A2m (NM_012488) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	A2m1; A2maa; A2MAC1; A2mb; Mam
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR203025 representing NM_012488 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAAGCACAGGCTCCGGAGCCTGGCCCTGCTGCCACTGCTGCTGCGGCTGCTGCTGCTGCTGCTGC CCACCGATGCCTCAGCTCCACAAAAACCAATCTACATGGTGATGGTTCCCTCCCTGCTCCACGCAGGCAC CCCCGAGAAGGCCTGCTTCCTGTTCAGCCATCTAAACGAGACAGTGGCTGTGAGAGTGTCCTTGGAGTCT GTCCGTGGGAACCAAAGCCTCTTCACTGACCTTGTAGTTGACAAGGACCTATTCCACTGTACCTCCTTCA CCGTCCCACAGTCTTCATCCGATGAGGTGATGTTTTTCACTGTCCAAGTAAAAGGAGCAACTCATGAGTT CAGGAGGCGGAGCACGGTGCTGGTTAAGAAGAAAGAGAGCCTGGTCTTTGCTCAGACTGACAAGCCCATC TACAAACCAGGACAGACAGTGAGATTTCGTGTTGTCTCATTGGACGAAAGTTTCCATCCCCTTAATGAAT TGATTCCTCTACTGTACATTCAGGATCCCAAAAACAATCGCATTGCACAATGGCAGAATTTCAATTTAGA GGGTGGCCTCAAACAGCTGTCCTTCCCCCTCTCCTCAGAGCCCACTCAGGGCTCCTACAAGGTGGTGATA CGTACAGAATCAGGCAGGACCGTCGAGCACCCTTTCTCTGTGGAGGAATTCGTGCTTCCCAAGTTCGAAG TGAGAGTGACAGTTCCAGAAACAATCACCATCCTGGAGGAAGAGATGAATGTGTCCGTGTGTGGAATATA CACCTATGGGAAGCCTGTTCCAGGACGTGTGACTGTAAACATTTGCAGAAAGTACAGTAATCCTTCTAAC TGCTTCGGCGAAGAGTCCGTGGCTTTCTGTGAGAAACTCAGCCAACAGTTAGACGGCCGTGGCTGCTTCT CACAGCTAGTGAAAACCAAGTCCTTCCAGCTAAAGAGACAAGAGTATGAGATGCAGCTCGATGTACATGC CAAGATCCAAGAAGAAGGAACAGGTGTGGAAGAAACTGGAAAGGGGCTCACTAAGATCACAAGAACCATA ACCAAACTATCATTTGTGAACGTGGATTCACATTTCAGACAAGGAATTCCTTTCGTTGGACAGGTGCTCC TGGTGGATGGGAGAGGCACCCCTATTCCGTATGAAACGATCTTCATTGGGGCGGATGAAGCAAACCTGTA CATAAATACAACCACTGATAAGCACGGCCTGGCGAGGTTCTCCATCAACACCGATGACATCATGGGCACG TCCCTAACTGTCAGGGCCAAATACAAGGATAGCAACGCCTGCTATGGATTCAGATGGTTGACAGAAGAGA ATGTAGAGGCTTGGCACACTGCCTACGCTGTTTTCTCACCAAGCAGAAGCTTCCTGCACCTGGAATCCCT GCCTGATAAACTGCGCTGTGACCAAACCCTGGAGGTCCAGGCACATTACATTCTAAATGGCGAGGCCATG CAGGAGCTGAAGGAGCTCGTCTTCTACTATCTGATGATGGCCAAGGGAGGCATCGTACGGGCGGGGACTC ACGTTCTGCCCCTGAAGCAGGGACAAATGAGAGGTCACTTTTCCATACTCATCTCGATGGAGACAGACCT GGCTCCCGTGGCTCGACTGGTCCTCTATGCCATCCTACCCAATGGAGAAGTGGTTGGAGACACTGCTAAA TATGAGATTGAGAACTGCCTGGCTAACAAGGTGGATTTGGTCTTCCGCCCGAATAGCGGTCTTCCAGCTA CCCGTGCCCTCCTTAGTGTCATGGCTTCTCCTCAGTCCCTTTGTGGCCTGCGAGCTGTGGACCAAAGCGT GCTGCTCATGAAACCTGAGACTGAGCTCTCCGCATCCCTGATTTATGACCTGCTACCAGTGAAAGACCTC ACTGGCTTCCCTCAGGGTGCGGATCAACGGGAAGAAGACACTAATGGCTGCGTTAAGCAAAATGACACTT ACATTAATGGAATCCTGTACTCGCCAGTGCAGAATACAAATGAAGAGGACATGTACGGCTTCCTAAAGGA