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Eef2 (NM_017245) Rat Tagged ORF Clone
CAT＃: RR202878
- TrueORF®
Eef2 (Myc-DDK-tagged ORF) - Rat eukaryotic translation elongation factor 2 (Eef2), (10 ug)
"NM_017245" in other vectors (3)
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CNY 10,930.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Ef-2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR202878 representing NM_017245
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGAACTTCACAGTAGATCAGATCCGTGCCATCATGGACAAGAAAGCCAACATCCGGAACATGTCAG TCATCGCTCACGTGGACCACGGCAAGTCCACGTTGACCGACTCCCTTGTGTGCAAGGCTGGCATCATCGC CTCTGCCCGAGCCGGGGAGACACGCTTCACTGACACTCGAAAGGATGAGCAGGAGCGCTGCATCACCATC AAGTCCACTGCCATCTCCCTCTTCTATGAGCTCTCCGAGAACGACCTGAACTTCATTAAGCAGAGCAAGG ATGGCTCGGGCTTCCTCATCAACCTCATTGACTCTCCAGGTCATGTGGACTTCTCCTCAGAGGTGACCGC TGCTCTGCGTGTCACTGATGGAGCACTGGTGGTGGTGGACTGTGTGTCTGGTGTCTGTGTGCAGACAGAG ACAGTGCTGCGGCAGGCCATCGCTGAGCGCATCAAGCCCGTGCTGATGATGAACAAGATGGACCGGGCCC TGCTGGAACTGCAACTGGAGCCTGAGGAGCTCTACCAGACCTTCCAGCGCATCGTGGAGAACGTCAATGT CATCATCTCCACCTATGGCGAGGGCGAGAGTGGACCCATGGGCAATATCATGATCGATCCTGTCCTGGGC ACTGTAGGCTTTGGCTCTGGCCTGCATGGGTGGGCCTTCACACTGAAGCAGTTTGCAGAGATGTATGTGG CCAAGTTTGCAGCCAAGGGTGAGGGCCAGCTGGGTGCAGCTGAGCGGGCCAAGAAGGTAGAAGACATGAT GAAGAAGCTGTGGGGAGACCGGTACTTTGACCCGGCCAACGGCAAGTTCAGCAAGTCAGCCAATAGCCCA GACGGGAAGAAACTTCCCCGCACCTTCTGCCAGCTCATCTTGGACCCCATCTTCAAGGTGTTTGATGCCA TCATGAACTTCAGGAAGGAGGAGACAGCCAAGCTGATCGAGAAGCTGGACATCAAGCTGGACAGTGAGGA CAAGGACAAGGAGGGCAAACCACTGCTGAAGGCTGTGATGCGTCGCTGGCTGCCTGCTGGTGACGCCCTG CTACAGATGATCACCATCCACTTGCCGTCCCCGGTCACTGCACAGAAGTACCGTTGTGAGCTGCTGTACG AGGGCCCACCTGATGACGAGGCCGCCATGGGTATTAAGAGCTGCGACCCCAAAGGCCCCCTAATGATGTA CATCTCCAAGATGGTGCCAACATCTGACAAAGGCCGCTTCTATGCCTTCGGTAGAGTGTTCTCTGGGGTG GTGTCCACAGGTCTGAAGGTCCGGATCATGGGCCCCAACTATACACCTGGGAAGAAGGAGGACCTGTACC TGAAGCCTATCCAGAGGACCATTCTGATGATGGGCCGCTATGTGGAACCCATTGAGGATGTGCCCTGTGG CAACATTGTGGGGTTGGTCGGGGTGGACCAGTTCCTTGTGAAGACCGGCACCATCACTACCTTTGAGCAC GCTCACAACATGCGGGTGATGAAGTTCAGCGTCAGCCCTGTCGTCAGGGTGGCAGTGGAGGCCAAGAACC CAGCTGACCTGCCCAAGCTGGTGGAGGGGCTGAAGCGGCTGGCTAAGTCTGACCCTATGGTGCAGTGCAT CATCGAGGAGTCTGGGGAGCACATCATTGCTGGTGCTGGAGAGCTGCACCTGGAGATCTGCCTTAAGGAC CTGGAGGAGGACCATGCCTGCATCCCCATCAAGAAATCTGACCCTGTCGTGTCCTACCGGGAGACAGTCA GTGAGGAGTCCAACGTGCTCTGTCTTTCCAAGTCCCCAAACAAGCACAACAGGCTGTACATGAAGGCCAG GCCCTTCCCTGATGGCCTAGCAGAGGACATCGATAAGGGTGAGGTATCTGCCCGCCAGGAGCTCAAGGCG CGTGCCCGTTACCTGGCCGAAAAGTATGAGTGGGATGTTGCTGAAGCCCGCAAGATCTGGTGCTTTGGAC