Ssc5d (NM_001134545) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1565772
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR204574 representing NM_001134545 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGGTCTTGGCGTGTCTTCTTGCAATGCTGGTGGGGATCCAGGCTATAGAGCGACTCCGCCTGGCTG ATGGCCCTCATGGCTGTGCAGGACGCCTGGAGGTCTGGTACAGCGGGCGCTGGGGCACCGTGTGTGATGA CGGATGGGACCTTCGGGATGCTGCAGTGGCCTGCCGTGTGTTGGGCTGCGGAGGGGCGCTAGCCGCCCCC GGGGGTGCCTTCTTTGGGGAGGGCACTGGGCCCGTGTGGCTCAGTGAGCTGGCCTGTCGGGGCAGTGAGG GACAGCTGGGTATCTGTCCCCACCGGGGCTGGAAGGCTCATATCTGCTCTCACGAGGAGGACGCGGGCGT CGTCTGTGTAGGTCAGCGTGCAGCTAACTCTAGAGAAGACTCAGTGTCCCTGCTGGATGGGGACCCATGG CTGGCACTGTCTGGGGAGCTGAGCCCCAGCTCAGAGGAGCCCCCTATAACTCATGCTCCCCAGCCGGCAG CAAGCTCCCAGAATGGCCCCCGAAAGAAGAACCCCCGGCCACCCAAGCAGGTCAAGTCCACCCGAGCCCC TGTACTGACAAATGGAGCTCCCCGCCAAGAGCGGCTGCGTCTGGTCTCAGGTCCCCATGGATGTGCCGGC CGCCTGGAAGTCTGGCACGGTGGACGTTGGGGTACTGTCTGCGATGATGGATGGGACCTCCGAGACGCAG CAGTGGCCTGTAGGGAGCTAGGATGTGGGGGAGCGCTGGCCGCCCCTGGAGGTGCCAGATTCGGACCTGG TGAGGGACCCGTGTGGATGGACGATGTCGGGTGTGGAGGAGGAGAGGAGGCCCTCCGGGACTGTCCCCGA AGCCCCTGGGGCCGGAGCAACTGTGACCACACAGAGGATGCAGGGCTGGTCTGTACTGGTCCTGCACCCA GGTTGCGCCTTGCTGATGGTCCCCACGGCTGTGCTGGGCGCCTGGAGGTTTGGCATGGTGGCCGCTGGGG GTCGGTATGTGACGATGCTTGGGATCTTCGTGATGCCGCTGTGGCCTGCAGGGAGCTGGGCTGTGGGGGT GCCCTGGCTGCCCCAGGGGGTGCCTTCTTTGGTGAGGGGGCTGGACCCATCATCCTGGATGACCTCAGAT GTCGAGGAAACGAGACAGCCTTACGATTCTGCCCTGCGAGGCCCTGGGGTCAGCATGACTGTCACCATCG GGAGGATGCTGGGGCTGTGTGTGATGGCATGCCTCTCGGTGGCGATGTGCAGCCTACAGCTCCTGCAGTG GACAGCAACAGAACAACACCCAGGCTCCAGTCTACCTCAGTGGGTCATACGCCAGGCCCAGCAGGCAGTT GGCCTCCTCCTGCTTCTCCTACTGCCCCTCCAGAGCCTGGACCTGAAGCTGGGTCCCCCCAGTTGCGCCT GGTGGCTGGACCCAGCAGGTGCTCTGGTCGGCTGGAGGTATGGCATGATGGACGCTGGGGGACAGTATGT GATGACAGCTGGGACATGAGAGACTCAGCTGTGGTCTGCCGGGAGCTGGGCTGTGGGGGGCCCCGACAGC CAGACCCTGCAGCAGGCCGCTTTGGCTGGGGCGCAGGCCCCATCTGGCTGGATGATGTAGGTTGTGTGGG GACGGAGGCTTCACTGTCAGAATGCCCTGCTGCTTCCTGGGGGAAACACAATTGCGCCCACAATGAGGAT GTCGGGGTTACCTGCACCGGGACCCCCGGCCTGGACACCATCTCAGACCCTTTCAGTTGGAGCTGGCTCC CTGGGCTGGGTAGAGATCAGGATGCCTGGCTCCCAGGAGAGCTGGCCACCAAGCCTTCTGCCAGTCTCAC CTCCAGTGTGCTACAGAAAACCACAAAGGCTCCAGGGAAAGTGCCCAAGAGTACCAAGAAGTGGGTGACT