Kif20b (NM_001107609) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Mphosph1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR204124 representing NM_001107609 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAATCTCATTTGAATCAAGACGGAGTGCCTAGACCATCTTATGTTTTTAGTGCTGACCCAATTGCAA GGCCTTCGGAAATCAATTTTGATGGCGTTAAGCTTGATCTCTCTCATGAGTTTTCCTTAGTTGCCGCAAA CCCTGGGGCAAACAGTTTGGAATCTAAAAATTACCTCCAGGTTTGTCTTCGAATAAGACCATTTACACAG TCGGAAAAAGGACACGAGGCTGAGGGCTGTGTGCAAGTTCTGGATTCACAGACTGTTCTGCTGAAAGACC CTCAAAGCATCCTTGGCCACTTAAGTGAGAAGAGCTCCGGACAGATGGCACAGAAATTCAGCTTTTCTAG GGTTTTTGGACCCGAAACTTCACAGAAAGAGTTCTTTCAGGGTTGCATTATGCAGCCAGTAAAAGACCTC TTAAAAGGGCACAGTCGACTGATCTTCACATATGGGCTGACCAATTCTGGAAAAACATATACATTTCAAG GCACAGAAGAAAACATTGGCATTCTGCCTAGAACACTGAATGTATTGTTTGATAGTCTTCAGGAAAGACT CTATACAAAGATGAGCTTCAAACCACATAGATGTAGAGAATACCTACAGTTGTCATCTGATCAAGAGAAA GAGGAAAGTGCTAACAAAAATACATTGCTGCGACAAATTAAAGAGGTTACCATACATAATGACAGTTATG ACATTCTTTGTGGACGCTTAACAAACTCTTTGACTATACCAGAATTTGAAGAAACCATGAACAATTGTGA ACAATCTAGTCTCAATGTGGATAATATAAAATATTCAGTGTGGGTTTCTTTCTTTGAGATTTACAATGAA AGTATTTATGATTTGTTTGTTCCTGTGTCCTCTAAGTTGCAAAAGAGAAAAATGCTACGCCTTTCCCAAG ATGTAAAGGGCTATTCTTTTATAAAAGATCTGCAGTGGATCCAGGTATCTGATTCTAAAGAAGCATATAG ACTCTTAAAACTAGGAGTGAAGCATCAGAGTGTTGCCTTCACAAAACTGAACAATGCTTCTAGCAGAAGT CACAGCATATTCACTATTAGAATATTACAGATAGAAGACTCGGAAATACCTCGTGTAACACGAGTTAGTG AATTGTCGCTATGTGATCTCGCTGGTTCAGAACGAAGCATGAAGACGCAAAGCGAAGGAGAAAGGCTAAG AGAGGCTGGGAATATCAACACTTCTTTACTGACTCTGGGAAAGTGCATCAGTGTTCTGAAGAACAGTGAC AAGTCCAAGATTCAACAACATGTGCCTTTTCGAGAAAGTAAACTGACCCACTATTTCCAAAGCTTTTTTA CTGGAAAGGGGAAAATATGTATGATCATCAATATTAGTCAGTGTTGTTCTGCCTATGATGAGACACTCAA TGTACTGAAGTTCTCAACTGTTGCACAAAAGGTCTATGTTCCAGATACTTTAAGTTCTTCTCAAGAGAAA TCGTTTGGGTCTACCAAATCTTTACAAGATGTATCCCTAGGCAGTAATTTGGATAATAAAATACTAAATG TAAAAAGAAAAACTGTTTCATGGGAAAATAGTCTGGAAGATGTGGTTGAAAATGAAGATTTGGTCGAGGA CTTAGAAGAAAATGAAGAAACACAAAACATGGAAACTGAACTTACAGATGAAGATAGTGATAAGCCGTTA