TATGGGCTTAAAGGTATTTACCAACTCGAACATCCGTAAACCCAAAGTCTGCGAACGGCTCAGAGACAAT AAAGGAATACCAGCTGCGTACCACCTCGTAAGCCAAAGCCACATGGACGCTTTTCTAGAGTCTTCAGAGT CTCCCACAGAGACTAGGCGAAGCTACTTCCCTGAGACGTGGATCTGGGACTTGGTGGTGGTGGACTCAGC AGGAGTGGCTGAAGTGGAAGTGACAGTCCCTGACACCATCACTGAATGGAAGGCCGGGGCCTTCTGCCTG TCTAATGACACTGGTCTGGGCCTGTCTCCTGTGGTCCAATTCCAAGCCTTCCAGCCCTTCTTCGTGGAGC TCACAATGCCCTACTCCGTGATCCGTGGAGAAGCCTTCACGCTCAAGGCCACTGTGCTGAACTACCTCCC TACATGCATCCGGGTTGCCGTGCAGCTGGAGGCCTCTCCCGATTTTCTGGCTGCCCCAGAGGAGAAGGAA CAAAGGTCTCACTGCATCTGTATGAACCAGCGGCACACCGCGTCCTGGGCAGTGATCCCCAAGTCATTAG GAAATGTGAATTTCACAGTTAGTGCAGAGGCACTGAACTCTAAGGAGCTGTGTGGGAATGAGGTACCGGT GGTCCCTGAACAGGGCAAAAAAGACACGATCATCAAGTCCCTGCTGGTTGAACCCGAAGGTCTAGAGAAC GAAGTGACATTTAACAGTCTGCTTTGTCCAATGGGTGCTGAGGTATCTGAACTGATAGCCCTGAAGCTGC CATCAGACGTGGTAGAGGAATCTGCCAGAGCCTCTGTCACAGTTTTGGGAGATATATTGGGTTCTGCCAT GCAGAATACACAGGATCTCCTCAAGATGCCCTATGGCTGTGGAGAACAGAACATGGTTCTATTTGCTCCT AATATCTATGTCCTGGACTATCTGAATGAAACACAGCAGCTGACACAGGAGATCAAGACCAAGGCCATTG CCTATCTCAATACGGGCTACCAAAGACAATTAAACTACAAGCACCGGGATGGCTCCTACAGCACCTTTGG GGATAAACCTGGCAGGAATCATGCCAATACCTGGCTCACAGCCTTTGTACTGAAGAGTTTTGCTCAGGCT CGAAAATATATCTTCATCGATGAAGTACACATCACCCAAGCCCTCTTATGGCTCTCTCAGCAGCAGAAGG ACAATGGTTGTTTCAGGAGCTCCGGGTCACTGCTCAACAATGCCATGAAGGGAGGAGTAGAAGATGAAGT CACCTTGTCTGCCTACATCACCATAGCTCTCCTGGAGATGTCTCTTCCTGTCACTCATCCTGTTGTCCGC AATGCCCTCTTTTGCCTGGACACAGCCTGGAAGTCAGCAAGGGGAGGAGCTGGTGGCAGCCATGTCTACA CTAAGGCGCTGTTGGCCTATGCATTTGCCCTTGCTGGTAACCAGGACACGAAGAAGGAGATCCTGAAATC ACTCGATGAGGAGGCTGTAAAAGAAGAAGATTCTGTCCACTGGACCAGACCTCAGAAACCCAGCGTGTCA GTGGCCCTCTGGTACCAACCCCAGGCTCCCTCGGCTGAGGTAGAGATGACTGCATATGTGCTCCTGGCTT ATCTTACCACTGAGCCAGCTCCAACCCAAGAGGACCTAACGGCTGCCATGCTCATCGTGAAGTGGCTCAC AAAGCAGCAGAATTCCCACGGTGGCTTCTCTTCCACCCAGGACACTGTAGTGGCTCTCCACGCTTTGTCC AAATACGGGTCCGCCACTTTCACAAGAGCTAAGAAAGCTGCACAGGTGACCATCCATTCTTCGGGCACAT TTTCTACAAAATTCCAAGTCAACAACAACAACCAATTATTACTCCAGAGAGTCACATTGCCGACTGTGCC TGGGGATTACACCGTGAAGGTGACAGGAGAAGGCTGTGTCTATCTCCAGACATCCTTGAAATACAGTGTT CTCCCGAGAGAGGAGGAGTTCCCCTTCACTGTGGTGGTGCAGACTCTGCCTGGGACATGTGAGGATCCCA AAGCTCACACCAGCTTCCAGATCTCACTCAACATCAGTTACACTGGAAGCCGTTCTGAATCCAACATGGC AATTGCTGACGTGAAGATGGTGTCCGGCTTCATCCCCTTGAAACCAACAGTGAAAATGCTTGAAAGATCT