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Manba (NM_001031655) Rat Tagged ORF Clone
CAT＃: RR204699
- TrueORF®
Manba (Myc-DDK-tagged ORF) - Rat mannosidase, beta A, lysosomal (Manba), (10 ug)
"NM_001031655" in other vectors (3)
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CNY 11,310.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MGC116230; MGC125196 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR204699 representing NM_001031655
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCATCTCCACCTGCTCTTTCTGTTGGCGCTGTGCGGGGCAGGCTGTATGGCCGCAGGTCCCAGCTACA GCCTGAGAGGGAGCTGGCGGGTGAGCAACGGGAATAGTTCCCTGGAGCTGCCGGCTACTGTCCCTGGCTA TGTGCACAGCGCCCTGCAGCAGCACGGGCTGATCCAGGATCCTTACTACAGATTTAATGACCTGAACTAC AGATGGATTTCCTTAGATAACTGGACCTACAGCACAGAATTTAAAATCCCCTTTAATCGCAGTGAATGGC AGAAGGTAAAGTTGATTTTTGACGGAGTCGATACAGTTGCAGAAATCCTGTTCAATAATGTCACTATTGG GAAGACAGACAACATGTTCACCAGATATAGCTTTGACGTCACCAATGTGGTCAAAGACGTGAACTCCCTA AAGCTGCGGTTCCAGTCAGCAGTGCAGTATGCTGAGTGTCAGAGCAAAGCTCACACTCAATACCGGGTGC CCCCAGAGTGCCCCCCAGTGGAACAGAAGGGTGAATGCCACGTCAACTTCATCCGAAAGGAGCAGTGCTC CTTCAGCTGGGACTGGGGGCCTTCCTTTCCCTCTCAAGGAATCTGGAAAGACGTTAGAATCGAAGCCTAT AACATCGCTCATCTGGATCACCTGACATTTTTGCCATTGTATGATAACACTTCTCAGGCGTGGACTATTG AAATAGAGGCTTCTTTTGATGTTGTCAGCACAAAGCCAGTTGGTGGTCAGGTGACTATAGCCATCCCCGA ACTGAAAACACAGCAGGCAAACCACATTGAACTTCAACATGGGCAAAGGATTGTTAAACTCCTTGTCAAA ATTAGGAAGGACGTCACCGTGGAGACCTGGTGGCCTCATGGACATGGGAACCAGACTGGGTACAACACGA CAATTCTCTTTGCGCTGGATGGAGGCTTAAAAATTGAAAAAGCAGCCAAGGTTTATTTCAGAACAGTGCA ACTTATAGAAGAACCTATAACAGGGTCTCCTGGTCTGAGTTTCTACTTCAAAATTAATGGGCTTCCTATA TTTCTGAAAGGCTCAAACTGGATCCCAGCTGACTCATTCCAAGACAAAGTCACTTCTGAACTGCTGCAGC TCCTTCTCCAGTCCGCCGTGGATGCTAACATGAACACTCTGCGCGTGTGGGGGGGTGGCATCTACGAGCA GGATGAATTCTATGCACTGTGTGACGAACTTGGAATAATGGTGTGGCAGGACTTCATGTTTGCCAGTGCC CTCTATCCAACTGAGCCAGGCTTCCTAGAGTCAGTGAGAAAAGAAGTCACTTACCAGGTCAGGAGACTGA AGTCTCACCCCTCCGTCATCATCTGGAGTGGTAATAATGAGAATGAAGTGGCTCTCAGGGTGAACTGGTT CCATGTAAACCCCAGGGACCTGGGGACTTACATCAACGACTATGTGACCCTCTACGTGAAAACCATCAGG GAGATTGTCTTATCGGAAGATAGGAGTCGTCCGTTCATTGCATCCAGCCCAACCAATGGGGTCAAAACCA TGACGGAAGGCTGGATCTCTAAGGACCCTTATAGCACCCAGTATGGCGACATGCATTTTTACGACTATTT CAGTGACTGCTGGGACTGGAAGGTCTTTCCAAAAGCTCGATTAGTGTCTGAATATGGCTACCAGTCCTGG CCTTCCTTCAGCACACTACAAAAGGTCTCTCGTCAAGAGGACTGGTCTTACAGCAGCCGCTTCTCCCTTC ATCGGCAGCATCATGGAAATGGCAACAATGAAATGCTTCACCAGGTACAGCTTCATTTCCAACTCCCCCA AAGGAGAGACCCAGTGCGTGCATTTAAAGATACCATCTACCTCACTCAGGTGATGCAGGCCCAGTGTATC AAAACAGAAACCGAGTTCTACCTGCGCAGCCGCAGCGAGATAGTGAATGGAGAAGGCCACACCATGGGGG