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Itch (NM_001005887) Rat Tagged ORF Clone
CAT＃: RR204734
- TrueORF®
Itch (Myc-DDK-tagged ORF) - Rat itchy E3 ubiquitin protein ligase homolog (mouse) (Itch), (10 ug)
"NM_001005887" in other vectors (3)
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CNY 10,930.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR204734 representing NM_001005887
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTAGTCTGACCATGAAATCTCAACTTCAGATTACTGTCATCTCAGCCAAACTTAAAGAAAATAAAA AGAATTGGTTTGGACCAAGTCCTTATGTAGAAGTCACAGTAGATGGGCAGTCAAAGAAGACAGAAAAATG CAACAATACAAACAGCCCCAAGTGGAAGCAGCCCCTCACAGTTATCGTTACCCCTATGAGTAAATTATGT TTTCGTGTGTGGAGTCACCAGACCCTGAAATCTGATGTTTTATTGGGAACTGCTGGATTAGATATTTATG AAACATTAAAGTCAAACAATATGAAACTTGAGGAAGTAGTTATGACTTTGCAGCTTGTAGGTGACAAAGA GCCAACAGAGACAATGGGAGATTTGTCAGTTTGTCTTGATGGGCTGCAAGTAGAAGCTGAAGTTGTTACT AATGGTGAAACTTCATGCTCAGAGGGTGCTACACAGAATGACGCTGGCTGCAGACCCAAAGATGAAACAA GAGTGAGCACAAATGGATCAGAGGACCCTGAGGTTGCAGCCTCGGGGGAGAACAAGAGGGCCAATGGGAA CAATTCTCCATCCCTTTCAAATGGTGGTTTTAAGCCTTCTAGACCTCCTAGACCTTCAAGACCACCTCCA CCCACCCCACGAAGACCAGCTTCTGTCAATGGCTCACCATCCACGAACTCTGACAGCGATGGATCTAGCA CAGGCTCTTTGCCACCCACAAATACAAATGTAAATTCAAGTGCATCGGAAGGAGCAGCATCTGGGTTAAT AATTCCTCGTACCATCTCTGGGGGCTCAGGCCCTAGGCCGCTGAATACTGTCAGTCAAGCTCCCCTCCCA CCTGGGTGGGAGCAGAGAGTAGATCAGCATGGGCGTGCTTACTATGTAGACCATGTTGAAAAGCGAACAA CACGGGATAGACCGGAACCTCTACCTCCTGGCTGGGAACGGCGTGTGGACAATATGGGACGTATTTATTA TGTTGACCATTTCACGAGAACCACAACATGGCAGAGGCCGACCTTGGAATCTGTCCGGAACTATGAGCAG TGGCAGCTCCAGCGTAGTCAACTTCAGGGAGCAATGCAGCAGTTTAACCAGAGATTCATCTTTGGGAATC AAGATTTGTTTGCTACATCACAAAACAAAGAGTTTGATCCCCTTGGTCCTTTGCCCCCTGGATGGGAGAA GAGAACTGATAGCAACGGCAGAGTGTATTTTGTCAACCACAACACTCGGATTACACAGTGGGAAGACCCC AGAAGCCAAGGTCAGTTAAATGAAAAGCCCTTACCAGAAGGCTGGGAAATGCGATTCACAGTGGATGGAA TTCCATATTTTGTGGACCACAATAGAAGAGCAACTACCTACATAGATCCACGCACAGGAAAGTCAGCCTT AGACAACGGACCCCAGATAGCCTATGTTCGGGACTTCAAGGCAAAAGTTCAGTATTTCCGGTTCTGGTGT CAGCAACTGGCCATGCCACAGCATATAAAGATCACAGTGACAAGAAAAACATTGTTTGAGGATTCCTTTC AACAGATCATGAGCTTCAGTCCGCAAGATCTGAGAAGACGTTTGTGGGTGATTTTCCCAGGAGAAGAAGG TTTAGATTATGGAGGTGTAGCAAGAGAATGGTTCTTTCTTTTGTCACATGAAGTGTTGAACCCAATGTAT TGCCTGTTTGAATATGCAGGGAAGGATAACTACTGCCTGCAGATAAACCCCGCTTCTTATATCAACCCAG ACCACCTGAAATACTTCCGTTTTATTGGCAGATTTATTGCCATGGCTCTATTCCATGGGAAATTCATAGA TACCGGATTTTCTTTACCATTCTATAAACGTATCTTGAATAAACCAGTGGGACTTAAGGATTTAGAATCT