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Atxn1 (NM_012726) Rat Tagged ORF Clone
CAT＃: RR203715
- TrueORF®
Atxn1 (Myc-DDK-tagged ORF) - Rat ataxin 1 (Atxn1), (10 ug)
"NM_012726" in other vectors (3)
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CNY 10,170.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Sca1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR203715 representing NM_012726
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAATCTAACCAAGAGCGGAGCAATGAATGCCTGCCTCCCAAGAAACGTGAGATCCCCGCCACCAGTC GGCCCTCCGAGGAGAAGGCCACTGCTCTGCCCAGCGACAACCACTGCGTGGAGGGTGTGGCATGGCTCCC CAGCACCCCTGGCAGCCGCGGCCACGGGGGTGGGCGGCACGGGCCAGCAGGGACTTCCGGGGAACATGGT TTACAAGGAATGGGTTTACATAAAGCACTGTCCGCAGGGCTGGATTACTCCCCACCCAGTGCCCCCAGGT CGGTCCCCACAGCCAACACGCTGCCCACCGTGTACCCTCCTCCTCAGTCAGGGACGCCGGTGTCTCCTGT GCAGTACGCCCACCTATCACATACCTTCCAGTTCATTGGGTCCTCCCAGTATAGTGGGCCTTACGCGGGC TTTATCCCTTCCCAGCTGATCTCCCCACCAGGCAACCCAGTCACCAGTGCGGTGGCCTCGGCTGCAGGGG CCACCACTCCATCACAGCGCTCCCAGCTGGAGGCATATTCCACCCTGCTGGCCAACATGGGCAGTCTGAG CCAGGCACCAGGACACAAGGTTGAGCCCCCTCCGCAGCAGCACCTCGGCAGGGCTGCGGGATTAGTCAAC CCGGGGTCCCCTCCACCTACCCAGCAGAACCAGTACATTCACATTTCCAGCTCTCCGCAGAGCTCCGGGC GGGCAACATCTCCACCCATCCCGGTCCACCTCCATCCCCATCAGACGATGATCCCGCACACGCTCACCCT GGGGCCTTCATCCCAGGTGGTCGTGCAATACAGTGACGCCGGAGGCCACTTTGTTCCTCGAGAGTCCACC AAAAAAGCAGAAAGCAGCAGGTTGCAGCAGGCTATGCAGGCCAAGGAGGTCCTCAATGGGGAGATGGAGA AAAGCCGGAGGTATGGGGCGTCATCTTCTGTGGAGCTGAGCCTGGGGAAGACGAGCAGCAAGTCAGTGCC TCACCCCTATGAGTCCAGGCATGTGGTGGTCCACCCGAGCCCAGCAGACTACAGCAGTCGTGATACCTCC GGGGTCCGTGGATCTGTGATGGTCCTGCCCAACAGCAGCACACCCTCAGCCGACCTGGAGACACAGCAGG CCACACATCGAGAGGCCTCCCCATCCACCCTCAATGACAAGAGCGGTTTGCACCTAGGGAAGCCCGGCCA CAGGTCCTACGCGCTGTCCCCGCACACGGTCATTCAGACCACACACAGCGCATCAGAGCCTCTCCCGGTG GGCCTACCAGCCACGGCCTTCTATGCTGGCGCTCAACCTCCTGTCATCGGCTATCTGAGTAGCCAGCAGC AAGCAATCACCTATGCTGGTGGTCTGCCCCAGCACCTGGTGATCCCAGGTACCCAGCCCCTGCTCATCCC AGTGGGCAGCCCTGACATGGACACACCTGGGGCAGCCTCGGCCATAGTGACGTCATCGCCCCAGTTTGCT GCAGTACCTCACACGTTTGTCACCACCGCCCTGCCCAAGAGCGAGAACTTCAACCCAGAGGCTCTGGTCA CCCAGGCAGCCTACCCAGCCATGGTGCAGGCCCAGATCCACCTGCCGGTGGTACAGTCCGTGGCATCCCC TGCCGCGGCATCACCCACGCTGCCGCCATATTTCATGAAAGGCTCCATCATCCAGCTGGCCAACGGGGAG CTGAAGAAGGTAGAGGATCTGAAGACAGAGGATTTCATCCAGAGTGCAGAGATTAGCAATGACCTCAAGA TCGACTCCAGTACTGTGGAGAGGATCGAGGACAGCCACAGCCCCGGTGTGGCGGTGATACAATTTGCTGT