Rgs14 (NM_053764) Rat Tagged ORF Clone
CAT#: RR204743
- TrueORF®
Rgs14 (Myc-DDK-tagged ORF) - Rat regulator of G-protein signaling 14 (Rgs14), (10 ug)
"NM_053764" in other vectors (3)
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CNY 6,940.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | MGC108631 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR204743 representing NM_053764
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGGGAAGCCCAAGCACTTGGGTGTCCCCAACGGGCGCATGGTCCTGGCTGTCTCTGATGGAGAAC TGACCAGCACATCAGGTTCCCAGGCCCAGGGTGAGGGCAGAGGCAGCTCCCTCAGCATCCACAGCCTCCC CAGTGGCCCCAGCAGCCCCTTCTCCACTGATGAGCAGCCTGTGGCCAGCTGGGCCCAGTCCTTTGAGCGG CTGCTGCAAGACCCACGGGGTCTGGCTTACTTCACTGAGTTCCTGAAGAAGGAATTCAGCGCCGAGAATG TAACTTTCTGGCAAGCCTGCGAGCGTTTCCAACAGATCCCAGCCAGCGACACCAAACAGCTAGCTCAGGA GGCCCACAACATCTACCATGAGTTCCTGTCCAGCCAGGCACTGAGCCCAGTGAACATCGACCGACAGGCC TGGCTTAGTGAGGAGGTGCTGGCCCAGCCCAGGCCCGATATGTTCCGAGCACAGCAGCTTCAGATCTTCA ATTTGATGAAGTTCGACAGCTACGCGCGCTTCGTCAAATCCCCGCTGTACCAAGAGTGCCTGCTGGCCGA GGCCGAGGGACGCCCCCTGCGGGAACCTGGATCCTCACACCTCGGGAGCCCGGACACTGCGAGGAAGAAG CCAAAGCTGAAGCCTGGAAAGTCACTGCCGCTCGGTGTGGAAGAGTTGGGGCAGCTGCCGCTTGCTGAGG GCCGCCCTCTCCGCAAGTCCTTTCGTAGAGAGATGCCGGGTGGAGCTGTGAATTCGGCCCTGCGACGAGA GTCTCAGGGATCCCTGAATTCTTCCGCGAGTCTGGACCTGGGCTTCCTTGCCTTTGTGAGCAGCAAATCC GAGAGCCACCGAAAGAGCCTTGGAAGTGGAGAGGGTGAGAGCGAAAGCCGGCCCGGGAAGTACTGCTGCG TGTATCTACCTGATGGCACGGCCTCCTTGGCCCTGGCTCGACCTGGCCTCACCATCCGTGACATGCTGGC AGGCATCTGTGAGAAGAGAGGCCTCTCTCTACCTGACATTAAGGTCTACCTGGTGGGCAAAGAACAGAAG GCCCTGGTCCTGGATCAGGACTGCACCGTGCTGGCAGACCAGGAAGTGCGACTGGAAAACAGGATCACCT TCCAGCTGGAGTTGGTCGGCCTGGAGCGTGTGGTACGGATCTCTGCTAAGCCCACCAAGCGTCTGCAGGA GGCGCTGCAGCCTATCTTGGCCAAGCACGGCCTGAGCCTGGATCAGGTGGTCCTACACAGGCCAGGAGAG AAGCAGCTAGTGGATTTGGAGAATCTAGTGAGCTCAGTGGCTTCACAGACACTGGTTTTGGACACTCTTC CAGATGCAAAGACAAGGGAAGCCAGCAGCATACCTCCCTGCCGCAGCCAGGGATGCCTCCCTAGAACCCA GACCAAGGACAGTCACCTTCCTCCACTGTCCTCCAGTTTGTCGGTAGAAGATGCCAGTGGTTCTACTGGG AAACGACAGACCTGCGACATTGAAGGCCTAGTGGAGCTGCTGAATCGTGTGCAGAGCAGCGGGGCCCATG ACCAGAGGGGACTTCTTCGCAAAGAGGACCTGGTCCTTCCAGAATTTCTGCAGCTTCCTTCCCAAAGACC AGGTTCTCAGGAGGCTCCACCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR204743 representing NM_053764
Red=Cloning site Green=Tags(s) MPGKPKHLGVPNGRMVLAVSDGELTSTSGSQAQGEGRGSSLSIHSLPSGPSSPFSTDEQPVASWAQSFER LLQDPRGLAYFTEFLKKEFSAENVTFWQACERFQQIPASDTKQLAQEAHNIYHEFLSSQALSPVNIDRQA WLSEEVLAQPRPDMFRAQQLQIFNLMKFDSYARFVKSPLYQECLLAEAEGRPLREPGSSHLGSPDTARKK PKLKPGKSLPLGVEELGQLPLAEGRPLRKSFRREMPGGAVNSALRRESQGSLNSSASLDLGFLAFVSSKS ESHRKSLGSGEGESESRPGKYCCVYLPDGTASLALARPGLTIRDMLAGICEKRGLSLPDIKVYLVGKEQK ALVLDQDCTVLADQEVRLENRITFQLELVGLERVVRISAKPTKRLQEALQPILAKHGLSLDQVVLHRPGE KQLVDLENLVSSVASQTLVLDTLPDAKTREASSIPPCRSQGCLPRTQTKDSHLPPLSSSLSVEDASGSTG KRQTCDIEGLVELLNRVQSSGAHDQRGLLRKEDLVLPEFLQLPSQRPGSQEAPP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_053764 |
ORF Size | 1632 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_053764.1, NP_446216.1 |
RefSeq Size | 2854 bp |
RefSeq ORF | 1635 bp |
Locus ID | 114705 |
UniProt ID | O08773 |
MW | 59.5 kDa |
Gene Summary | binds Galpha(i1)GDP and acts as a GTPase activator by maintaining G-protein subunit dissociation; regulates G-protein mediated signaling [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN204743 | Rgs14 (untagged ORF) - Rat regulator of G-protein signaling 14 (Rgs14), (10 ug) |
CNY 6,088.00 |
|
RR204743L3 | Lenti ORF clone of Rgs14 (Myc-DDK-tagged ORF) - Rat regulator of G-protein signaling 14 (Rgs14), (10 ug) |
CNY 8,930.00 |
|
RR204743L4 | Lenti ORF clone of Rgs14 (mGFP-tagged ORF) - Rat regulator of G-protein signaling 14 (Rgs14), (10 ug) |
CNY 9,790.00 |