Upf2 (NM_001108421) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR203833 representing NM_001108421 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGCTGAGCGGAAAAAGTCAGCAAGTATGGAGGAAAAAGAATCTTTACTAAACAACAAGGAAAAAG ACTGCAGTGAGAGGCGGCCAGTGAGCAGCAAGGAGAAATCAAAAGACGATCTCAAGATCACTGCCAAGAA GGAGGTCAGTAAGGTCCCAGAAGACAAAAAGAAGAAACTGGAAGAAGATAAGAGAAAAAAGGAAGACAAG GAACGTAAGAAAAAAGAGGAAGAGAAGGTGAAGGCAGAGGAAGAGTTGAAGAAGAAGGAAGAGGAAGAAA AGAAAAAACAGGAAGAGGAAGAGAGGAAAAAGCAGGAGGAGCAGGCCAAGCGGCAGCAGGAAGAAGCAGC TGCCCAGCTGAAAGAGAAAGAAGAATCTCTTCAGCTTCATCAGGAGGCGTGGGAACGCCATCAACTACGA AAGGAACTCCGTAGCAAAAACCAAAATGCTCCAGACAACCGACCAGAGGAAAATTTCTTTAGCCGCCTAG ATTCGAGCTTAAAGAAAAATACTGCTTTTGTCAAGAAACTAAAAACAATTACAGAACAACAGAGAGACTC CTTGTCTCATGATTTTAATGGCCTGAATTTAAGCAAATATATTGGGGAAGCCGTAGCTTCCATTGTGGAA GCAAAATTAAAAATCTCTGACGTGAACTGTGCCGTGCACCTCTGCTCTCTCTTTCACCAGCGGTACGCTG ACTTTGCACCCTCACTTCTTCAGGTGTGGAAAAAACATTTTGAAGCAAGGAAAGAGGAGAAAACGCCCAA CATTACCAAGCTAAGAACTGACTTGCGTTTCATTGCAGAATTGACAATAGTGGGAATCTTCACTGACAAG GAAGGTCTTTCCTTAATCTATGAACAGCTAAAAAGTATTATTAATGCTGATCGAGAATCCCACACTCATG TGTCTGTGGTGATTAGTTTCTGTCGTCATTGTGGAGATGATATTGCTGGACTTGTACCGAGGAAAGTAAA GAGTGCTGCAGAGAAGTTTAATTTGAACTTTCCTCCTAGTGAGATAATTAGCCCAGAGAAGCAGCAGCCT TTCCAGAATCTTTTAAAAGAGTACTTTACGTCTCTGACCAAACACCTGAAAAGGGACCACAGGGAGCTTC AGAATACTGAGAGGCAAAACAGGCGTATTCTGCACTCTAAAGGCGAGCTAAGTGAAGACAGGCATAAACA GTATGAAGAGTTCGCCATGTCTTACCAGAAGCTGCTGGCAAATTCTCAATCCTTAGCCGACCTTTTGGAT GAAAATATGCCAGATCTTCCTCAGGACAAACCGACACCAGAAGAGCATGGACCTGGAATCGATATATTTA CACCTGGAAAACCTGGAGAATATGACTTAGAAGGTGGCATATGGGAAGACGAAGACGCTCGGAATTTTTA TGAAAACCTCATCGATTTGAAAGCATTTGTTCCAGCCATCTTGTTTAAAGACAATGAAAAAAGTCAGAAC AAAGATTCTAGCAAAGATGATTCCAAAGAGGCAAAAGAACCAAAGGATAATAAGGAAGCATCAAGTCCCG ATGATTTGGAACTTGAGTTGGAGAATTTAGAAATTAATGATGACACACTGGAGTTAGAAGGTGGAGATGA AGCTGAAGATCTTACAAAGAAACTCCTTGATGAACAAGAACAAGAAGATGAAGAAGCCAGCACTGGATCT CACCTCAAACTCATAGTGGATGCTTTCCTCCAGCAGCTGCCCAACTGTGTCAACCGAGACCTGATTGACA