CTGATGGCACTGGTCCCAACATTCTCACCGACATCACCAAGGGTGTGCAGTACCTGAATGAGATCAAGGA CAGTGTGGTGGCTGGATTCCAGTGGGCCACTAAGGAGGGTGCTCTTTGTGAGGAGAACATGCGTGGTGTG CGATTTGATGTCCATGATGTGACCCTGCATGCTGATGCCATCCACCGAGGAGGTGGTCAGATCATCCCCA CAGCGCGCCGCTGCCTCTATGCCAGTGTGCTGACTGCACAGCCCCGCCTCATGGAGCCTATCTACCTGGT GGAGATTCAGTGTCCTGAGCAAGTGGTGGGCGGAATCTATGGTGTCCTGAACAGGAAGCGTGGCCATGTC TTTGAGGAGTCCCAGGTAGCTGGGACCCCCATGTTCGTGGTCAAGGCCTACCTGCCTGTCAATGAATCCT TTGGCTTCACTGCCGACCTTCGATCCAACACTGGTGGCCAGGCCTTCCCCCAGTGTGTGTTTGACCACTG GCAGATCCTGCCTGGGGACCCCTTTGACAACAGCAGCCGCCCCAGCCAAGTTGTAGCTGAGACCCGCAAG CGCAAAGGCCTGAAGGAGGGCATCCCAGCGCTGGACAACTTCCTGGACAAACTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR202878 representing NM_017245
Red=Cloning site Green=Tags(s) MVNFTVDQIRAIMDKKANIRNMSVIAHVDHGKSTLTDSLVCKAGIIASARAGETRFTDTRKDEQERCITI KSTAISLFYELSENDLNFIKQSKDGSGFLINLIDSPGHVDFSSEVTAALRVTDGALVVVDCVSGVCVQTE TVLRQAIAERIKPVLMMNKMDRALLELQLEPEELYQTFQRIVENVNVIISTYGEGESGPMGNIMIDPVLG TVGFGSGLHGWAFTLKQFAEMYVAKFAAKGEGQLGAAERAKKVEDMMKKLWGDRYFDPANGKFSKSANSP DGKKLPRTFCQLILDPIFKVFDAIMNFRKEETAKLIEKLDIKLDSEDKDKEGKPLLKAVMRRWLPAGDAL LQMITIHLPSPVTAQKYRCELLYEGPPDDEAAMGIKSCDPKGPLMMYISKMVPTSDKGRFYAFGRVFSGV VSTGLKVRIMGPNYTPGKKEDLYLKPIQRTILMMGRYVEPIEDVPCGNIVGLVGVDQFLVKTGTITTFEH AHNMRVMKFSVSPVVRVAVEAKNPADLPKLVEGLKRLAKSDPMVQCIIEESGEHIIAGAGELHLEICLKD LEEDHACIPIKKSDPVVSYRETVSEESNVLCLSKSPNKHNRLYMKARPFPDGLAEDIDKGEVSARQELKA RARYLAEKYEWDVAEARKIWCFGPDGTGPNILTDITKGVQYLNEIKDSVVAGFQWATKEGALCEENMRGV RFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHV FEESQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRK RKGLKEGIPALDNFLDKL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017245 |
| ORF Size | 2574 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017245.2, NP_058941.1 |
| RefSeq Size | 2944 bp |
| RefSeq ORF | 2577 bp |
| Locus ID | 29565 |
| UniProt ID | P05197 |
| MW | 95.3 kDa |
| Gene Summary | catalyzes the translocation of peptidyl-tRNA from the ribosomal A site to the P site in translational elongation [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN202878 | Eef2 (untagged ORF) - Rat eukaryotic translation elongation factor 2 (Eef2), (10 ug) |
CNY 10,930.00 |
|
| RR202878L3 | Lenti ORF clone of Eef2 (Myc-DDK-tagged ORF) - Rat eukaryotic translation elongation factor 2 (Eef2), (10 ug) |
CNY 12,830.00 |
|
| RR202878L4 | Lenti ORF clone of Eef2 (mGFP-tagged ORF) - Rat eukaryotic translation elongation factor 2 (Eef2), (10 ug) |
CNY 13,970.00 |
