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Myocd (NM_182667) Rat Tagged ORF Clone
CAT＃: RR202749
- TrueORF®
Myocd (Myc-DDK-tagged ORF) - Rat transcription factor myocardin (Myocd), (10 ug)
"NM_182667" in other vectors (3)
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CNY 12,070.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Mycd |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR202749 representing NM_182667
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACACTCCTGGGGTCTGAACACTCTTTGCTGATTAGAAGGAAGTTCCGATCAGTCTTACAGTTACGGC TTCAACAGAGAAGGACCCAGGAGCAGCTGGCTAACCAAGGCTTAATACCACCCCTGAAAAGTCCAACTGA GTTCCATGACCCGAGGAAAAAACTGGATAGTGCCAAGACTGAAGACTCTCTGAGGCGCAAGGTCAGAAAC AGATCGGACCGTGCCAGCCTGGTTAATATGCACATTCTCCAAGCCTCCACGGCAGAAAGATCCATTCCAA CTGCTCAGATGAAGCTGAAGAGAGCCCGCCTTGCAGATGACCTCAACGAGAAGATTGCTCTCCGCCCAGG GCCCTTGGAACTGGTGGAGAAGAACATTCTGCCTATGGATTCTTCCGTGAAAGAGGCTATAAAAGGTACT GAGGTGAGCCTCTCCAAAGCAGCAGATGCATTTGCCTTTGAAGATGACAGCAGCAGGGATGGGCTTTCCC CAGATCAGGCTCGGAGCGAGGATCCCCAGGGCTCTGGAGGATCAACCCCAGACATCAAATCCACTGAGGC TCCTCTGGCTGGCCCTCTGGACACAATCCAGGATCTCACTCCTGGCTCAGAAAGTGACAAGAACGATACA GCCTCCCAGCTGAGCAACCAGTCAGACTCTGGGAAGCAGGTGCTTGGCCCTCTCAGCACCCCGATCCCTG TGCACACTGCGGTAAAGTCCAAGTCTTTGGGTGATAGTAAGAACCGCCACAAAAAGCCCAAAGACCCCAA ACCAAAGGTGAAGAAGCTCAAATACCACCAGTACATCCCCCCGGACCAGAAGGCAGAGAAATCCCCTCCC CCCATGGACTCTGCCTATGCTCGGCTGCTTCAGCAACAGCAGCTATTCCTGCAGCTACAGATCCTCAGCC AGCAGCAACAGCAGCAGCAACAGCAGCAACAGCAGCAGCAGCAACAGCAGCAACAGCAACGGTTCAGCTA CCCTGGGATGCACCAAGCACACCTCAAAGAGCCAAATGAACAGATGACCAGAAATCCGAATTCTTCCTCA ACACCACTGAACAATACCCCGCTGTCCCCTGTCAAAAATAGCCTTTCTGGACAAACTGGTGTTTCTTCTC TCAAACCAGGCCCCCTTCCACCAAACCTGGATGATCTCAAGGTGTCAGAGTTAAGACAACAGCTTCGAAT CCGGGGCTTGCCAGTGTCGGGCACCAAGACAGCACTGGTGGACCGGCTTCGACCCTTCCAGGATTGTGCT GGCAACCCTGTGCCCAACTTTGGGGACATCACAACTGTCACCTTTCCTGTCACGCCCAACACCTTGCCCA GTTATCAGTCCTCCCCATCAGGCTTCTACCACTTTGGCAGCACAAGCTCCAGCCCGCCCATCTCCCCCGC CTCATCTGACTTGTCTGCTGCAGGGTCCCTGCCAGACACCTTCACCGATGCGTCACCTGGCTTCGGCCTG CATGCATCGCCGGTGCCCGCCTGTACGGATGAGAGTCTGCTGAGCAGCCTGAATGGGGGCTCGGGCCCCT CCGAGCCTGACGGGCTAGACTCTGAGAAGGACAAGATGCTGGTAGAGAAGCAAAAGGTGATCAACCAGCT CACCTGGAAGCTGCGGCAAGAGCAGCGGCAGGTGGAGGAGCTGAGAATGCAACTGCAGAAGCAGAAGAGC GGCTGCAATGACCAGAAGCCACTGCCCTTCTTGGCCACCACCATCAAGCAGGAAGATGTCTCTAGCTGTC CCTTCGCAGCCCAGCAGGCATCTGGGAAGGGACAGGGCCACAGCTCCGACAGTCCCCCTCCGGCCTGTGA GACTGCTCAGCTGCTGCCTCACTGTGTGGAGTCCTCAGGTCAAACCCATGTACTCTCCTCCACATTTCTC AGCCCCCAGTGCTCCCCTCAGCACTCCCCCCTGGGGACCCTGAAGAGCCCGCAACACATCAGCCTGCCCC CATCGCCTAACAACCATTACTTCCTGGCTTCCTCCTCTGGGGCTCAGAGAGAGAACCATGGGGTGTCTTC ACCCAACAGCAGCCAAGGGTGTGCACAGATGACTGGTTTACAATCTTCTGACAAGGTGGGGCCAACGTTT TCAATTCCATCCCCAACTTTTCCTAAGTCGAGTCCAACAGTTCCGGAGATAACCCAGCCCCCATCCTACG AAGATGCAGTGAAACAGCAAATGACTCGGAGTCAGCAGATGGATGAACTCCTGGATGTCCTCATTGAAAG