Neu3 (NM_054010) Rat Tagged ORF Clone
CAT#: RR204262
- TrueORF®
Neu3 (Myc-DDK-tagged ORF) - Rat sialidase 3 (membrane sialidase) (Neu3), (10 ug)
"NM_054010" in other vectors (3)
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CNY 3,990.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR204262 representing NM_054010
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGAAGTTTCATCCTGCTCCCTCCGCAGCACTCTGTTCCAGCAGGAAGAACAGAACAGGATTACCT ACCGGATCCCAGCACTGCTGTACATTCCTCCCACCCACACCTTCCTGGCCTTCGCAGAGATGCGTACCTC AAGCAGAGATGAGGATGCTGTCTACCTGGTGTTCAGACGAGGGGTGATGAAGGGGTGCTCTGTTGAGTGG GGCCCCCAACAGCCACTGATGGAGGCCACATTACCTGGGCATCGCACCATGAGCCCCTGCCCTGTGTGGG AGAAGAATACTGGCCGTGTATACCTGTTTTTCATCTGCGTGCAGGGCCATGTTAGTGAGCGCTGGCAGCT TCTGTGGGGCAGGAATGCTGCCCGTCTCTGCTTCCTTTACAGTGAGGACTCTGGGTGCTCGTGGGGTGAA GTGAAAGATTTGACTGAGGAAGTCGTTGGCTCAGAGATGAAGCACTGGGCCACGTTTGCTGTGGGCCCAG GTCATGGCATCCAGCTGCAGTCGGGGAGGCTGCTCATTCCCGCCTATGCCTACTTGATATCATGCTGGTT TCTCTGCTTTCCATGTTCAGTCAAGCCCCATTCCCTAATGTTCTACAGTGATGACTTAGGAGTCACATGG CACTGTGGCAAGTTCATTAAGCCCCAGGTGACAGGGGAGTGCCAAGTGGCAGAAGTGCCTGGGAAGGCTG GTAACCTTGTGCTCTACTGCAGTGCCCGAACACCAAACAAATTCCGAGCAGAGGCTTTTAGCACTGATAG TGGTGACTGCTTTCAGAAACCAACCCTGAACCAACAACTCTGCGAGCCTCGTGGTGGCTGCCAAGGTAGT GTAGTGAGCACCCGGCCTTTGAAGATGCCATACACATGTCAAGACTCAAGTGGTAAAGATGTTCCCTCTA CTCAGAAATGCCCTCTGATGGACAGATCTCTGGAGGTGGAGGAAGGAGCTGGAGCACCATCAGGGACATG GCTCTTGTACTCACATCCAACTAACAAGAAGAAGAGAATGAACCTAGGCATCTACTACAACCAAAACCCC TTGGAGGTGAACTACTGGTCCCGCCCGTGGATCTTGAACCGTGGGCCCAGTGGCTACTCTGACCTGGCTG TGGTAGAAGGACAGGGCTTGTTCGCGTGCTTATTTGAGTGTGGGGAGAGGCATGAGGATGAGAAGATTGA CTTTTGTCTGTTTTCAGACCAAGAGGTCCTGAGCTGTGACGACTGTACCAGCCCTAGTAGCAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR204262 representing NM_054010
Red=Cloning site Green=Tags(s) MEEVSSCSLRSTLFQQEEQNRITYRIPALLYIPPTHTFLAFAEMRTSSRDEDAVYLVFRRGVMKGCSVEW GPQQPLMEATLPGHRTMSPCPVWEKNTGRVYLFFICVQGHVSERWQLLWGRNAARLCFLYSEDSGCSWGE VKDLTEEVVGSEMKHWATFAVGPGHGIQLQSGRLLIPAYAYLISCWFLCFPCSVKPHSLMFYSDDLGVTW HCGKFIKPQVTGECQVAEVPGKAGNLVLYCSARTPNKFRAEAFSTDSGDCFQKPTLNQQLCEPRGGCQGS VVSTRPLKMPYTCQDSSGKDVPSTQKCPLMDRSLEVEEGAGAPSGTWLLYSHPTNKKKRMNLGIYYNQNP LEVNYWSRPWILNRGPSGYSDLAVVEGQGLFACLFECGERHEDEKIDFCLFSDQEVLSCDDCTSPSSN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_054010 |
ORF Size | 1254 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_054010.1, NP_446462.1 |
RefSeq Size | 2307 bp |
RefSeq ORF | 1257 bp |
Locus ID | 117185 |
UniProt ID | Q99PW5 |
MW | 47 kDa |
Gene Summary | enzyme that cleaves sialic acid from glycoproteins and glycolipids [RGD, Feb 2006] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
---|
Neuraminidase activity mediates IL-6 production by activated lupus-prone mesangial cells
,null,
American Journal of Physiology - Renal Physiology
,PubMed ID 29357434
[Neu3]
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Documents
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FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN204262 | Neu3 (untagged ORF) - Rat sialidase 3 (membrane sialidase) (Neu3), (10 ug) |
CNY 3,990.00 |
|
RR204262L3 | Lenti ORF clone of Neu3 (Myc-DDK-tagged ORF) - Rat sialidase 3 (membrane sialidase) (Neu3), (10 ug) |
CNY 6,080.00 |
|
RR204262L4 | Lenti ORF clone of Neu3 (mGFP-tagged ORF) - Rat sialidase 3 (membrane sialidase) (Neu3), (10 ug) |
CNY 6,650.00 |