Grin2d (NM_022797) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	GluN2D
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR203008 representing NM_022797 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCGGCGCCGGTGGCCCCCGCGGCCCTCGGGGCCCCGCTAAGATGCTGCTGCTGCTGGCGCTGGCGT GCGCCAGCCCGTTCCCGGAGGAGGTGCCGGGGCCGGGCGCGGTCGGCGGGGGCACGGGCGGGGCGCGGCC ACTCAACGTGGCGCTTGTCTTCTCCGGCCCGGCATATGCGGCAGAGGCGGCGCGCTTGGGCCCGGCCGTG GCAGCGGCAGTGCGCAGCCCCGGCCTGGACGTACGGCCCGTGGCGCTGGTGCTCAACGGCTCCGACCCTC CGAGCCTTGTGCTGCAGCTCTGCGACCTGCTGTCGGGGCTGCGCGTGCACGGCGTGGTGTTCGAGGACGA CTCGCGCGCGCCCGCCGTCGCGCCCATTCTCGACTTCCTGTCCGCGCAGACCTCGCTGCCCATCGTAGCC GTGCACGGCGGCGCCGCGCTCGTGCTCACACCCAAGGAGAAGGGCTCCACCTTCCTGCAGCTTGGCTCCT CCACAGAGCAACAGTTGCAGGTCATTTTTGAGGTGCTGGAGGAGTACGACTGGACATCCTTTGTGGCAGT GACCACGCGTGCCCCAGGCCATCGGGCCTTCTTGTCATACATCGAGGTGCTGACTGATGGCAGCCTGGTG GGCTGGGAGCATCGAGGAGCGCTGACACTGGACCCCGGGGCGGGTGAGGCTGTACTGGGCGCACAGCTCC GTAGTGTCAGTGCGCAGATCCGCCTGCTCTTCTGCGCCCGCGAGGAGGCAGAGCCTGTTTTCCGGGCAGC GGAAGAGGCCGGCCTCACTGGGCCTGGCTACGTCTGGTTCATGGTGGGACCCCAGCTGGCTGGAGGCGGG GGCTCCGGGGTCCCTGGGGAGCCCCTTCTTCTGCCAGGAGGTTCCCCACTGCCTGCCGGGCTGTTTGCAG TGGCCTCTGCTGGCTGGCGTGACGACTTGGCACGTCGAGTGGCTGCTGGTGTGGCGGTGGTGGCCAGAGG TGCCCAGGCCCTGCTGCGAGACTATGGCTTCCTGCCTGAGCTGGGTCATGACTGCCGCACCCAGAATCGC ACCCACCGCGGGGAGAGTCTGCACAGGTACTTCATGAACATCACCTGGGATAACCGAGACTACTCCTTCA ATGAGGATGGCTTTCTGGTGAACCCTTCACTGGTGGTCATCTCCCTCACCAGAGACAGGACATGGGAAGT GGTGGGCAGCTGGGAACAGCAGACCCTCCGCCTCAAGTACCCTCTATGGTCCCGCTATGGCCGCTTCCTG CAGCCAGTGGATGACACACAGCACCTCACTGTGGCCACGCTGGAGGAGAGACCCTTTGTCATTGTAGAGC CTGCAGACCCCATCAGCGGCACTTGCATCAGAGACTCGGTCCCCTGCCGGAGCCAGCTCAACCGTACCCA CAGCCCTCCGCCTGACGCTCCCCGCCCGGAGAAGAGATGCTGCAAGGGTTTCTGCATTGATATTCTGAAG AGGCTGGCGCACACCATCGGTTTCAGCTATGACCTCTACCTGGTTACCAACGGCAAGCATGGCAAGAAGA TCGATGGCGTCTGGAATGGCATGATTGGTGAGGTGTTCTATCAGCGTGCGGACATGGCCATCGGCTCTCT CACCATCAATGAGGAGCGGTCAGAGATCGTGGACTTCTCCGTCCCCTTTGTAGAGACAGGCATCAGCGTC ATGGTGGCACGCAGCAATGGCACTGTGTCCCCCTCTGCCTTCCTCGAGCCCTACAGCCCTGCTGTCTGGG TGATGATGTTCGTCATGTGCCTCACCGTGGTCGCCGTCACAGTTTTCATCTTTGAGTACCTCAGTCCTGT