AGGCAGCCATGGACTTTTGCATGAACATGAACACAAAGGCAAACAGGAAGAAGTTGGTTCGGGCGCTCTT CATAGTTCCTAGGCAAAGGTTGGATTTGCTCCCATTTTATGCAAGATTGGTTGCTACATTGCATCCCTGC ATGTCTGATGTAGCAGAGGATCTTTGTTCCATGCTGAGGGGGGATTTCAGATTTCATGTTCGAAAAAAGG ACCAGATCAACATTGAAACAAAGAATAAAACTGTCCGTTTTATTGGAGAACTAACCAAGTTTAAGATGTT CACCAAAAATGATACACTGCATTGTCTAAAGATGCTTCTGTCAGACTTTTCTCATCATCATATTGAAATG GCATGTACCCTACTGGAGACCTGTGGACGGTTTCTTTTCCGATCTCCAGAATCACACCTGAGGACCAGTG TGCTTTTGGAGCAAATGATGAGAAAGAAGCAAGCAATGCACCTGGACGCGCGCTACGTTACAATGGTGGA GAACGCGTATTACTACTGCAACCCACCACCAGCCGAGAAGACAGTGAGAAGGAAGCGGCCTCCTCTTCAG GAGTATGTCCGCAAACTTCTGTACAAGGATCTCTCTAAGGTCACCACGGAAAAGGTTTTGAGACAGATGC GAAAGTTGCCCTGGCAGGACCAAGAAGTTAAAGACTATGTCATTTGTTGTATGATAAACATTTGGAATGT GAAATACAATAGTATTCACTGTGTAGCCAACCTCCTAGCAGGACTAGTGCTTTACCAGGAGGATGTTGGG ATCCACGTTGTGGACGGAGTACTTGAAGATATTCGATTGGGAATGGAGGTTAATCAACCTAAATTTAACC AGAGGCGCATCAGTAGTGCCAAGTTCTTAGGAGAACTTTATAATTACAGAATGGTCGAATCGGCTGTTAT TTTCAGAACTCTTTATTCTTTTACTTCATTTGGTGTCAACCCTGACGGGTCTCCAAGTTCCCTGGACCCT CCTGAGCATCTGTTTAGAATTAGACTAGTCTGCACTATTTTGGATACCTGTGGCCAGTACTTTGACAGAG GTTCCAGTAAAAGAAAACTTGACTGCTTCCTTGTATATTTTCAGCGTTATGTTTGGTGGAAGAAAAGTTT GGAGGTTTGGACAAAAGACCATCCATTTCCTATTGATATAGATTACATGATCAGTGATACACTAGAACTG CTAAGACCAAAGATCAAACTCTGTAATTCTCTGGAAGAATCCATCAGGCAGGTACAAGACTTGGAACGAG AATTCTTAATAAAACTAGGCCTAGTAAATGACAAAGAGTCCAAAGATTCTATGACAGAAGGGGAAAATCT TGAAGAGGATGAAGAAGAAGAAGAAGGAGGAGCTGAGACAGAGGAACAGTCTGGAAATGAGAGTGAAGTA AACGAGCCAGAAGAAGAAGAGGGTTCTGATGATGAAGACGAGGGAGAGGAGGAGGAAGAAGAGAATACAG ATTACCTCACAGATTCCAACAAGGAGAACGAGACTGATGAAGAGAATGCTGAGGTGATGATTAAGGGAGG CGGACTGAAGCATGTTCCTTGTGTAGAAGATGAGGACTTCATTCAAGCTCTGGACAAAATGATGCTAGAA AACCTTCAGCGAAGTGGTGAATCTGTGAAAGTACACCAACTTGATGTTGCTATTCCTTTGCATCTCAAAA GCCAGCTGAGGAAAGGGCCCCCACTTGGTGGTGGGGAAGGAGAGACAGAGTCTGCAGACACGATGCCGTT TGTCATGCTGACAAGGAAAGGCAATAAACAGCAGTTTAAGATTCTCAATGTGCCCATGTCATCCCAACTT