GAGGAAGGCGGAGTCTGCGCAGGTCACGGGAAGAACAAGAAACTGTTGGATTTAATAGAAAACTTGAAAA AAAGACTAATAAATGAAAAAAAAGAAAAGTTAACATTGGAATTTAAAATCCGAGAAGAAGTGACACAGGA ATTCACTCAGTATTGGAGCCAAAGGGAAGCTGATTTTAAGGAAACACTTCTTCATGAACGGGAGATATTA GAAGAAAATGCTGAACGTCGCTTAGCAATTTTCAAAGATTTGGTTGGAAAACCTGGTGAGAGTCAAGATG AACCAGCAAGCAGATTTTGTACCATGGAACTTGAAACTGAAGAGGCTATTGCTTGTTTGCAGCTAAAGTA TAATCAAGTTAAAGCAGAATTAGCTGAAACTAAAGAAGAATTAATTAAAGCCCAAGAAGAGCTAAAAAAT AAAGAGAGTGACTCTCTGGTTCAGGCACTCAAGACATCAAGTAAGAGCTTGTTAACTTCAGGTCAGATAG TTACCAAGCTGGTTTCAGTACTTGGAGGTGAAGGCGGGAGAATCAATAATATCCAGGATATAAAGCAGGA AAATACCGTAACATGTAAAGTTGATACATCTTTAATCAGCAATAAGTCCACTGGTAATGAAACAACTGAA ATGCCCAAGAAGAGTAGAACCCAGACACATTCAGAAAGGAAAAGATTAAATGAAGATGGACTTCAGCTTG GTGAACCACCAGCAAAGAAAGGGCTTATACTTATTAGTCCACCCATCACTGAAGACCAAGACAAAAGGGA AGAAATGCAACAGTCTGTTTCAGAAGGTGCAGAAGAAGACAGTAGAGTTTTGCAGGAAAAGAATGAAGAG CTAAAAAGACTTTTAACTATTGGTGAGAATGAACTCAGAAATGCAAAGGAGGAAAAGGCAGAATTAAATA AACAGGTTGTGAGTTTGCAGCAGCAACTTTGTTTCTTTGAAGAAAAGAACTCATCCCTGAGGGCAGAGGT AGAGCAAATCCAGGCCAGTTATGACCTTGCAGCTGCTGAGCTGCATACTCAGAGAGCTGTGAACCAGGAG CAGAAGGACAGGATCCTGCAATTGTCAGGGAAGATGGAAACTGCGGCAAGACGTATTGAAAGCAATGTTT CACAGATAAAGCAAATGCAAACAAAAATAGATGAACTGCGATCTCTTGATTCACCTTCTCATATTTCAAA AATAGATTTACTCAATCTCCAGGATCTATCAAGTGGTGCTAATTTGCTGAACACATCACAACAGCTTCCA GGTAGTGACCTACCGAGCACATGGGTTAAAGAGTTTCATACTCAGGAGCTCAGCAGGGAAAGTTCCTTCC ACTCTAGCATTGAAGCCATCTGGGAAGAATGTAAAGAGATTGTGAAGGCTTCCTCCAAAAAGTCTCATCA GATTCAGGGACTAGAAGAACTAATTGAAAAATTACAGGTGGAAGTAAAAAACTGCAGGGATGAGAACAGT GAGCTTCGAGCAAAGGAGAGTGAGGATAAGAATCGAGACCAGCAGCTGAAGGAGAAAGAAAGTCTAATCC AGCAGCTTAGAGAAGAACTTCAGGAAACCACTGTCAGTCTCCGTGTCCAAGTACAGCTAGTAGCGGAAAG AGAGCAAGCTCTTTCAGAGCTTTCCCGGGATGTTACCTGCTATAAGGCAAAAGTAAAGGACCTTGAAGTG ATGGTAGAGACCCAGAAAGAGGAATGTAAGCGCTTAGCTGAGTTAGAGCAGAGCATTTTAGAGAAGGAAT CTGCCATCCTAAAGCTGGAAGCGAGTCTAAAGGAATTGGAAGCAAAGCACCAGGATCACATCAGAAGTAC CACACATTTAAATGCAGAGGAAGTCAAGTTCAGAGAAGAAATTACACAGTTGGCGAATAATTTGCATGAT ACAAAACAGTTACTTCAGTCAAAGGAAGAAGAAAATGAAATTAGCAGACAAGAGACAGAAAAGTTGAAAG