Myo10 (NM_001107657) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR202406 representing NM_001107657 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGTCATGCAATTCAGCAAGGAGGAAGTTCGGGAAGTTCTGAGGTTGCTGGCAGGAATACTGCATC TCGGCAACATAGAATTTATCACTGCTGGAGGGGCACAGATTTCCTTCAAAACGGCTTTGGGGAGGTCGGC AGAGTTACTTGGGCTGGACCCAACACAGCTCACAGATGCGCTGACTCAGAGATCCATGTTCCTCAGGGGA GAAGAGATCCTCACGCCTCTCAGTGTTCAGCAGGCAGTAGATAGCAGAGACTCACTGGCCATGGCACTTT ATGCTCGCTGCTTTGAGTGGGTGATCAAGAAGATCAACAGCCGGATCAAAGGCAAAGACGACTTCAAGTC CATCGGCATCCTTGACATCTTTGGATTTGAAAACTTCGAGGTTAACCACTTTGAGCAGTTCAATATCAAC TATGCAAATGAGAAGCTTCAGGAATACTTCAACAAGCACATTTTTTCTTTAGAACAACTAGAATATAGCA GAGAAGGATTGGTGTGGGAAGATATTGACTGGATAGACAATGGAGAATGCCTAGACTTGATCGAGAAGAA ACTTGGCCTCCTTGCCTTGATCAATGAAGAGAGCCATTTTCCTCAAGCCACAGACAGCACTTTGTTGGAG AAGTTACACAATCAACATGCTAATAACCACTTTTATGTGAAGCCCCGAGTTGCAGTCAACAATTTTGGAG TGAAGCACTATGCAGGAGAGGTGCAGTATGATGTCCGAGGCATCTTGGAAAAGAACAGAGACACGTTCAG AGATGACCTTCTCAACCTGCTCAGGGAAAGCAGATTTGACTTCATCTATGACCTCTTTGAACACATCTCC AGCCGCAACAACCAGGACACCTTGAAATGTGGAAGCAAACACCGGCGGCCTACTGTCAGCTCACAGTTCA AGGACTCCCTGCACTCCTTAATGGCAACACTAAGCTCCTCGAATCCCTTCTTTGTCAGATGCATCAAGCC AAACACGCAGAAGATGCCAGACCAGTTTGATCAGGCCGTCGTGCTCAACCAGCTGCGCTACTCTGGGATG CTGGAGACGGTGAGGATTCGAAAGGCAGGATATGCAGTCCGAAGACCCTTTCAGGATTTTTACAAAAGAT ATAAAGTGCTGATGAGGGACTTGGCTCTGCCTGAGGACATCCGAGGGAAGTGCACTGTGCTCCTGCAGTT CTATGATGCTTCTAACAGCGAGTGGCAACTGGGGAAGACCAAGGTCTTCCTTCGGGAATCCTTGGAGCAG AAACTGGAGAAGAGGCGGGAAGAGGAGATTGACCGGGCAGCCATGGTCATCCGAGCTCACATCTTAGGCT ACTTGGCACGAAAGCAATATAGAAAAGTCCTTTGTGGTGTGGTGACCATACAGAAGAATTACAGAGCTTT CCTCGCGAGGAAGAGATTTCTGCACCTGAAAAAGGCAGCCATCGTGTTCCAGAAGCAGCTCCGAGGACGG CTGGCACGCAAGGTTTACAGGCAGCTTTTGGCCGAGAAACGGGAGCTGGAGGAGAGGAAGAGGTTGGAGG AAGAGAAGAAGAGAGAGGAAGAAGAAAGAGAAAGAAAGCGAGCACAAAGAGAAGCTGACCTCCTCCGTGC CCAGCAGGAGGCAGAAACAAGGAAGCAACAAGAACTCGAGGCCTTGCAGAAGAACCAGAGGGAAGCGGAC CTGACCCGAGAGCTGGAAAAGCAAAGGGAGAACAAGCAGGTGGAAGAGATCCTCCGCCTGGAGAAGGAAA