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Zgpat (NM_001009656) Rat Tagged ORF Clone
CAT＃: RR203531
- TrueORF®
Zgpat (Myc-DDK-tagged ORF) - Rat zinc finger, CCCH-type with G patch domain (Zgpat), (10 ug)
"NM_001009656" in other vectors (3)
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CNY 6,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | RGD1310801 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR203531 representing NM_001009656
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGAGGACAACCTAGAGACTGCCCTGCAGACCTACCGGGCCCAGCTTCAGCAAGTAGAGCTGGCGC TCGGTGCTGGCCTAGACGCTTCTGAGCAGGCGGACCTGCGCCAGCTGCAGGGCGATCTAAAAGAATTGAT CGAGCTCACCGAGGCCAGCCTGTTGTCGGTCAGGAAGAGCAAGCTGCTATCTACAGTGGACCAGGAGCAC CAAGAAGATGCTGAGTACTTGGCTTTCCAGAAGGCTATAGCGGAGGAAGCACCCGTTGACCCCGGCAATG ACTCGAAGACGGTCCCTGGGAGCGAGGTGCAGCCGACACCCACCTCCTCTGCACTGGAGGAAGAGGAAGA GGACCCAGATCTGGAGGATCTGAGCGGTGCTAAAGTGAATGCCCCCTACTACAGCGCGTGGGGCACGCTA GAGTATCACAACGCCATGGTTGTTGGCGCCGAAGAGGCGGAGGATGGTTCTGCGTGCGTTCGGGTACTCT ACCTATACCCTACCCACAAGTCCCTGAAGCCCTGCCCGTTCTTCCTGGAGGGAAAGTGCCGGTTCAAGGA GAACTGCAGGTTTTCCCATGGCCAGTTGGTATCTGTGGATGAGCTGCGTCCCTTCCAGGATCCAGACTTA AGCTTACTACAGACTGGCTCTGCATGTCTGGCCAAGCACCAGGATGGCCTTTGGCACCCAGCACGGATCA CTGATGTGGACAATGGCTACTATACTGTTAAGTTTGACTCGTTGCTGCTGAAAGAGGCTGTGGTGGAGGG GGACAGCATCCTGCCTCCTCTACGCACTGAGGCTACAGATTCATCTGACTCAGACACTGGTGATGCAAGT GACTCCAGCTATGCCAGAGTGGTGGAAGCAAACACAGTGGACACTGGGACCTGCAGTTCTGCCTTTGCTG GCTGGGAGGTACACACACGGGGCATTGGCTCCAAACTCCTCGTCAAGATGGGCTATGAGTTTGGCAAGGG TTTGGGCCGGCATGCAGAAGGCCGGGTGGAGCCCATCCATGCTGTAGTGTTGCCTAGAGGGAAATCACTG GACCAGTGCGCAGAGATACTGCAGAAGAAGACAAAGCAAGGCCAGACTGGTGCCAGCAGGCCTCCAAGAT GTCGGAGGAGGAGTAGTAGACCTGAGGGCCGCCCACCCCCTCGGAATGTGTTTGACTTTTTGAATGAAAA ACTGCAAAGCCAGGTTCCTGGGACCCCAGATGCTGGAGTGGATACCCCAGAGAGAAGGAACAAAGACATG TATCATGCCAGCAAGAGTGCCAAGCAGGCTTTGAGCCTGCAGCTCTTCCAGACTGAGGAGAAGATAGAGC GGACCCAGCGGGACATCCGGGGCATCCAGGAGGCCCTAACCCGCAACACTGGCCGGCATGGTATGGCGAC AGCCCATCTGCAGGAGAAGCTGGAGGGAGCCCAGCGGCAGCTGGGTCAGCTGCGTGCTCAGGAGGCCGAC CTACAGAGGAAACAGCGGAAGGCAGACACTCACAGGAAGATGACGGAGTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR203531 representing NM_001009656
Red=Cloning site Green=Tags(s) MDEDNLETALQTYRAQLQQVELALGAGLDASEQADLRQLQGDLKELIELTEASLLSVRKSKLLSTVDQEH QEDAEYLAFQKAIAEEAPVDPGNDSKTVPGSEVQPTPTSSALEEEEEDPDLEDLSGAKVNAPYYSAWGTL EYHNAMVVGAEEAEDGSACVRVLYLYPTHKSLKPCPFFLEGKCRFKENCRFSHGQLVSVDELRPFQDPDL SLLQTGSACLAKHQDGLWHPARITDVDNGYYTVKFDSLLLKEAVVEGDSILPPLRTEATDSSDSDTGDAS DSSYARVVEANTVDTGTCSSAFAGWEVHTRGIGSKLLVKMGYEFGKGLGRHAEGRVEPIHAVVLPRGKSL DQCAEILQKKTKQGQTGASRPPRCRRRSSRPEGRPPPRNVFDFLNEKLQSQVPGTPDAGVDTPERRNKDM YHASKSAKQALSLQLFQTEEKIERTQRDIRGIQEALTRNTGRHGMATAHLQEKLEGAQRQLGQLRAQEAD LQRKQRKADTHRKMTEF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001009656 |
| ORF Size | 1521 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001009656.1, NP_001009656.1 |
| RefSeq Size | 1930 bp |
| RefSeq ORF | 1524 bp |
| Locus ID | 296478 |
| UniProt ID | Q5PPF5 |
| MW | 56.2 kDa |
| Gene Summary | Transcription repressor that specifically binds the 5'-GGAG[GA]A[GA]A-3' consensus sequence. Represses transcription by recruiting the chromatin multiprotein complex NuRD to target promoters. Negatively regulates expression of EGFR, a gene involved in cell proliferation, survival and migration. Its ability to repress genes of the EGFR pathway suggest it may act as a tumor suppressor (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN203531 | Zgpat (untagged ORF) - Rat zinc finger, CCCH-type with G patch domain (Zgpat), (10 ug) |
CNY 6,560.00 |
|
| RR203531L3 | Lenti ORF clone of Zgpat (Myc-DDK-tagged ORF) - Rat zinc finger, CCCH-type with G patch domain (Zgpat), (10 ug) |
CNY 8,460.00 |
|
| RR203531L4 | Lenti ORF clone of Zgpat (mGFP-tagged ORF) - Rat zinc finger, CCCH-type with G patch domain (Zgpat), (10 ug) |
CNY 9,220.00 |