CACTGTACTGGCAGCTGAATGATATCTGGCAGGCTCCTTCCTGGGCCTCTCTTGAGTACGGAGGGAAATG GAAAATGCTTCATTACTTCGCTCAGCGTTTCTTCGCTCCGCTGCTGCCAGTCGGTTTTGAAGATGAAGGC GTTTTTTATGTCTACGGTGTGTCAGATCTTCACAAGGACTACCCCACAAAGCTCACCGTGAGGCTCCACC GCTGGAGCTCCCAGAAGCCTTTGTGCACCTTCGTGAGTTTGAGTGCTGTGATTAAGGCAGGGGAGGCGAT GGTTCTGTTCCAGATGCCAGTGTCTAAGCTGCTGAAGAGGTGTAAAGAATGCACAAGGGACACCTGTGTG GTTTCCTTTTACCTCTCAACTGACAATGAACTCTTCAGTCCAACCAACTACCATTTCCTGTCCTCCCTGA AGGATGCCAAAGGGATGGTCAAGGCCAACATCACTGTCAGCATCTCTCAGAAGGGTGATCTATTTGTTTT TGATCTGAAATCGTCAACATCAGCCATCACTCCCTTTGTTTGGTTGGATGTAGGAAGCATCCCGGGGAGA TTTAGTGACAACGGCTTCCTCATGATTAAGAAGGAGCTCTCGGTACTGTTTTACCCATGGAAGCCCACCA GCAAGAGTGAGCTGCAGCAGGCCTTCAGTGTCACCTCCCTGACAGATCTGTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR204699 representing NM_001031655
Red=Cloning site Green=Tags(s) MHLHLLFLLALCGAGCMAAGPSYSLRGSWRVSNGNSSLELPATVPGYVHSALQQHGLIQDPYYRFNDLNY RWISLDNWTYSTEFKIPFNRSEWQKVKLIFDGVDTVAEILFNNVTIGKTDNMFTRYSFDVTNVVKDVNSL KLRFQSAVQYAECQSKAHTQYRVPPECPPVEQKGECHVNFIRKEQCSFSWDWGPSFPSQGIWKDVRIEAY NIAHLDHLTFLPLYDNTSQAWTIEIEASFDVVSTKPVGGQVTIAIPELKTQQANHIELQHGQRIVKLLVK IRKDVTVETWWPHGHGNQTGYNTTILFALDGGLKIEKAAKVYFRTVQLIEEPITGSPGLSFYFKINGLPI FLKGSNWIPADSFQDKVTSELLQLLLQSAVDANMNTLRVWGGGIYEQDEFYALCDELGIMVWQDFMFASA LYPTEPGFLESVRKEVTYQVRRLKSHPSVIIWSGNNENEVALRVNWFHVNPRDLGTYINDYVTLYVKTIR EIVLSEDRSRPFIASSPTNGVKTMTEGWISKDPYSTQYGDMHFYDYFSDCWDWKVFPKARLVSEYGYQSW PSFSTLQKVSRQEDWSYSSRFSLHRQHHGNGNNEMLHQVQLHFQLPQRRDPVRAFKDTIYLTQVMQAQCI KTETEFYLRSRSEIVNGEGHTMGALYWQLNDIWQAPSWASLEYGGKWKMLHYFAQRFFAPLLPVGFEDEG VFYVYGVSDLHKDYPTKLTVRLHRWSSQKPLCTFVSLSAVIKAGEAMVLFQMPVSKLLKRCKECTRDTCV VSFYLSTDNELFSPTNYHFLSSLKDAKGMVKANITVSISQKGDLFVFDLKSSTSAITPFVWLDVGSIPGR FSDNGFLMIKKELSVLFYPWKPTSKSELQQAFSVTSLTDLY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001031655 |
| ORF Size | 2643 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001031655.1, NP_001026825.1 |
| RefSeq Size | 3688 bp |
| RefSeq ORF | 2646 bp |
| Locus ID | 310864 |
| UniProt ID | Q4FZV0 |
| MW | 101.2 kDa |
| Gene Summary | Exoglycosidase that cleaves the single beta-linked mannose residue from the non-reducing end of all N-linked glycoprotein oligosaccharides.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN204699 | Manba (untagged ORF) - Rat mannosidase, beta A, lysosomal (Manba), (10 ug) |
CNY 11,310.00 |
|
| RR204699L3 | Lenti ORF clone of Manba (Myc-DDK-tagged ORF) - Rat mannosidase, beta A, lysosomal (Manba), (10 ug) |
CNY 13,020.00 |
|
| RR204699L4 | Lenti ORF clone of Manba (mGFP-tagged ORF) - Rat mannosidase, beta A, lysosomal (Manba), (10 ug) |
CNY 14,250.00 |