GCTGCAAATCACTGGAACCAGCAACAAGCAGAACAAGAGGAGAGGATGAGGATGAAAAAGCTCACCTTAG ACATCAACGAACGACAAGAACAAGAAGATTATCAAGAAATGTTGCAGTCTCTCGCACAGCGCCCAGCTCC AGCGAACACTAATCGTGAGCGGCGGCCTCGTTACCAACATCCAAAGGGAGCGCCTAATGCAGATCTAATC TTTAAGACTGGTGGGAGGAGACGT AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR203833 representing NM_001108421 Red=Cloning site Green=Tags(s) MPAERKKSASMEEKESLLNNKEKDCSERRPVSSKEKSKDDLKITAKKEVSKVPEDKKKKLEEDKRKKEDK ERKKKEEEKVKAEEELKKKEEEEKKKQEEEERKKQEEQAKRQQEEAAAQLKEKEESLQLHQEAWERHQLR KELRSKNQNAPDNRPEENFFSRLDSSLKKNTAFVKKLKTITEQQRDSLSHDFNGLNLSKYIGEAVASIVE AKLKISDVNCAVHLCSLFHQRYADFAPSLLQVWKKHFEARKEEKTPNITKLRTDLRFIAELTIVGIFTDK EGLSLIYEQLKSIINADRESHTHVSVVISFCRHCGDDIAGLVPRKVKSAAEKFNLNFPPSEIISPEKQQP FQNLLKEYFTSLTKHLKRDHRELQNTERQNRRILHSKGELSEDRHKQYEEFAMSYQKLLANSQSLADLLD ENMPDLPQDKPTPEEHGPGIDIFTPGKPGEYDLEGGIWEDEDARNFYENLIDLKAFVPAILFKDNEKSQN KDSSKDDSKEAKEPKDNKEASSPDDLELELENLEINDDTLELEGGDEAEDLTKKLLDEQEQEDEEASTGS HLKLIVDAFLQQLPNCVNRDLIDKAAMDFCMNMNTKANRKKLVRALFIVPRQRLDLLPFYARLVATLHPC MSDVAEDLCSMLRGDFRFHVRKKDQINIETKNKTVRFIGELTKFKMFTKNDTLHCLKMLLSDFSHHHIEM ACTLLETCGRFLFRSPESHLRTSVLLEQMMRKKQAMHLDARYVTMVENAYYYCNPPPAEKTVRRKRPPLQ EYVRKLLYKDLSKVTTEKVLRQMRKLPWQDQEVKDYVICCMINIWNVKYNSIHCVANLLAGLVLYQEDVG IHVVDGVLEDIRLGMEVNQPKFNQRRISSAKFLGELYNYRMVESAVIFRTLYSFTSFGVNPDGSPSSLDP PEHLFRIRLVCTILDTCGQYFDRGSSKRKLDCFLVYFQRYVWWKKSLEVWTKDHPFPIDIDYMISDTLEL LRPKIKLCNSLEESIRQVQDLEREFLIKLGLVNDKESKDSMTEGENLEEDEEEEEGGAETEEQSGNESEV NEPEEEEGSDDEDEGEEEEEENTDYLTDSNKENETDEENAEVMIKGGGLKHVPCVEDEDFIQALDKMMLE NLQRSGESVKVHQLDVAIPLHLKSQLRKGPPLGGGEGETESADTMPFVMLTRKGNKQQFKILNVPMSSQL AANHWNQQQAEQEERMRMKKLTLDINERQEQEDYQEMLQSLAQRPAPANTNRERRPRYQHPKGAPNADLI FKTGGRRR SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001108421
ORF Size	3804 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001108421.1, NP_001101891.1
RefSeq Size	4445 bp
RefSeq ORF	3807 bp
Locus ID	361271
MW	147.3 kDa

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