TGGAGAAATGCCAGCGGATGCCAGGGAAGATCATTCATGTCTTCAAAAAATCCCAAAGATACCTGGGTCC TCCTGCAGCCCAACCACCATCCTCCCCAAGTCCTCGGCTTCCTTTGAACAGGCATCTTCGGGAGGCCAGA TCTCCTTCGATCACTATGCCACCGACAGTGAGGAACACCTGGAAGTCTTATTGAATTCTCACAGCCCCAT TGGGAAGGTGAGCGATGTTACCCTCCTCAAAATCGGAAGCGAGGAGCCTCCTTTTGATGGGATCATGGAT GGCTTCCCAGGGAAGGCTGCGGAAGATCTCTTCAGTGCTCATGAGCTCTTGCCTGGGCCGCTCTCCCCTA TGCATACACAGTTGTCACCTCCTTCTGTGGACAGCAGTGGTCTGCAGCTGAGCTTCACGGAATCTCCTTG GGAAACAATGGAATGGCTGGACCTCACTCCACCTAGTTCCACACCAGGCTTCAGCAACCTCACCTCCAGC GGGCCCAGCATTTTCAACATCGATTTTCTGGATGTCACGGATCTCAATTTGAATTCCCCTATGGACCTCC ACTTACAGCAGTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR202749 representing NM_182667
Red=Cloning site Green=Tags(s) MTLLGSEHSLLIRRKFRSVLQLRLQQRRTQEQLANQGLIPPLKSPTEFHDPRKKLDSAKTEDSLRRKVRN RSDRASLVNMHILQASTAERSIPTAQMKLKRARLADDLNEKIALRPGPLELVEKNILPMDSSVKEAIKGT EVSLSKAADAFAFEDDSSRDGLSPDQARSEDPQGSGGSTPDIKSTEAPLAGPLDTIQDLTPGSESDKNDT ASQLSNQSDSGKQVLGPLSTPIPVHTAVKSKSLGDSKNRHKKPKDPKPKVKKLKYHQYIPPDQKAEKSPP PMDSAYARLLQQQQLFLQLQILSQQQQQQQQQQQQQQQQQQQQRFSYPGMHQAHLKEPNEQMTRNPNSSS TPLNNTPLSPVKNSLSGQTGVSSLKPGPLPPNLDDLKVSELRQQLRIRGLPVSGTKTALVDRLRPFQDCA GNPVPNFGDITTVTFPVTPNTLPSYQSSPSGFYHFGSTSSSPPISPASSDLSAAGSLPDTFTDASPGFGL HASPVPACTDESLLSSLNGGSGPSEPDGLDSEKDKMLVEKQKVINQLTWKLRQEQRQVEELRMQLQKQKS GCNDQKPLPFLATTIKQEDVSSCPFAAQQASGKGQGHSSDSPPPACETAQLLPHCVESSGQTHVLSSTFL SPQCSPQHSPLGTLKSPQHISLPPSPNNHYFLASSSGAQRENHGVSSPNSSQGCAQMTGLQSSDKVGPTF SIPSPTFPKSSPTVPEITQPPSYEDAVKQQMTRSQQMDELLDVLIESGEMPADAREDHSCLQKIPKIPGS SCSPTTILPKSSASFEQASSGGQISFDHYATDSEEHLEVLLNSHSPIGKVSDVTLLKIGSEEPPFDGIMD GFPGKAAEDLFSAHELLPGPLSPMHTQLSPPSVDSSGLQLSFTESPWETMEWLDLTPPSSTPGFSNLTSS GPSIFNIDFLDVTDLNLNSPMDLHLQQW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_182667 |
| ORF Size | 2814 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_182667.2, NP_872608.1 |
| RefSeq Size | 4952 bp |
| RefSeq ORF | 2817 bp |
| Locus ID | 246297 |
| UniProt ID | Q8R5I7 |
| MW | 101.9 kDa |
| Gene Summary | Smooth muscle cells (SM) and cardiac muscle cells-specific transcriptional factor which uses the canonical single or multiple CArG boxes DNA sequence. Acts as a cofactor of serum response factor (SRF) with the potential to modulate SRF-target genes (By similarity). Plays a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage (myogenesis) (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN202749 | Myocd (untagged ORF) - Rat transcription factor myocardin (Myocd), (10 ug) |
CNY 12,070.00 |
|
| RR202749L3 | Lenti ORF clone of Myocd (Myc-DDK-tagged ORF) - Rat transcription factor myocardin (Myocd), (10 ug) |
CNY 13,780.00 |
|
| RR202749L4 | Lenti ORF clone of Myocd (mGFP-tagged ORF) - Rat transcription factor myocardin (Myocd), (10 ug) |
CNY 15,200.00 |
