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Cpeb1 (NM_001106276) Rat Tagged ORF Clone
CAT＃: RR204288
- TrueORF®
Cpeb1 (Myc-DDK-tagged ORF) - Rat cytoplasmic polyadenylation element binding protein 1 (Cpeb1), (10 ug)
"NM_001106276" in other vectors (3)
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CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR204288 representing NM_001106276
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTTTTCTCTGGAAGAAGAATCAGGAAGGATAAAGGACTGCTGGGACAACCAAGAGGTTCCTGCAC TCTCTACATGTAGCAATGCCAATATCTTTCGAAGGATAAATGCCATTTTGGATGATTCACTGGACTTCAG TAAAGTCTGCACAACACCTATAAATCGAGGAATTCATGATCAGTTGCCAGACTTCCAGGACTCTGAAGAA GCAATTACAAGCAGGATGCTTTTCCCCACCTCTGCGCAAGAATCTCCCCGCGGCCTCCCAGATGCAAACG GTTTGTGCCTTGGCCTGCAGTCACTCAGTCTGACTGGCTGGGACCGACCCTGGAGCACCCAGGACTCAGA CTCCTCAGCCCAGAGCAACACACAGTCAGTATTAAGCATGCTCCAAAATCCACTGGGAAATGTCTTAGGA AAAACCCCCTTGAGCTTCCTGTCTCTGGATCCCCTTGGGTCTGACTTGGACAAGTTCCCAGCGCCCTCAG TTAGAGGATCTCGCCTGGACACCCGGCCTATCCTGGACTCCCGTTCTAGCAGCCCCTCTGACTCAGACAC GAGTGGCTTCAGCTCTGGATCAGATCATCTCTCAGATTTGATTTCAAGCCTTCGCATTTCCCCTCCTCTG CCCTTTCTTTCTATGACGGGAAATGGTCCCAGAGACCCTCTAAAGATGGGAGTTGGGTCTCGAATGGACC AAGAGCAAGCTGCTCTTGCTGCAGTTGCTCCCTCTCCAACCAGTGCTCCAAAGAGATGGCCAGGAACTTC TGTGTGGCCATCTTGGGACCTTCTCGGAGCTCCTAAAGACCCATTCAGCATAGAGAGAGAGGCTAGACTC CACAGGCAGGCCGCAGCTGTGAATGAAGCTACATGTACCTGGAGTGGCCAGCTTCCTCCCCGGAACTACA AGAACCCCATCTACTCATGCAAGGTGTTCCTAGGAGGTGTTCCTTGGGACATTACAGAAGCTGGATTGGT TAACACCTTCCGTGTTTTTGGCTCTCTGAGTGTGGAGTGGCCTGGTAAGGATGGCAAGCACCCCCGGTGT CCTCCCAAAGGTAATATGCCTAAAGGGTATGTGTATTTGGTGTTTGAACTAGAGAAGTCCGTCCGGGCCT TGCTTCAGGCTTGTTCTCATGACCCACTGAGCCCAGATGGCCTAAGTGAATATTATTTCAAGATGTCCAG CCGAAGGATGCGCTGCAAAGAAGTACAGGTAATCCCTTGGGTCTTGGCTGACAGCAACTTTGTCTGGAGC CCATCTCAGAGGCTGGATCCCAGCAGGACGGTGTTTGTTGGTGCCTTGCACGGGATGCTCAATGCTGAGG CTCTGGCTGCCATCTTGAATGACCTATTTGGTGGAGTGGTGTATGCTGGGATTGACACAGATAAGCACAA GTACCCCATTGGGTCTGGTCGTGTGACTTTCAATAACCAACGTAGTTACCTGAAAGCAGTCACTGCTGCT TTTGTGGAGATCAAAACCACCAAGTTTACCAAGAAGGTTCAGATCGACCCCTATCTAGAAGATTCTCTGT GTCTTATCTGCAGTTCACAACCTGGTCCTTTCTTCTGCCGAGATCAGGTCTGCTTCAAGTACTTTTGCCG AAGCTGCTGGCACTGGCGACACAGCATGGAAGGCCTACGCCACCACAGCCCGCTAATGAGGAACCAGAAG AAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR204288 representing NM_001106276
Red=Cloning site Green=Tags(s) MAFSLEEESGRIKDCWDNQEVPALSTCSNANIFRRINAILDDSLDFSKVCTTPINRGIHDQLPDFQDSEE AITSRMLFPTSAQESPRGLPDANGLCLGLQSLSLTGWDRPWSTQDSDSSAQSNTQSVLSMLQNPLGNVLG KTPLSFLSLDPLGSDLDKFPAPSVRGSRLDTRPILDSRSSSPSDSDTSGFSSGSDHLSDLISSLRISPPL PFLSMTGNGPRDPLKMGVGSRMDQEQAALAAVAPSPTSAPKRWPGTSVWPSWDLLGAPKDPFSIEREARL HRQAAAVNEATCTWSGQLPPRNYKNPIYSCKVFLGGVPWDITEAGLVNTFRVFGSLSVEWPGKDGKHPRC PPKGNMPKGYVYLVFELEKSVRALLQACSHDPLSPDGLSEYYFKMSSRRMRCKEVQVIPWVLADSNFVWS PSQRLDPSRTVFVGALHGMLNAEALAAILNDLFGGVVYAGIDTDKHKYPIGSGRVTFNNQRSYLKAVTAA FVEIKTTKFTKKVQIDPYLEDSLCLICSSQPGPFFCRDQVCFKYFCRSCWHWRHSMEGLRHHSPLMRNQK N TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001106276 |
| ORF Size | 1683 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001106276.1, NP_001099746.1 |
| RefSeq Size | 2615 bp |
| RefSeq ORF | 1686 bp |
| Locus ID | 293056 |
| UniProt ID | P0C279 |
| MW | 62.1 kDa |
| Gene Summary | Sequence-specific RNA-binding protein that regulates mRNA cytoplasmic polyadenylation and translation initiation during oocyte maturation, early development and at postsynapse sites of neurons. Binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5'-UUUUUAU-3') within the 3' UTR of mRNAs. In absence of phosphorylation and in association with TACC3 is also involved as a repressor of translation of CPE-containing mRNA; a repression that is relieved by phosphorylation or degradation. Involved in the transport of CPE-containing mRNA to dendrites; those mRNAs may be transported to dendrites in a translationally dormant form and translationally activated at synapses. Its interaction with APLP1 promotes local CPE-containing mRNA polyadenylation and translation activation. Induces the assembly of stress granules in the absence of stress. Required for cell cycle progression, specifically for prophase entry.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN204288 | Cpeb1 (untagged ORF) - Rat cytoplasmic polyadenylation element binding protein 1 (Cpeb1), (10 ug) |
CNY 7,130.00 |
|
| RR204288L3 | Lenti ORF clone of Cpeb1 (Myc-DDK-tagged ORF) - Rat cytoplasmic polyadenylation element binding protein 1 (Cpeb1), (10 ug) |
CNY 9,120.00 |
|
| RR204288L4 | Lenti ORF clone of Cpeb1 (mGFP-tagged ORF) - Rat cytoplasmic polyadenylation element binding protein 1 (Cpeb1), (10 ug) |
CNY 9,980.00 |
