Wt1 (NM_031534) Rat Tagged Lenti ORF Clone
CAT#: RR203084L4
- LentiORF®
Lenti ORF clone of Wt1 (mGFP-tagged ORF) - Rat Wilms tumor 1 (Wt1), (10 ug)
"NM_031534" in other vectors (5)
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CNY 8,460.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | mGFP |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RR203084).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_031534 |
ORF Size | 1344 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_031534.2, NP_113722.2 |
RefSeq Size | 2645 bp |
RefSeq ORF | 1347 bp |
Locus ID | 24883 |
UniProt ID | P49952 |
Gene Summary | This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It plays an essential role in the normal development of the urogenital system, and the human gene is mutated in a small subset of patients with Wilm's tumors. Authors of PMID:7926762 provide evidence that Wt1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN203084 | Wt1 (untagged ORF) - Rat Wilms tumor 1 (Wt1), (10 ug) |
CNY 5,800.00 |
|
RR203084 | Wt1 (Myc-DDK-tagged ORF) - Rat Wilms tumor 1 (Wt1), (10 ug) |
CNY 4,024.00 |
|
RR203084L1 | Lenti ORF clone of Wt1 (Myc-DDK-tagged ORF) - Rat Wilms tumor 1 (Wt1), (10 ug) |
CNY 6,424.00 |
|
RR203084L2 | Lenti ORF clone of Wt1 (mGFP-tagged ORF) - Rat Wilms tumor 1 (Wt1), (10 ug) |
CNY 8,460.00 |
|
RR203084L3 | Lenti ORF clone of Wt1 (Myc-DDK-tagged ORF) - Rat Wilms tumor 1 (Wt1), (10 ug) |
CNY 7,600.00 |