TCGAGGACCTGCAGCGCATGAAGGAGCAGCAGGAGTTGTCGCTGACCGAGGCCTCCCTGCAGAAGCTGCA GCAGCTGCGGGATGAGGAGCTGCGCAGGCTGGAGGACGAGGCGTGTCGAGCTGCCCAGGAGTTCCTAGAG TCCCTCAACTTCGATGAGATCGACGAGTGTGTCCGCAACATCGAGCGCTCCCTGTCCGTCGGCAGCGAGA TTTCAGGTGAGCTCAGCGAGCTTGCAGAGAATGCCAGCGGGGAGAAGCCAAATTTCAACTTCAGCCAGCC CTACCCGGAGGAGGAGGTGGACGAGGGCTTCGAGGCTGATGACGATGCCTTCAAAGACTCACCCAACCCC AGTGAGCATGGCCACTCTGACCAGCGGACCAGTGGCATCCGGACCAGCGACGACTCCTCTGAGGAGGACC CTTACATGAACTACACGGTGGTGCCCACCAGCCCCAGTGCCGACAGCACAGTGCTGCTTGCTGCCTCTGT GCAGGACTCTGCCAGCCTGCACAACTCCTCCAGCGGGGAGTCCACGTACTGCATGCCGCAGAACAACGGG GACCTGCCCTCCCCCGATGGCGACTATGACTATGACCAGGACGATTATGAGGATGGCGCCATTACCTCAG GCAGCAGCGTGACATTCTCCAACTCCTACGGGAGCCAGTGGTCCCCAGACTATAGGTATTCCGTGGGGAC CTACAACAGTTCCGGAGCGTACCGGTTCAGCTCAGAAGGAGCGCAGTCCTCCTTTGAAGATAGTGAAGAG GACTTCGACTCGAGGTTTGATACGGATGATGAGCTCTCCTACCGGCGAGACTCTGTGTATAGCTGCGTCA CACTGCCCTATTTCCACAGTTTCCTGTACATGAAAGGCGGCTTAATGAACTCCTGGAAGCGCCGCTGGTG TGTCCTGAAGGATGAGACCTTCCTGTGGTTCCGCTCCAAGCAGGAAGCCCTCAAGCAAGGCTGGCTGCAC AAGAAAGGCGGGGGCTCCTCCACACTGTCCCGGCGGAACTGGAAGAAGCGCTGGTTTGTTCTCCGCCAGT CCAAGCTGATGTACTTTGAAAACGACAGTGAGGAGAAGCTCAAGGGCACCGTGGAAGTCCGCTCAGCCAA GGAGATCATCGACAACACAAACAAAGAGAACGGGATCGACATCATTATGGCCGACAGGACCTTCCATCTC ATTGCGGAATCCCCCGAAGATGCCAGCCAGTGGTTCAGTGTGCTGAGTCAGGTCCACTCGTCCACAGACC AGGAGATCAGAGAGATGCACGATGAACAGGCAAACCCCCAGAATGCTGTGGGCACACTGGATGTGGGGCT GATTGATTCTGTATGCGCCTCTGACAGCCCAGATAGACCCAACTCATTCGTGATCATCACGGCCAACCGG GTGCTGCACTGCAACGCAGACACACCAGAGGAGATGCACCACTGGATAACACTGCTGCAGAGATCCAAGG GGGACACCAGGGTGGAGGGCCAGGAGTTCATCGTCAGAGGGTGGTTGCATAAGGAAGTGAAGAACAGCCC GAAGATGTCCTCGTTGAAACTGAAGAAGCGGTGGTTCGTGCTGACACACAATTCCCTGGATTACTACAAG AGCTCAGAGAAAAATGCTCTGAAACTGGGCACTCTGGTCCTCAACAGCCTGTGCTCCGTCGTGCCCCCAG ATGAGAAGATATTCAAAGAGACAGGCTACTGGAATGTCACCGTGTATGGGCGCAAACACTGTTACCGACT CTACACGAAACTACTGAATGAGGCGACTCGGTGGTCCAGCGCCATTCAGAATGTGACTGACACCAAGGCT CCAATTGACACGCCCACCCAGCAGCTGATCCAAGATATCAAGGAGAACTGCCTGAACTCTGACGTGGTGG AGCAGATTTACAAGCGGAACCCGATCCTTCGCTACACACATCACCCGCTGCACTCCCCACTCCTGCCTCT