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Fa2h (NM_001135583) Rat Tagged ORF Clone
CAT＃: RR204101
- TrueORF®
Fa2h (Myc-DDK-tagged ORF) - Rat fatty acid 2-hydroxylase (Fa2h), (10 ug)
"NM_001135583" in other vectors (3)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | RGD1310347; Wdr59 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR204101 representing NM_001135583
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCCCGCTCCGCCCCCCGCCGCCTCCTTCACGTCCGCCGAGGTCCAGCGGCGCCTGGCGGCCGGTG CCTGCTGGGTCCGTCGCGGGGCCAGTCTCTACGACCTCACCGGCTTCGTGCGGCATCATCCGGGGGGCGA GCAGCTGCTGCTGGCGCGGGCTGGCCAGGACATCAGTGCCGACCTGGATGGGCCACCTCATAAACACTCG GACAACGCTCGCCGCTGGCTGGAGCAGTACTATGTGGGCGAACTGCGAGCGGACCCGCAGGATCCCACAG AGAACGGTGCTGGAGCCCCTGCAGAGACTCAGAAGACAGATGCTGCTATTGAGCCACAGTTCAAAGTGGT AGACTGGGACAAGGACCTGGTGGACTGGCAGAAACCTCTCCTGTGGCAGGTGGGCCACTTGGGGGAAAAG TATGATGAGTGGGTTCACCAACCTGTGGCCAGGCCCATTCGCCTCTTCCACTCAGACCTCATCGAGGCCT TCTCCAAGACTGTCTGGTATAGTGTCCCCATCATCTGGGTGCCCCTGGTGCTGTACCTCAGCTGGTCCTA CTACCGAACCCTCACCCAGGACAACATCCGGCTTTTTGCATCATTCACAAGAGATTATTCACTTGTGGTG CCAGAGTCGGTGTTCATAGGCCTCTTCGTGTTGGGCATGCTTATCTGGACTCTTGTGGAGTACCTCATCC ACCGCTTCCTGTTCCACATGAAGCCCCCCAGCAACAGCCATTACCTCATCATGCTGCATTTTGTCATGCA CGGACAGCACCACAAGGCACCCTTTGATGGCTCCCGTCTGGTCTTCCCCCCAGTGCCAGCCTCTGTAGTG GTGGCCTTCTTCTATGTGTTCCTGCGGCTCATTCTGCCTGAGGCCGTGGCAGGCATCCTCTTTGCTGGGG GTCTTCTGGGCTATGTCCTCTATGACATGACCCATTACTACCTGCACTTTGGCTCACCTCACAAGGGCTC CTATCTGTACAACATGAAGGCCCACCATGTCAAGCATCACTTCGAATACCAGAAATCAGGGTTCGGCATC AGCACTAAACTGTGGGATTACTTTTTCCACACCCTCATTCCAGAGGAAGCCGACCCGAAGATGCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR204101 representing NM_001135583
Red=Cloning site Green=Tags(s) MAPAPPPAASFTSAEVQRRLAAGACWVRRGASLYDLTGFVRHHPGGEQLLLARAGQDISADLDGPPHKHS DNARRWLEQYYVGELRADPQDPTENGAGAPAETQKTDAAIEPQFKVVDWDKDLVDWQKPLLWQVGHLGEK YDEWVHQPVARPIRLFHSDLIEAFSKTVWYSVPIIWVPLVLYLSWSYYRTLTQDNIRLFASFTRDYSLVV PESVFIGLFVLGMLIWTLVEYLIHRFLFHMKPPSNSHYLIMLHFVMHGQHHKAPFDGSRLVFPPVPASVV VAFFYVFLRLILPEAVAGILFAGGLLGYVLYDMTHYYLHFGSPHKGSYLYNMKAHHVKHHFEYQKSGFGI STKLWDYFFHTLIPEEADPKMQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001135583 |
| ORF Size | 1116 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001135583.1, NP_001129055.1 |
| RefSeq Size | 2506 bp |
| RefSeq ORF | 1119 bp |
| Locus ID | 307855 |
| UniProt ID | Q2LAM0 |
| MW | 42.7 kDa |
| Gene Summary | Catalyzes stereospecific hydroxylation of free fatty acids at the C-2 position to produce (R)-2-hydroxy fatty acids, which are building blocks of sphingolipids and glycosphingolipids common in neural tissue and epidermis (By similarity). Plays an essential role in the synthesis of galactosphingolipids of the myelin sheath (PubMed:17901466). Responsible for the synthesis of sphingolipids and glycosphingolipids involved in the formation of epidermal lamellar bodies critical for skin permeability barrier (By similarity). Participates in the synthesis of glycosphingolipids and a fraction of type II wax diesters in sebaceous gland, specifically regulating hair follicle homeostasis (By similarity). Involved in the synthesis of sphingolipids of plasma membrane rafts, controlling lipid raft mobility and trafficking of raft-associated proteins (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN204101 | Fa2h (untagged ORF) - Rat fatty acid 2-hydroxylase (Fa2h), (10 ug) |
CNY 3,990.00 |
|
| RR204101L3 | Lenti ORF clone of Fa2h (Myc-DDK-tagged ORF) - Rat fatty acid 2-hydroxylase (Fa2h), (10 ug) |
CNY 6,080.00 |
|
| RR204101L4 | Lenti ORF clone of Fa2h (mGFP-tagged ORF) - Rat fatty acid 2-hydroxylase (Fa2h), (10 ug) |
CNY 6,650.00 |