ATCGATCCAGAATTTTACAATTCTCTCATCTGGGTTAAAGAAAACAATATCGAGGAATGTGGTTTGGAAA TGTACTTCTCAGTTGATAAAGAAATTCTGGGTGAAATTAAGAGTCATGATTTGAAACCCAATGGTGGCAA TATTCTGGTGACAGAAGAAAATAAGGAAGAGTACATCAGGATGGTCGCTGAGTGGAGGTTGTCTCGGGGA GTTGAAGAGCAGACACAAGCTTTCTTTGAGGGCTTTAATGAAATTCTTCCTCAGCAATATTTGCAATATT TTGATGCGAAGGAATTAGAGGTCCTTTTGTGTGGGATGCAGGAGATCGATTTGAATGACTGGCAGAGACA CGCCATCTACCGTCACTATACCAGGACGAGCAAGCAGATCGTGTGGTTTTGGCAGTTTGTTAAAGAAATT GATAACGAGAAGAGGATGCGACTTCTGCAGTTTGTTACCGGAACCTGCCGATTGCCAGTGGGAGGATTTG CTGACCTCATGGGGAGTAATGGACCACAGAAGTTCTGCATCGAAAAAGTTGGCAAAGAAAATTGGTTACC CAGAAGCCATACTTGTTTTAACCGCCTGGACCTCCCACCCTACAAGAGCTATGAGCAACTGAAGGAAAAG CTGTTATTTGCCATTGAAGAAACTGAAGGATTTGGACAAGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR204734 representing NM_001005887
Red=Cloning site Green=Tags(s) MGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPMSKLC FRVWSHQTLKSDVLLGTAGLDIYETLKSNNMKLEEVVMTLQLVGDKEPTETMGDLSVCLDGLQVEAEVVT NGETSCSEGATQNDAGCRPKDETRVSTNGSEDPEVAASGENKRANGNNSPSLSNGGFKPSRPPRPSRPPP PTPRRPASVNGSPSTNSDSDGSSTGSLPPTNTNVNSSASEGAASGLIIPRTISGGSGPRPLNTVSQAPLP PGWEQRVDQHGRAYYVDHVEKRTTRDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQ WQLQRSQLQGAMQQFNQRFIFGNQDLFATSQNKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDP RSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRATTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWC QQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMY CLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLES IDPEFYNSLIWVKENNIEECGLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRG VEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYTRTSKQIVWFWQFVKEI DNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEK LLFAIEETEGFGQE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001005887 |
| ORF Size | 2562 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001005887.1, NP_001005887.1 |
| RefSeq Size | 2565 bp |
| RefSeq ORF | 2565 bp |
| Locus ID | 311567 |
| MW | 97.7 kDa |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN204734 | Itch (untagged ORF) - Rat itchy E3 ubiquitin protein ligase homolog (mouse) (Itch), (10 ug) |
CNY 10,930.00 |
|
| RR204734L3 | Lenti ORF clone of Itch (Myc-DDK-tagged ORF) - Rat itchy E3 ubiquitin protein ligase homolog (mouse) (Itch), (10 ug) |
CNY 12,730.00 |
|
| RR204734L4 | Lenti ORF clone of Itch (mGFP-tagged ORF) - Rat itchy E3 ubiquitin protein ligase homolog (mouse) (Itch), (10 ug) |
CNY 13,970.00 |