AGGAGCTTGCTGCAAACTCTGTTCTTACCCAGAATCTCCAAGCAGACCTTCAGCGGAAGGAGGAAGATTG TGCTGAGCTAAAGGAGAAGTTCACTGATGCCAAAAAGCAGATCGAGCAGGTACAGAGGGAGGTATCTGTA ATGCGTGATGAAGAGAAGTCGTTGAGGACTAAAATTAATGAACTGGAGAAAAAGAAAAACCAATATTCTC AGGAAATAGATATGAAACAACGAACCATTCAGCAACTTAAGGAGCAGTTAAGTAATCAGAAAATGGAAGA AGTTGTACAACAGTATGAGAAAGTGTGCAAAGATCTCAGCGTTAAGGAGAAGCTGATTGAAGCTATGCGA CTGACACTAGTGGAGCAGGAGCAAACTCAGGCAGAGCAGGACAGGATGCTTGAGGCTAAGTCACAGGAAG CTGACTGGCTAGCCGGAGAATTGGATACATGGAAAGACAAATTCAAAGATCTGGAGACTAGAAGTAATCA AAAGGTAACTACAGAGGCCATGGAGGACAGCGACGTGCTTAGTGAGAAGTTCAGGAAGCTTCAGGACGAG CTGCAGGAATCTGAAGAGAAACATAAAGCTGATAGAAAGAAGTGGTTAGAAGAAAAAGCGGTTCTTACAA CTCAAGCAAAAGAAGCAGAGACTCTACGAAACAGGGAGATGAAAAAATATGCTGAGGACAGGGAGCGTTG TCTGAAGCTGCAGAATGAAGTGGAAACACTGACAGCACAGCTGGCGGAGAAAACTGGTGAGCTTCAGAAG TGGAGAGAAGAGCGGGACCAGCTGGTCACAGCTGTGGAGACACAGATGCAGGCTCTGCTCTCCAGCAGCA AGCATAAGGACGAAGAGATCCAGCAACTGAGAAAGGCCGTGGCAAAGAGCACGGGGACAGAAAACCAGAC CATGAATCTCAAGCCTGAATGTAATGATTCAGTCGATCTTGGTGGAGTTGAAACTGAACTTCAGTCAACA AGTTTTGAAATTTCTAGGAATACAGCAGAGGATGGATCTGTAGTTCTTGACTCTTGTGAAGTGTCAACAG AAAATGTACAAAGTACTCGATTTCCAAAGCCTGAACTGGAGATTCAGTTTACACCTCTGCAGCCAAACAA AGTGGCAGTGAAGCACCCTGGTTGTGCCACACCAGTCACAATTAAGATCCCCAAGGCACGGAAGAGGAAG AGTGGCGAGGTGGAGGAGGACTTGGTGAAATGTGAAAATAAGAAGAATTCCACACCCAGAAGTAATGTGA AGTTTCCCGTTTCAGAGCATAGAAATTCCTCTTTCAAAAAGGAACAAAAGGTTTTCTTATGTCCGTCATC TAAGAAGACATACTCCTTACGGAGCCAAGCATCTACGGTTGGTGCGAATGTAGCCTCTAAAAAAAAAGAG GGAACACTACAGAAATTTGGAGACTTCTTACAACATTCTCCAACAATCCTTCAATCAAAAGCAAAAAAGA TAATTGAAACAATGAGTTCCCCAAAGTTATCAACTGTAGAAGTAAGTAAAGAAAATGTATCCCAGCCTAA AAAAGCCAAACGGAAATTGTACAGAAATGAAATTTCATCTCCCATCAACATATCTGGCCAAGTGATTTTA ATGGAACAGAAAGCAAAAGAAACTGATCATCAGATTCTCAAACGACGGCTTCGAACACGAACAGCCAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR204124 representing NM_001107609 Red=Cloning site Green=Tags(s) MESHLNQDGVPRPSYVFSADPIARPSEINFDGVKLDLSHEFSLVAANPGANSLESKNYLQVCLRIRPFTQ SEKGHEAEGCVQVLDSQTVLLKDPQSILGHLSEKSSGQMAQKFSFSRVFGPETSQKEFFQGCIMQPVKDL