Hsd3b7 (NM_139329) Rat Tagged ORF Clone
CAT#: RR204318
- TrueORF®
Hsd3b7 (Myc-DDK-tagged ORF) - Rat hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (Hsd3b7), (10 ug)
"NM_139329" in other vectors (3)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Cca2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR204318 representing NM_139329
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGACTCTGCACAGGTGCCAGCACTGGTATACCTGGTCACAGGTGGCTGTGGCTTCCTGGGGGAGC ATATTGTTCGAATGCTGCTGGAGTGGGAGCCCAGGCTCCGGGAGCTGCGTGTCTTTGACCTGCACCTGAG TTCTTGGCTGGAGGAGCTGAAAACAGGGCCTGTGCAGGTGACTGCCATCCAGGGGGATGTGACTCAGGCC CACGAGGTGGCAGCAGCCATGGCTGGATCTCATGTGGTCATCCATACAGCTGGGCTGGTAGATGTGTTTG GGAAGGCCAGTCCAGAGACCATCCACAAAGTCAATGTGCAGGGCACACAGAATGTGATTGACGCTTGTGT GCAGACCGGGACTCGGCTCCTGGTCTACACGAGTAGCATGGAAGTGGTAGGGCCTAATGTCAAGGGCCAC CCCTTCTACAGGGGCAATGAAGACACCCCATATGAGGCCATCCACAGGCATCCCTACCCATGTAGCAAGG CCCTCGCTGAGCAGTTGGTCCTCGAGGCCAATGGAAGGAAGGGACTGCGCTTTGGAGGTCGGCTGTTTCG GGCCATCCCAGCTTCCGTGGAGCATGGTCGGGTCTACGTTGGCAATGTGGCTTGGATGCATATACTGGTG GCCCGAGAGCTGGAGCAGCGGGCAGCACTCATGGGTGGCCAGGTGTATTTCTGCTATGATAAGTCACCTT ATAAAAGCTACGAGGACTTCAACATGGAGTTTCTGAGTCCCTGTGGACTTCGGCTGATAGGCACCCACCC ACTGCTGCCCTACTGGCTACTGGTGCTGCTGACTGCCCTCAACGCTCTGCTGCAGTGGCTGCTTCGGCCG CTGGTGCTGTACACACCCCTGCTGAATCCCTACACTCTGGCTGTGGCCAACACCACCTTTACTGTCAGCA CCAACAAGGCACAGCGCCATTTCGGCTACAAGCCCCTCTTCTCATGGGAAGAGAGCAGGGCCCGCACCAT TCACTGGGTGCAGGCCATGGAGGGTTCCGCCTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR204318 representing NM_139329
Red=Cloning site Green=Tags(s) MADSAQVPALVYLVTGGCGFLGEHIVRMLLEWEPRLRELRVFDLHLSSWLEELKTGPVQVTAIQGDVTQA HEVAAAMAGSHVVIHTAGLVDVFGKASPETIHKVNVQGTQNVIDACVQTGTRLLVYTSSMEVVGPNVKGH PFYRGNEDTPYEAIHRHPYPCSKALAEQLVLEANGRKGLRFGGRLFRAIPASVEHGRVYVGNVAWMHILV ARELEQRAALMGGQVYFCYDKSPYKSYEDFNMEFLSPCGLRLIGTHPLLPYWLLVLLTALNALLQWLLRP LVLYTPLLNPYTLAVANTTFTVSTNKAQRHFGYKPLFSWEESRARTIHWVQAMEGSAW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_139329 |
ORF Size | 1014 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_139329.1, NP_647545.1 |
RefSeq Size | 1795 bp |
RefSeq ORF | 1017 bp |
Locus ID | 246211 |
UniProt ID | O35048 |
MW | 37.8 kDa |
Gene Summary | protein that is expressed in under growth-arrest conditions [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN204318 | Hsd3b7 (untagged ORF) - Rat hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (Hsd3b7), (10 ug) |
CNY 3,990.00 |
|
RR204318L3 | Lenti ORF clone of Hsd3b7 (Myc-DDK-tagged ORF) - Rat hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (Hsd3b7), (10 ug) |
CNY 6,080.00 |
|
RR204318L4 | Lenti ORF clone of Hsd3b7 (mGFP-tagged ORF) - Rat hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (Hsd3b7), (10 ug) |
CNY 6,650.00 |