TCCCTACGGGGACATAAATCTCAACTTGCTTAAAGACAAAGGCTACACGACCCTCCAGGATGAAGCTATC AAGATATTCAACTCCCTCCAGCAACTGGAGTCCATGTCCGACCCCATCCCTATCATCCAGGGCATCCTGC AGACCGGCCATGACCTGCGGCCTCTGCGGGATGAACTGTACTGTCAGCTCATCAAGCAGACCAATAAGGT GCCCCACCCTGGCAGTGTGGGCAACCTTTACAGCTGGCAGATCCTGACGTGCCTCAGCTGTACCTTCCTG CCAAGCCGGGGCATCCTCAAGTACCTCAAGTTCCACCTGAAAAGGATACGGGAACAGTTCCCAGGAACCG AGATGGAAAAATATGCCCTGTTTATCTACGAATCTCTTAAGAAAACCAAATGCAGAGAGTTTGTGCCTTC CCGAGATGAAATTGAAGCTCTGATCCACCGGCAGGAAATGACATCCACGGTGTACTGCCATGGTGGAGGT TCCTGCAAGATCACCATCAACTCGCACACCACAGCCGGGGAGGTGGTGGAGAAGTTGATCCGGGGCCTCG CCATGGAGGACAGCAGAAATATGTTTGCCTTGTTTGAGTACAATGGACAGGTGGACAAGGCCATCGAAAG CCGGACTATCGTGGCTGATGTCCTGGCAAAGTTTGAAAAGCTGGCTGCCACGTCAGAAGCTGGAGACGCA CCATGGAAATTCTACTTCAAACTTTATTGCTTCCTGGACACCGACAGCATGCCAAAAGACAGCGTGGAGT TTGCATTTATGTTTGAACAGGCCCATGAAGCAGTCATCCATGGCCACCACCCAGCCCCTGAGGAAAGCCT CCAAGTGCTGGCTGCCTTGAGACTCCAGTACCTGCAGGGCGACTATACCCTGCACACCTCAGTCCCACCC CTCGAGGAGGTCTATTCTCTGCAGAGACTCAAAGCTCGCATCAGTCAGTCAACCAAAACCTTCACCCCGT ACGAACGCCTGGAGAAGAGGCGGACCAGCTTCCTGGAGGGGACACTGCGAAGGAGCTTCCGAACCGGGAC GGTGGCTCGGCAGAAGGTGGAGGAGGAGCAGATGCTGGACATGTGGATTAAGGAGGAGATCTGCTCGGCT CGAGCCAGCATCATAGACAAGTGGAAGAAACTGCAGGGAGTGAGCCAGGAACAAGCCATGGCCAAGTATA TGGCCTTGATCAAGGAGTGGCCTGGTTATGGATCAACACTCTTCGACGTGGAGTGCAAAGAAGGCGGCTT CCCCCAGGAGCTCTGGCTGGGCGTCAGCGCAGACGCCGTGTCTGTGTACAAGCGTGGAGAGGGCAAACCG TTAGAAGTCTTCCAGTATGAGCACATCCTCTCTTTCGGGGCACCCCTGGCCAACACATACAAGATTGTGG TTGATGAGAGGGAGCTGCTCTTTGAGACGAGTGAGGTGGTGGACGTGGCCAAGCTCATGAAAGCCTACAT CAGCATGATTGTGAAGAAGCGCTACAGCACCACGCGGTCTCTGAGCAGCCAGGGCAGCTCCAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR202406 representing NM_001107657 Red=Cloning site Green=Tags(s) MEVMQFSKEEVREVLRLLAGILHLGNIEFITAGGAQISFKTALGRSAELLGLDPTQLTDALTQRSMFLRG EEILTPLSVQQAVDSRDSLAMALYARCFEWVIKKINSRIKGKDDFKSIGILDIFGFENFEVNHFEQFNIN YANEKLQEYFNKHIFSLEQLEYSREGLVWEDIDWIDNGECLDLIEKKLGLLALINEESHFPQATDSTLLE KLHNQHANNHFYVKPRVAVNNFGVKHYAGEVQYDVRGILEKNRDTFRDDLLNLLRESRFDFIYDLFEHIS