TGGTGAACACCGAGCCCAGGTCAGTGTCGAAGTTTTGGTAGAGTATCCTTTTTTTGTATTTGGACAGGGC TGGTCATCCTGCTGTCCCGAGCGGACCAGCCAGCTCTTTGATCTGCCGTGTTCCAAACTCTCCGTTGGGG ACGTCTGCATCTCGCTCACCCTCAAGAACCTGAAGAATGGCTCTGTTAAAAAGGGCCAGCCCGTGGACCC TGCCAGTGCCCTGCTGAAGCACGCAAAGACCGACAGCCTGGCTGGCAGCAGACACAGATACGCCGAGCAG GAAAACGGAATCAACCAGGGGAGCGCCCAGGTGCTCTCTGAGAACGGCGAACTGAAGTTTCCAGAAAAAA TTGGATTGCCTGCAGCACCCTTCCTCACCAAAATAGAACCGAGCAAGCCCACAGCCACGAGGAAGAGGAG GTGGTCGGCGCCGGAGACCCGTAAACTGGAGAAGTCGGAGGACGAGCCACCTTTGACTCTTCCCAAGCCT TCGCTCATTCCTCAGGAGGTTAAGATCTGCATCGAAGGCCGATCTAACGTGGGCAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR203715 representing NM_012726
Red=Cloning site Green=Tags(s) MKSNQERSNECLPPKKREIPATSRPSEEKATALPSDNHCVEGVAWLPSTPGSRGHGGGRHGPAGTSGEHG LQGMGLHKALSAGLDYSPPSAPRSVPTANTLPTVYPPPQSGTPVSPVQYAHLSHTFQFIGSSQYSGPYAG FIPSQLISPPGNPVTSAVASAAGATTPSQRSQLEAYSTLLANMGSLSQAPGHKVEPPPQQHLGRAAGLVN PGSPPPTQQNQYIHISSSPQSSGRATSPPIPVHLHPHQTMIPHTLTLGPSSQVVVQYSDAGGHFVPREST KKAESSRLQQAMQAKEVLNGEMEKSRRYGASSSVELSLGKTSSKSVPHPYESRHVVVHPSPADYSSRDTS GVRGSVMVLPNSSTPSADLETQQATHREASPSTLNDKSGLHLGKPGHRSYALSPHTVIQTTHSASEPLPV GLPATAFYAGAQPPVIGYLSSQQQAITYAGGLPQHLVIPGTQPLLIPVGSPDMDTPGAASAIVTSSPQFA AVPHTFVTTALPKSENFNPEALVTQAAYPAMVQAQIHLPVVQSVASPAAASPTLPPYFMKGSIIQLANGE LKKVEDLKTEDFIQSAEISNDLKIDSSTVERIEDSHSPGVAVIQFAVGEHRAQVSVEVLVEYPFFVFGQG WSSCCPERTSQLFDLPCSKLSVGDVCISLTLKNLKNGSVKKGQPVDPASALLKHAKTDSLAGSRHRYAEQ ENGINQGSAQVLSENGELKFPEKIGLPAAPFLTKIEPSKPTATRKRRWSAPETRKLEKSEDEPPLTLPKP SLIPQEVKICIEGRSNVGK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012726 |
| ORF Size | 2367 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012726.2, NP_036858.1 |
| RefSeq Size | 4717 bp |
| RefSeq ORF | 2370 bp |
| Locus ID | 25049 |
| UniProt ID | Q63540 |
| MW | 83.5 kDa |
| Gene Summary | human homolog contains an expanded CAG nucleotide repeat region that causes the neurodegenerative disorder spinocerebellar ataxia type 1 (SCA1) [RGD, Feb 2007] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN203715 | Atxn1 (untagged ORF) - Rat ataxin 1 (Atxn1), (10 ug) |
CNY 10,170.00 |
|
| RR203715L3 | Lenti ORF clone of Atxn1 (Myc-DDK-tagged ORF) - Rat ataxin 1 (Atxn1), (10 ug) |
CNY 11,970.00 |
|
| RR203715L4 | Lenti ORF clone of Atxn1 (mGFP-tagged ORF) - Rat ataxin 1 (Atxn1), (10 ug) |
CNY 13,110.00 |
