Mpzl1 (NM_001007728) Rat Tagged ORF Clone

CAT#: RR203090

  • TrueORF®

Mpzl1 (Myc-DDK-tagged ORF) - Rat myelin protein zero-like 1 (Mpzl1), (10 ug)



  "NM_001007728" in other vectors (3)

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CNY 3,990.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Synonyms MGC93692
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR203090 representing NM_001007728
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGAGGCCGTCGGAGCCGTGACATTGATTGCGGCCCCGGCCCGCCGGCGCTGGCTGTGGTCGGCGC
TAGCCGCGATGCTCGGGCTGTTGACAGCTAGAATATCAGCCTTGGAGGTACATACTCCTAAAGAAATCTT
TGTGGTGAATGGGACACAAGGGAAGCTCACGTGCACGTTTGACTCTCCTAATACAACTGGGTGGTTGACC
ACCGTCTCCTGGAGCTTCCAGCCAGAAGGCACCGACAGCGCCGTGTCGTTTTTCCACTACTCACAAGGAC
AAGTGTACATCGGGGATTATCCACCGTTTAAAGACCGAGTCACCTGGGCTGGGGACCTTGACAAGAAAGA
TGCATCGATCAATATAGAAAACATTCAGGCTGTTCACAATGGCACCTATATCTGCGATGTCAAAAACCCT
CCTGACATTGTTGTGCGGCCTGGGCAGATTAGGCTCCATGTGGTGGAAATAGACAACCTGCTGGTGTTCC
TGGTTTGGGTGGTGGTGGGCACTGTCACTGCTGTGGTCCTTGGGCTCACTCTACTCATCAGCTTGGTCCT
GGTCGTCCTCTACAGACGGAAGCATTCGAAGCGGGATTATACCGGCTGCAGTACCTCAGAGCGTTTGTCA
CCAGTTAAGCAGGCTCCACGGAAGTGTCCCTCCGACACAGAGGGTCTAGTAAAGAGTCCGCCTTCCGCCG
GATCTCACCAGGGCCCAGTCATATACGCACAGTTAGACCACTCTGGCGGACATCACAGTGGCAAGATTAA
TAAGTCAGAGTCTGTTGTGTATGCGGACATCCGGAAAGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR203090 representing NM_001007728
Red=Cloning site Green=Tags(s)

MAEAVGAVTLIAAPARRRWLWSALAAMLGLLTARISALEVHTPKEIFVVNGTQGKLTCTFDSPNTTGWLT
TVSWSFQPEGTDSAVSFFHYSQGQVYIGDYPPFKDRVTWAGDLDKKDASINIENIQAVHNGTYICDVKNP
PDIVVRPGQIRLHVVEIDNLLVFLVWVVVGTVTAVVLGLTLLISLVLVVLYRRKHSKRDYTGCSTSERLS
PVKQAPRKCPSDTEGLVKSPPSAGSHQGPVIYAQLDHSGGHHSGKINKSESVVYADIRKD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001007728
ORF Size 810 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001007728.1, NP_001007729.1
RefSeq Size 2291 bp
RefSeq ORF 813 bp
Locus ID 360871
UniProt ID Q6AYT8
MW 29.5 kDa
Gene Summary Cell surface receptor, which is involved in signal transduction processes. Recruits PTPN11/SHP-2 to the cell membrane and is a putative substrate of PTPN11/SHP-2. Is a major receptor for concanavalin-A (ConA) and is involved in cellular signaling induced by ConA, which probably includes Src family tyrosine-protein kinases. May be involved in regulation of integrin-mediated cell motility (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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