LKGHSRLIFTYGLTNSGKTYTFQGTEENIGILPRTLNVLFDSLQERLYTKMSFKPHRCREYLQLSSDQEK EESANKNTLLRQIKEVTIHNDSYDILCGRLTNSLTIPEFEETMNNCEQSSLNVDNIKYSVWVSFFEIYNE SIYDLFVPVSSKLQKRKMLRLSQDVKGYSFIKDLQWIQVSDSKEAYRLLKLGVKHQSVAFTKLNNASSRS HSIFTIRILQIEDSEIPRVTRVSELSLCDLAGSERSMKTQSEGERLREAGNINTSLLTLGKCISVLKNSD KSKIQQHVPFRESKLTHYFQSFFTGKGKICMIINISQCCSAYDETLNVLKFSTVAQKVYVPDTLSSSQEK SFGSTKSLQDVSLGSNLDNKILNVKRKTVSWENSLEDVVENEDLVEDLEENEETQNMETELTDEDSDKPL EEGGVCAGHGKNKKLLDLIENLKKRLINEKKEKLTLEFKIREEVTQEFTQYWSQREADFKETLLHEREIL EENAERRLAIFKDLVGKPGESQDEPASRFCTMELETEEAIACLQLKYNQVKAELAETKEELIKAQEELKN KESDSLVQALKTSSKSLLTSGQIVTKLVSVLGGEGGRINNIQDIKQENTVTCKVDTSLISNKSTGNETTE MPKKSRTQTHSERKRLNEDGLQLGEPPAKKGLILISPPITEDQDKREEMQQSVSEGAEEDSRVLQEKNEE LKRLLTIGENELRNAKEEKAELNKQVVSLQQQLCFFEEKNSSLRAEVEQIQASYDLAAAELHTQRAVNQE QKDRILQLSGKMETAARRIESNVSQIKQMQTKIDELRSLDSPSHISKIDLLNLQDLSSGANLLNTSQQLP GSDLPSTWVKEFHTQELSRESSFHSSIEAIWEECKEIVKASSKKSHQIQGLEELIEKLQVEVKNCRDENS ELRAKESEDKNRDQQLKEKESLIQQLREELQETTVSLRVQVQLVAEREQALSELSRDVTCYKAKVKDLEV MVETQKEECKRLAELEQSILEKESAILKLEASLKELEAKHQDHIRSTTHLNAEEVKFREEITQLANNLHD TKQLLQSKEEENEISRQETEKLKEELAANSVLTQNLQADLQRKEEDCAELKEKFTDAKKQIEQVQREVSV MRDEEKSLRTKINELEKKKNQYSQEIDMKQRTIQQLKEQLSNQKMEEVVQQYEKVCKDLSVKEKLIEAMR LTLVEQEQTQAEQDRMLEAKSQEADWLAGELDTWKDKFKDLETRSNQKVTTEAMEDSDVLSEKFRKLQDE LQESEEKHKADRKKWLEEKAVLTTQAKEAETLRNREMKKYAEDRERCLKLQNEVETLTAQLAEKTGELQK WREERDQLVTAVETQMQALLSSSKHKDEEIQQLRKAVAKSTGTENQTMNLKPECNDSVDLGGVETELQST SFEISRNTAEDGSVVLDSCEVSTENVQSTRFPKPELEIQFTPLQPNKVAVKHPGCATPVTIKIPKARKRK SGEVEEDLVKCENKKNSTPRSNVKFPVSEHRNSSFKKEQKVFLCPSSKKTYSLRSQASTVGANVASKKKE GTLQKFGDFLQHSPTILQSKAKKIIETMSSPKLSTVEVSKENVSQPKKAKRKLYRNEISSPINISGQVIL MEQKAKETDHQILKRRLRTRTAK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001107609
ORF Size	5319 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001107609.2, NP_001101079.2
RefSeq Size	5567 bp
RefSeq ORF	5322 bp
Locus ID	309523
MW	202.7 kDa

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