AAAAATGCAAAGAGACCAACCACTCAGCCCCCTGGGATACCAATCACTAAACGCCCCAGGGTCCCAGGCA CCCCAACCTCCCTAAGCCCAACCCCACGTACCTCTGAGTTACCAAAGAGACTTACTGCCGAGGCTCCTCG CAGACAGACCTCACACACCACCGAAAGGCTGACCCCTAGGGTCCCCTGGGAGTGGACCTCAGAGACCGCG GCATCACTGTCCACTCAAGGCCCCCAAGAAGTGACCTCTGAGGCCACCACCGCACAAACCCCTCAGACCT CCTTGGAGCCATTTGGTGAGAACCCAGAAGGCTCTCTAGAATCTTCCCAGGATCCAGCTGCCACCCCGAC TGCTGGAGTTCCCGTGCCATCGGGTCCCTTCCGGGTTCGTCTGGCTGATGGGCCCAACAGGTGTGCTGGC CGGCTAGAGGTGTGGCATGCCGGGCTCTGGGGGACAGTCTGTGATGACAGCTGGGACCTCCGGGATGCCA CAGTGGCCTGCTGGGAACTGGGTTGCGGAAAGGTCCGGCCCCGAGTAGGCAAAACCCACTATGGCCCTGG CACTGGGCCCATCTGGTTGGATGACATGGGCTGTAAGGGAAGTGAGACGTCACTGAGTGACTGCCCCTCG GGGACATGGGGGAAGCACAACTGTGACCACGAAGAAGACGTGGTGCTCACCTGCACTGGCTACACAGGGG ATGACGATTACCCTTCCTGGACCTGGGACCCCACTTCGGAAGAGGACCTGACCAAAAGGACCACAGTGGC CACACAGCCTGGACATACACCTTCCTGGGCTACTACCACAAACCCTGAAGTTCTCTCTCCAGCAACGCAG AGCCTTCCAGACATAGATGACCAGGGTAGTTATGAGCTCTCCTGGACATGGGATACACCTTCAGGAAGGA GTCTGTCCAAAGGGACCCCCACCACAACCAAACCTGGATCCACAGTTACCACTAGCACCAGAAAGAGTCC AGGCCATCCCTTTCCAGCTCCAAGGGCCCGTGCAAACACAGGTTTACCAAGGAAGCCAAAACCTGAGCGC CGGACGCTGCAGCCCACCTCAGCCAGGACGACACACACCACCTCATCCCCGGCTTCCTACACCTCACCAG AGCCTTCTGGCTCACAGCAGACCTCTGGCTCCTGGCCACAACTCTTCCCAGACTTGGCTTCAAAGCAGGA GGGGACGTCTACCCCTCCCAAAACATCTCCACTCACCCCAGGACTTCCCTCCCCAACAAACTTTGCCTTG TCCACTACTGATGCCAGTTTGCTTCCAACACGTCCCCCAGAGCTTTCAGGCAGCCCAACATCCAACTCCC CTGAAGGGCTAACCTCTGACTCCCCCATGCTATTAGAAGTGAGCAGCCTTTCCCCTACCTCAGAGCTGAC GCCAGGATCTGACATGACACCAGACCTGGGCACAGCACCCAAAATAATCCCAGAAAGTTCTGAGTCCTCA GACCTCCCCACGAGCCCCAGCACCCACACACAACCATTCACAGCCTCTTACCCTACCAGCATCCCTCACC CCACCACCATCCCTCATCCCACCACTACCCCTCATCCTAGAGCCCCCTCCCACCCCACCACCATCCCTCA ACTCAACACAACTTCCTACCCCACCATGGCCCTGCAACCTACCACCAACCCTCAGCATCCCGGGAGCCCT CATCCCACCACAAGTCCTCAACCTCCCACCAACACTCACTCTTCTTCAACCTCTGCCACCCCCACAAAGT CCCTTCCATCCTCCCCAGAGACAGAATTTTCCTCTCCTACTCCACAACCAAGGGTCAAGCCTAGCCTACA CCCAGATCTGACCTTTGTAGGAGTCCCGCCCACAGGCAAATCCCAGATGCAGAACCTAGAGTTCTCTCTA GCCTCAGAGTCTGGCCCCTCCAGGCCCTCTCCAGCCCCAAGCATGGACCCACTATCCACAGAGGTCTTCA AACCACCCAGAAGCCAGACCTTACACTCAGTTTCAGAACCCATGGTGACCGCTGACCTTGCCATCTCCTC TATGGCCCACCCATCTGACCAACCTCCCCCAGATCGCCTCGCTCAAGGGCCAACTTCTAATCACAACCCA