Arcn1 (NM_001007662) Rat Tagged ORF Clone

CAT#: RR203391

  • TrueORF®

Arcn1 (Myc-DDK-tagged ORF) - Rat archain 1 (Arcn1), (10 ug)



  "NM_001007662" in other vectors (3)

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CNY 6,560.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Synonyms MGC94158
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR203391 representing NM_001007662
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGCTGTTGGCGGCAGCGGTCTGCACGAAAGCGGGAAAGGCAATTGTTTCTCGACAGTTTGTGGAGA
TGACCCGAACTCGGATTGAGGGGTTATTAGCAGCTTTCCCAAAACTCATGAACACTGGAAAACAACATAC
TTTTGTTGAAACAGAAAGTGTAAGATATGTCTACCAACCTATGGAGAAACTGTACATGGTATTGATCACT
ACAAAAAACAGCAATATCTTAGAAGATTTGGAGACTCTACGGCTCTTCTCAAGGGTGATCCCTGAATATT
GCCGAGCCTTAGAAGAAAATGAAATATCTGAGCACTGTTTTGATTTGATTTTTGCTTTTGATGAAATTGT
TGCCCTGGGATACCGGGAGAATGTTAACCTGGCACAGATCAGAACTTTCACAGAAATGGACTCTCATGAG
GAGAAAGTATTCAGAGCAGTCAGAGAGACTCAAGAACGTGAAGCTAAAGCTGAAATGCGGCGTAAAGCAA
AGGAATTACAACAGGCCCGGAGAGACGCGGAGAGACAGGGAAAGAAAGCACCAGGATTTGGGGGATTTGG
TAGTTCTGCAGTGTCTGGAGGCAGCACAGCAGCCATGATCACAGAGACTATCATTGAAACTGATAAACCA
AAAGTGGCACCCGCACCAGCCAGACCTTCAGGCCCCAGCAAGGCTTTGAAACTTGGAGCTAAAGGAAAGG
AAGTAGATAACTTTGTGGACAAACTGAAATCTGAAGGCGAAACTATTATGTCTTCTAACATGGGGAAGCG
CACCTCGGAAGCAGCCAAAGTGCATGCTCCACCCATTAATATGGAAAGTGTGCATATGAAGATTGAAGAA
AAGATCACACTGACCTGTGGGAGAGATGGAGGATTACAGAATATGGAGTTGCATGGCATGATCATGCTTA
GGATCTCAGATGACAAGTTTGGCCGGATTCGTCTTCATGTAGAAAATGAAGATAAGAAAGGGGTGCAGCT
GCAGACCCATCCAAATGTGGATAAAAAACTTTTTACTGCAGAGTCTCTCATTGGCTTGAAAAACCCAGAG
AAGTCATTTCCAGTCAACAGCGATGTAGGGGTGCTAAAGTGGAGACTACAAACAACAGAGGAATCTTTTA
TTCCTTTGACAATTAATTGCTGGCCTTCTGAAAGTGGAAACGGCTGTGATGTCAACATAGAATATGAACT
ACAGGAAGATAATTTAGAGCTCAATGATGTGGTCATCACCATCCCACTCCCATCGGGTGTTGGTGCACCT
GTGATCGGTGAGATCGATGGGGAATATCGACACGACAGTCGGAGAAATACCTTGGAGTGGTGCCTGCCAG
TGATTGATGCCAAAAATAAGAGTGGCAGCCTGGAGTTCAGCATTCCTGGGCAGCCCAATGACTTCTTCCC
TGTTCAAGTTTCTTTCATCTCCAAAAAGAATTACTGCAACATACAGGTTACCAAAGTGACCCAGGTAGAT
GGAAACAGCCCTGTTAGGTTTTCCACAGAGACCACTTTCTTAGTGGACAAATATGAAATCCTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR203391 representing NM_001007662
Red=Cloning site Green=Tags(s)

MVLLAAAVCTKAGKAIVSRQFVEMTRTRIEGLLAAFPKLMNTGKQHTFVETESVRYVYQPMEKLYMVLIT
TKNSNILEDLETLRLFSRVIPEYCRALEENEISEHCFDLIFAFDEIVALGYRENVNLAQIRTFTEMDSHE
EKVFRAVRETQEREAKAEMRRKAKELQQARRDAERQGKKAPGFGGFGSSAVSGGSTAAMITETIIETDKP
KVAPAPARPSGPSKALKLGAKGKEVDNFVDKLKSEGETIMSSNMGKRTSEAAKVHAPPINMESVHMKIEE
KITLTCGRDGGLQNMELHGMIMLRISDDKFGRIRLHVENEDKKGVQLQTHPNVDKKLFTAESLIGLKNPE
KSFPVNSDVGVLKWRLQTTEESFIPLTINCWPSESGNGCDVNIEYELQEDNLELNDVVITIPLPSGVGAP
VIGEIDGEYRHDSRRNTLEWCLPVIDAKNKSGSLEFSIPGQPNDFFPVQVSFISKKNYCNIQVTKVTQVD
GNSPVRFSTETTFLVDKYEIL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001007662
ORF Size 1533 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001007662.1, NP_001007663.1
RefSeq Size 3485 bp
RefSeq ORF 1536 bp
Locus ID 300674
UniProt ID Q66H80
MW 57.2 kDa
Gene Summary The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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