GGGCTATAACCGAAGCCTGGCCACGGGCAAACGCCCTGGTGGCTCTACCTTCACCATTGGGAAATCCATC TGGCTGCTGTGGGCCCTGGTCTTCAACAACTCCGTGCCAGTGGAGAATCCTCGGGGCACCACCAGCAAGA TCATGGTGCTGGTGTGGGCCTTCTTCGCGGTCATCTTTCTTGCCAGCTACACAGCCAATCTGGCCGCCTT CATGATCCAGGAGGAGTACGTGGACACCGTGTCTGGGCTCAGCGACCGGAAGTTCCAGCGACCCCAGGAG CAATACCCACCTCTGAAGTTTGGCACGGTGCCCAATGGGTCCACGGAAAAGAACATCCGGAGCAACTACC CTGACATGCACAGCTACATGGTGCGATACAACCAGCCGAGAGTGGAGGAGGCACTCACACAGCTCAAGGC AGGGAAACTGGACGCCTTCATCTATGACGCAGCAGTGCTCAACTACATGGCCCGCAAGGATGAGGGCTGC AAGCTGGTCACCATCGGCTCCGGCAAGGTCTTCGCCACCACTGGTTATGGCATCGCCCTACACAAGGGCT CCCGCTGGAAGAGGCCCATCGACCTGGCGCTGCTGCAGTTCCTGGGGGACGATGAGATTGAGATGCTGGA GCGGCTGTGGCTTTCAGGGATCTGCCACAATGACAAAATCGAAGTGATGAGTAGCAAGCTGGATATCGAC AACATGGCAGGTGTCTTCTACATGCTCCTTGTGGCCATGGGCCTCTCCTTGCTGGTCTTCGCCTGGGAAC ATCTGGTGTACTGGCGCCTGCGGCATTGCCTGGGTCCCACCCACCGCATGGATTTCCTACTGGCCTTCTC CAGGGGTATGTACAGCTGCTGCAGCGCCGAGGCTGCTCCACCGCCGGCCAAGCCTCCGCCACCGCCTCAG CCGCTGCCCAGTCCCGCATATCCCGCCGCTCGCCCGCCCCCTGGCCCCGCACCCTTCGTGCCCCGAGAGC GCGCAGCTGCCGACCGCTGGCGCCGAGCCAAGGGCACAGGGCCCCCGGGGGGCGCAGCGATAGCCGACGG CTTCCACCGATACTACGGCCCCATCGAGCCACAGGGGCTGGGCCTCGGCGAGGCACGCGCGGCACCGAGA GGCGCAGCCGGACGCCCACTGTCCCCACCCACCACACAGCCCCCGCAGAAGCCACCACCTTCCTACTTCG CCATCGTGCGCGAGCAGGAGCCGACCGAGCCCCCGGCCGGCGCCTTCCCCGGCTTCCCATCTCCGCCTGC GCCGCCCGCTGCGGCTGCCGCTGCCGTCGGGCCGCCACTGTGTCGCCTGGCTTTCGAGGACGAAAGCCCG CCCGCACCCTCGCGCTGGCCGCGTTCAGATCCCGAGAGCCAGCCTCTGTTGGGTGGGGGCGCGGGCGGCC CGAGCGCGGGGGCCCCAACCGCACCACCGCCGCGCCGTGCCGCGCCACCACCGTGCGCCTACCTGGACCT CGAGCCTTCGCCTTCGGACTCCGAGGACTCGGAGAGCCTGGGCGGAGCATCGCTCGGTGGCCTGGAGCCC TGGTGGTTCGCCGACTTCCCCTACCCGTATGCCGAGCGCCTCGGGCCGCCGCCCGGCCGCTACTGGTCGG TCGACAAGCTCGGGGGCTGGCGCGCTGGTAGCTGGGACTACCTGCCCCCGCGCGGAGGCCCCGCGTGGCA CTGCCGCCACTGCGCCAGCCTGGAGCTGCTACCGCCACCGCGCCACCTCAGCTGCTCGCACGACGGCTTA GACGGCGGCTGGTGGGCGCCTCCGCCTCCACCCTGGGCCGCGGGGCCACCTCCCCGGCGCCGGGCGCGCT GTGGTTGTCCGCGACCTCACCCACACCGCCCCCGGGCCTCGCACCGTGCGCCCGCCGCCGCGCCGCACCA CCACCGACACAGGCGCGCGGCCGGTGGCTGGGACTTCCCGCCGCCCGCGCCCACCTCGCGTTCGCTGGAG