GGTCCCTTTGGGCCATGTGTCTCCTCAGTACCACCTGTAAGGGTCATGGCTTGTGAGCCACCTGCCTTGG TGGAGCTGGTGGCTGCTGTGAGGGAGGTGGGTGACCAGCTGCAGAGGCTGACCCGGGTCCTGGAGCAGGA CCGGCAGGAGCGCCAAGTCCTGGGGCTGGGCCTAGTCAAGTTGGTAGAGGCTGCCCAGGGGTTGGGGCAG CTAAGTGAGACTGTAAAGAGGCTTGCAGAGGTGGCCTGGCCCCCCAGCACACCTGTGCCCACAACCACCA CCACAGAGGAGGAGGAGAGGCCTCTGAGAGGAGATGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR204574 representing NM_001134545 Red=Cloning site Green=Tags(s) MRVLACLLAMLVGIQAIERLRLADGPHGCAGRLEVWYSGRWGTVCDDGWDLRDAAVACRVLGCGGALAAP GGAFFGEGTGPVWLSELACRGSEGQLGICPHRGWKAHICSHEEDAGVVCVGQRAANSREDSVSLLDGDPW LALSGELSPSSEEPPITHAPQPAASSQNGPRKKNPRPPKQVKSTRAPVLTNGAPRQERLRLVSGPHGCAG RLEVWHGGRWGTVCDDGWDLRDAAVACRELGCGGALAAPGGARFGPGEGPVWMDDVGCGGGEEALRDCPR SPWGRSNCDHTEDAGLVCTGPAPRLRLADGPHGCAGRLEVWHGGRWGSVCDDAWDLRDAAVACRELGCGG ALAAPGGAFFGEGAGPIILDDLRCRGNETALRFCPARPWGQHDCHHREDAGAVCDGMPLGGDVQPTAPAV DSNRTTPRLQSTSVGHTPGPAGSWPPPASPTAPPEPGPEAGSPQLRLVAGPSRCSGRLEVWHDGRWGTVC DDSWDMRDSAVVCRELGCGGPRQPDPAAGRFGWGAGPIWLDDVGCVGTEASLSECPAASWGKHNCAHNED VGVTCTGTPGLDTISDPFSWSWLPGLGRDQDAWLPGELATKPSASLTSSVLQKTTKAPGKVPKSTKKWVT KNAKRPTTQPPGIPITKRPRVPGTPTSLSPTPRTSELPKRLTAEAPRRQTSHTTERLTPRVPWEWTSETA ASLSTQGPQEVTSEATTAQTPQTSLEPFGENPEGSLESSQDPAATPTAGVPVPSGPFRVRLADGPNRCAG RLEVWHAGLWGTVCDDSWDLRDATVACWELGCGKVRPRVGKTHYGPGTGPIWLDDMGCKGSETSLSDCPS GTWGKHNCDHEEDVVLTCTGYTGDDDYPSWTWDPTSEEDLTKRTTVATQPGHTPSWATTTNPEVLSPATQ SLPDIDDQGSYELSWTWDTPSGRSLSKGTPTTTKPGSTVTTSTRKSPGHPFPAPRARANTGLPRKPKPER RTLQPTSARTTHTTSSPASYTSPEPSGSQQTSGSWPQLFPDLASKQEGTSTPPKTSPLTPGLPSPTNFAL STTDASLLPTRPPELSGSPTSNSPEGLTSDSPMLLEVSSLSPTSELTPGSDMTPDLGTAPKIIPESSESS DLPTSPSTHTQPFTASYPTSIPHPTTIPHPTTTPHPRAPSHPTTIPQLNTTSYPTMALQPTTNPQHPGSP HPTTSPQPPTNTHSSSTSATPTKSLPSSPETEFSSPTPQPRVKPSLHPDLTFVGVPPTGKSQMQNLEFSL ASESGPSRPSPAPSMDPLSTEVFKPPRSQTLHSVSEPMVTADLAISSMAHPSDQPPPDRLAQGPTSNHNP GPFGPCVSSVPPVRVMACEPPALVELVAAVREVGDQLQRLTRVLEQDRQERQVLGLGLVKLVEAAQGLGQ LSETVKRLAEVAWPPSTPVPTTTTTEEEERPLRGDV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001134545
ORF Size	4308 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001134545.1, NP_001128017.1
RefSeq Size	4495 bp
RefSeq ORF	4311 bp
Locus ID	308341
MW	151.6 kDa

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