GTGCATGTGAGCCGAACAGAAGTCAGCAATAACCATGTCTTGATTTACCTGGATAAGGTGTCAAATCAGA CGGTGAACTTGTCCTTCACGGTTCAGCAAGATATTCCAATAAGAGACCTGAAGCCAGCCGTAGTGAAAGT CTACGATTACTATGAGAAAGATGAGTTTGCAGTTGCAAAATACAGCGCTCCCTGCAGCACAGATTATGGA AATGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR203025 representing NM_012488 Red=Cloning site Green=Tags(s) MGKHRLRSLALLPLLLRLLLLLLPTDASAPQKPIYMVMVPSLLHAGTPEKACFLFSHLNETVAVRVSLES VRGNQSLFTDLVVDKDLFHCTSFTVPQSSSDEVMFFTVQVKGATHEFRRRSTVLVKKKESLVFAQTDKPI YKPGQTVRFRVVSLDESFHPLNELIPLLYIQDPKNNRIAQWQNFNLEGGLKQLSFPLSSEPTQGSYKVVI RTESGRTVEHPFSVEEFVLPKFEVRVTVPETITILEEEMNVSVCGIYTYGKPVPGRVTVNICRKYSNPSN CFGEESVAFCEKLSQQLDGRGCFSQLVKTKSFQLKRQEYEMQLDVHAKIQEEGTGVEETGKGLTKITRTI TKLSFVNVDSHFRQGIPFVGQVLLVDGRGTPIPYETIFIGADEANLYINTTTDKHGLARFSINTDDIMGT SLTVRAKYKDSNACYGFRWLTEENVEAWHTAYAVFSPSRSFLHLESLPDKLRCDQTLEVQAHYILNGEAM QELKELVFYYLMMAKGGIVRAGTHVLPLKQGQMRGHFSILISMETDLAPVARLVLYAILPNGEVVGDTAK YEIENCLANKVDLVFRPNSGLPATRALLSVMASPQSLCGLRAVDQSVLLMKPETELSASLIYDLLPVKDL TGFPQGADQREEDTNGCVKQNDTYINGILYSPVQNTNEEDMYGFLKDMGLKVFTNSNIRKPKVCERLRDN KGIPAAYHLVSQSHMDAFLESSESPTETRRSYFPETWIWDLVVVDSAGVAEVEVTVPDTITEWKAGAFCL SNDTGLGLSPVVQFQAFQPFFVELTMPYSVIRGEAFTLKATVLNYLPTCIRVAVQLEASPDFLAAPEEKE QRSHCICMNQRHTASWAVIPKSLGNVNFTVSAEALNSKELCGNEVPVVPEQGKKDTIIKSLLVEPEGLEN EVTFNSLLCPMGAEVSELIALKLPSDVVEESARASVTVLGDILGSAMQNTQDLLKMPYGCGEQNMVLFAP NIYVLDYLNETQQLTQEIKTKAIAYLNTGYQRQLNYKHRDGSYSTFGDKPGRNHANTWLTAFVLKSFAQA RKYIFIDEVHITQALLWLSQQQKDNGCFRSSGSLLNNAMKGGVEDEVTLSAYITIALLEMSLPVTHPVVR NALFCLDTAWKSARGGAGGSHVYTKALLAYAFALAGNQDTKKEILKSLDEEAVKEEDSVHWTRPQKPSVS VALWYQPQAPSAEVEMTAYVLLAYLTTEPAPTQEDLTAAMLIVKWLTKQQNSHGGFSSTQDTVVALHALS KYGSATFTRAKKAAQVTIHSSGTFSTKFQVNNNNQLLLQRVTLPTVPGDYTVKVTGEGCVYLQTSLKYSV LPREEEFPFTVVVQTLPGTCEDPKAHTSFQISLNISYTGSRSESNMAIADVKMVSGFIPLKPTVKMLERS VHVSRTEVSNNHVLIYLDKVSNQTVNLSFTVQQDIPIRDLKPAVVKVYDYYEKDEFAVAKYSAPCSTDYG NA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_012488
ORF Size	4416 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_012488.2, NP_036620.2
RefSeq Size	4614 bp
RefSeq ORF	4419 bp
Locus ID	24153
UniProt ID	P06238
MW	163.8 kDa
Gene Summary	plays a role in acute phase response; activated form induces a decrease in N-methyl-D-aspartate (NMDA) mediated calcium signaling [RGD, Feb 2006]

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