GACCTGAGCTCCTGCCCGCGGGCGGCCCCCACGCGCAGGCTCACCGGGCCATCCCGCCACGCGCGCCGCT GCCCGCACGCTGCGCACTGGGGGCCGCCCCTGCCCACCGCATCGCACCGGAGACACCGGGGCGGGGACCT GGGCACACGCAGGGGCTCTGCGCATTTCTCCAGCCTGGAGTCCGAGGTA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR203008 representing NM_022797 Red=Cloning site Green=Tags(s) MRGAGGPRGPRGPAKMLLLLALACASPFPEEVPGPGAVGGGTGGARPLNVALVFSGPAYAAEAARLGPAV AAAVRSPGLDVRPVALVLNGSDPPSLVLQLCDLLSGLRVHGVVFEDDSRAPAVAPILDFLSAQTSLPIVA VHGGAALVLTPKEKGSTFLQLGSSTEQQLQVIFEVLEEYDWTSFVAVTTRAPGHRAFLSYIEVLTDGSLV GWEHRGALTLDPGAGEAVLGAQLRSVSAQIRLLFCAREEAEPVFRAAEEAGLTGPGYVWFMVGPQLAGGG GSGVPGEPLLLPGGSPLPAGLFAVASAGWRDDLARRVAAGVAVVARGAQALLRDYGFLPELGHDCRTQNR THRGESLHRYFMNITWDNRDYSFNEDGFLVNPSLVVISLTRDRTWEVVGSWEQQTLRLKYPLWSRYGRFL QPVDDTQHLTVATLEERPFVIVEPADPISGTCIRDSVPCRSQLNRTHSPPPDAPRPEKRCCKGFCIDILK RLAHTIGFSYDLYLVTNGKHGKKIDGVWNGMIGEVFYQRADMAIGSLTINEERSEIVDFSVPFVETGISV MVARSNGTVSPSAFLEPYSPAVWVMMFVMCLTVVAVTVFIFEYLSPVGYNRSLATGKRPGGSTFTIGKSI WLLWALVFNNSVPVENPRGTTSKIMVLVWAFFAVIFLASYTANLAAFMIQEEYVDTVSGLSDRKFQRPQE QYPPLKFGTVPNGSTEKNIRSNYPDMHSYMVRYNQPRVEEALTQLKAGKLDAFIYDAAVLNYMARKDEGC KLVTIGSGKVFATTGYGIALHKGSRWKRPIDLALLQFLGDDEIEMLERLWLSGICHNDKIEVMSSKLDID NMAGVFYMLLVAMGLSLLVFAWEHLVYWRLRHCLGPTHRMDFLLAFSRGMYSCCSAEAAPPPAKPPPPPQ PLPSPAYPAARPPPGPAPFVPRERAAADRWRRAKGTGPPGGAAIADGFHRYYGPIEPQGLGLGEARAAPR GAAGRPLSPPTTQPPQKPPPSYFAIVREQEPTEPPAGAFPGFPSPPAPPAAAAAAVGPPLCRLAFEDESP PAPSRWPRSDPESQPLLGGGAGGPSAGAPTAPPPRRAAPPPCAYLDLEPSPSDSEDSESLGGASLGGLEP WWFADFPYPYAERLGPPPGRYWSVDKLGGWRAGSWDYLPPRGGPAWHCRHCASLELLPPPRHLSCSHDGL DGGWWAPPPPPWAAGPPPRRRARCGCPRPHPHRPRASHRAPAAAPHHHRHRRAAGGWDFPPPAPTSRSLE DLSSCPRAAPTRRLTGPSRHARRCPHAAHWGPPLPTASHRRHRGGDLGTRRGSAHFSSLESEV SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_022797
ORF Size	3969 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_022797.1, NP_073634.1
RefSeq Size	5246 bp
RefSeq ORF	3972 bp
Locus ID	24412
UniProt ID	Q62645
MW	143 kDa
Gene Summary	N-methyl-D-aspartate receptor subunit; expressed in mid-brain structures with peak expression at day P7 [RGD, Feb 2006]

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