCTATCGGAGAAGAACGTGGTTAAAATCTGTGACTTTGGCTTGGCCCGGGACATTTAT AAAGACCCAGATTACGTCAGAAAAGGAGATCCCCGACTCCCTTTGAAGTGGATGGCTCCGGAAACAATTT TTGACAGAATATACACAATTCAGAGTGGTGTGTGGTCTTTTGGTGTTTTGCTCTGGGAAATATTTTCCTT AGGTGCTTCCCCATATCCTGGGGTCAAGATTGATGAAAAATTTTGTAGGAGATTGAAAGAAGGAACGAGA ATGCGGGCTCCTGACTACACCACCCCAGAAATGTACCAAACCATGCTGGATTGCTGGCATGAGGACCCCA ACCAGAGACCCGCGTTTTCAGAGTTGGTGGAGCACTTGGGAAATCTCCTGCAAGCAAATGCTCAGCAGGA TGGCAAAGACTATATTGTTCTTCCAATGTCAGAGACACTGAGCATGGAAGAGGATTCTGGACTCTCCCTG CCTACCTCACCTGTTTCCTGTATGGAGGAAGAGGAAGTGTGCGACCCCAAATTCCATTATGACAACACAG CAGGAATCAGTCATTATCTGCAGAACAGCAAGCGAAAAAGCCGGCCAGTGAGTGTAAAAACATTTGAAGA TATCCCTTTGGAGGAACCAGAAGTAAAAGTGATTCCAGATGACAGCCAGACAGACAGTGGGATGGTCCTT GCCTCAGAAGAGCTGAAAACTCTGGAAGACAGGAACAAATTATCTCCATCTTTTGGTGGGATGATGCCCA GTAAAAGCAGGGAGTCTGTGGCCTCGGAAGGCTCCAACCAGACCAGCGGCTACCAGTCTGGGTATCACTC AGACGACACAGATACCACCGTGTACTCCAGCGACGAGGCAGGACTTTTAAAGCTGGTGGATGTTGCAGGG CACGTTGACTCTGGGACCACACTGCGCTCATCTCCTGTTCACGTTGACTCTGGGACCACACTGCGCTCAT CTCCTGTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR204835 representing NM_013062 Red=Cloning site Green=Tags(s) MESRALLAVALWFCVETRAASVGLPGDSLHPPKLSTQKDILTILANTTLQITCRGQRDLDWLWPNTPRDS EERVLVTECGDSIFCKTLTVPRVVGNDTGAYKCFYRDTDVSSIVYVYVQDHRSPFIASVSDEHGIVYITE NKNKTVVIPCRGSISNLNVSLCARYPEKRFVPDGNRISWDSEKGFTIPSYMISYAGMVFCEAKINDETYQ SIMYIVLVVGYRIYDVVLSPPHEIELSAGEKLVLNCTARTELNVGLDFSWQFPSSKHQHKKIVNRDVKSL PGTVAKMFLSTLTIDSVTKSDQGEYTCTAYSGLMTKKNKTFVRVHTKPFIAFGSGMKSLVEATVGSQVRI PVKYLSYPAPDIKWYRNGRPIESNYTMIVGDELTIMEVSERDAGNYTVILTNPISMEKQSHMVSLVVNVP PQIGEKALISPMDSYQYGTMQTLTCTVYANPPLHHIQWYWQLEEACSYRPSQTNPYTCKEWRHVKDFQGG NKIEVTKNQYALIEGKNKTVSTLVIQAAYVSALYKCEAINKAGRGERVISFHVIRGPEITVQPATQPTER ESMSLLCTADRNTFENLTWYKLGSQATSVHMGESLTPVCKNLDALWKLNGTVFSNSTNDILIVAFQNASL QDQGNYVCSAQDKKTKKRHCLVKQLVILERMAPMITGNLENQTTTIGETIEVVCPTSGNPTPLITWFKDN ETLVEDSGIVLKDGNRNLTIRRVRKEDGGLYTCQACNVLGCARAETLFIIEGVQEKTNLEVIILVGTAVI AMFFWLLLVILVRTVKRANEGELKTGYLSIVMDPDELPLDERCERLPYDASKWEFPRDRLKLGKPLGRGA FGQVIEADAFGIDKTATCKTVAVKMLKEGATHSEHRALMSELKILIHIGHHLNVVNLLGACTKPGGPLMV IVEFCKFGNLSTYLRGKRNEFVPYKSKGARFRSGKDYVGELSVDLKRRLDSITSSQSSASSGFVEEKSLS DVEEEEASEELYKDFLTLEHLICYSFQVAKGMEFLASRKCIHRDLAARNILLSEKNVVKICDFGLARDIY KDPDYVRKGDPRLPLKWMAPETIFDRIYTIQSGVWSFGVLLWEIFSLGASPYPGVKIDEKFCRRLKEGTR MRAPDYTTPEMYQTMLDCWHEDPNQRPAFSELVEHLGNLLQANAQQDGKDYIVLPMSETLSMEEDSGLSL PTSPVSCMEEEEVCDPKFHYDNTAGISHYLQNSKRKSRPVSVKTFEDIPLEEPEVKVIPDDSQTDSGMVL ASEELKTLEDRNKLSPSFGGMMPSKSRESVASEGSNQTSGYQSGYHSDDTDTTVYSSDEAGLLKLVDVAG HVDSGTTLRSSPVHVDSGTTLRSSPV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_013062
ORF Size	4029 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq Size	5892 bp
RefSeq ORF	4032 bp
Locus ID	25589
UniProt ID	O08775
MW	150.4 kDa
Gene Summary	receptor for vascular endothelial growth factor (VEGF) [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.