Gulp1 (NM_001013171) Rat Tagged ORF Clone

CAT#: RR204040

  • TrueORF®

Gulp1 (Myc-DDK-tagged ORF) - Rat GULP, engulfment adaptor PTB domain containing 1 (Gulp1), (10 ug)



  "NM_001013171" in other vectors (3)

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CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Synonyms CED-6
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR204040 representing NM_001013171
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACCGGGCTTTCAGCAGGAAGAAAGACAAAACATGGATGCATATGCCTGAAGCCTTATCAAAACATT
ACGTTCCCTATAATGCAAAGTTTCTTGGCAGTACTGAAGTGGAGCAGCCAAAGGGAACAGAAGTTGTGAG
AGATGCTGTCAGGAAGCTGAAGTTTGCAAGACACATCAAGAAATCTGAAGGCCAGAAGATTCCTAAAGTT
GAATTGCAAATATCAATTTATGGAGTGAAAATTCTCGAACCAAAAACAAAGGAAGTTCAACACAACTGCC
AGCTTCATAGAATATCATTTTGTGCAGATGATAAAACCGACAAGAGGATTTTCACCTTCATATGCAAAGA
TTCTGAGTCAAACAAACATTTGTGTTTTGTGTTTGACAGCGAAAAGTGTGCTGAAGAAATTACATTAACA
ATTGGCCAAGCATTTGACTTAGCATACAGGAAATTTCTAGAATCTGGAGGAAAAGATGTGGAAACAAGAA
AACAGATTGCTGGAATGCAGAAAAGAATCCAAGACTTAGAAACAGAAAATATGGAACTTAAAAATAAAGT
ACAAGATTTAGAAAACCGGTTGAGAACAACTCAAGTGTCCTCGACTCCAGCTGGTGGTGTGACAGTGACT
TCTCCTTCCACTGACATCTTTGACATGATTCCATTTTCCCCAATATCACACCCGTCTCCAACCTCTGCTG
GCAATGGCACACAGCTACCACCCATACCTAGCAGATCTTCAGAGATCAAACGGGACCTTTTTGGAGCTGA
ACCTTTTGACCCATTTAACTGTGGAGCAGGGGACTTTCCTCCTGATATTCAGTCCAAATTGGATGAGATG
CAGGAGGGGTTCAAAATGGGACTAACTCTTGAAGGAACAGTATTTTGTCTCGACCCATTAGACAGCAGGT
GC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR204040 representing NM_001013171
Red=Cloning site Green=Tags(s)

MNRAFSRKKDKTWMHMPEALSKHYVPYNAKFLGSTEVEQPKGTEVVRDAVRKLKFARHIKKSEGQKIPKV
ELQISIYGVKILEPKTKEVQHNCQLHRISFCADDKTDKRIFTFICKDSESNKHLCFVFDSEKCAEEITLT
IGQAFDLAYRKFLESGGKDVETRKQIAGMQKRIQDLETENMELKNKVQDLENRLRTTQVSSTPAGGVTVT
SPSTDIFDMIPFSPISHPSPTSAGNGTQLPPIPSRSSEIKRDLFGAEPFDPFNCGAGDFPPDIQSKLDEM
QEGFKMGLTLEGTVFCLDPLDSRC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001013171
ORF Size 912 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001013171.2, NP_001013189.1
RefSeq Size 2835 bp
RefSeq ORF 915 bp
Locus ID 314543
UniProt ID Q5PQS4
MW 34.3 kDa
Gene Summary May function as an adapter protein. Required for efficient phagocytosis of apoptotic cells. Modulates cellular glycosphingolipid and cholesterol transport. May play a role in the internalization and endosomal trafficking of various LRP1 ligands, such as PSAP. Increases cellular levels of GTP-bound ARF6 (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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