SRNNQDTLKCGSKHRRPTVSSQFKDSLHSLMATLSSSNPFFVRCIKPNTQKMPDQFDQAVVLNQLRYSGM LETVRIRKAGYAVRRPFQDFYKRYKVLMRDLALPEDIRGKCTVLLQFYDASNSEWQLGKTKVFLRESLEQ KLEKRREEEIDRAAMVIRAHILGYLARKQYRKVLCGVVTIQKNYRAFLARKRFLHLKKAAIVFQKQLRGR LARKVYRQLLAEKRELEERKRLEEEKKREEEERERKRAQREADLLRAQQEAETRKQQELEALQKNQREAD LTRELEKQRENKQVEEILRLEKEIEDLQRMKEQQELSLTEASLQKLQQLRDEELRRLEDEACRAAQEFLE SLNFDEIDECVRNIERSLSVGSEISGELSELAENASGEKPNFNFSQPYPEEEVDEGFEADDDAFKDSPNP SEHGHSDQRTSGIRTSDDSSEEDPYMNYTVVPTSPSADSTVLLAASVQDSASLHNSSSGESTYCMPQNNG DLPSPDGDYDYDQDDYEDGAITSGSSVTFSNSYGSQWSPDYRYSVGTYNSSGAYRFSSEGAQSSFEDSEE DFDSRFDTDDELSYRRDSVYSCVTLPYFHSFLYMKGGLMNSWKRRWCVLKDETFLWFRSKQEALKQGWLH KKGGGSSTLSRRNWKKRWFVLRQSKLMYFENDSEEKLKGTVEVRSAKEIIDNTNKENGIDIIMADRTFHL IAESPEDASQWFSVLSQVHSSTDQEIREMHDEQANPQNAVGTLDVGLIDSVCASDSPDRPNSFVIITANR VLHCNADTPEEMHHWITLLQRSKGDTRVEGQEFIVRGWLHKEVKNSPKMSSLKLKKRWFVLTHNSLDYYK SSEKNALKLGTLVLNSLCSVVPPDEKIFKETGYWNVTVYGRKHCYRLYTKLLNEATRWSSAIQNVTDTKA PIDTPTQQLIQDIKENCLNSDVVEQIYKRNPILRYTHHPLHSPLLPLPYGDINLNLLKDKGYTTLQDEAI KIFNSLQQLESMSDPIPIIQGILQTGHDLRPLRDELYCQLIKQTNKVPHPGSVGNLYSWQILTCLSCTFL PSRGILKYLKFHLKRIREQFPGTEMEKYALFIYESLKKTKCREFVPSRDEIEALIHRQEMTSTVYCHGGG SCKITINSHTTAGEVVEKLIRGLAMEDSRNMFALFEYNGQVDKAIESRTIVADVLAKFEKLAATSEAGDA PWKFYFKLYCFLDTDSMPKDSVEFAFMFEQAHEAVIHGHHPAPEESLQVLAALRLQYLQGDYTLHTSVPP LEEVYSLQRLKARISQSTKTFTPYERLEKRRTSFLEGTLRRSFRTGTVARQKVEEEQMLDMWIKEEICSA RASIIDKWKKLQGVSQEQAMAKYMALIKEWPGYGSTLFDVECKEGGFPQELWLGVSADAVSVYKRGEGKP LEVFQYEHILSFGAPLANTYKIVVDERELLFETSEVVDVAKLMKAYISMIVKKRYSTTRSLSSQGSSR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001107657
ORF Size	5244 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001107657.1, NP_001101127.1
RefSeq Size	6862 bp
RefSeq ORF	5247 bp
Locus ID	310178
MW	201.8 kDa
Gene Summary	Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. MYO10 binds to actin filaments and actin bundles and functions as plus end-directed motor. The tail domain binds to membranous compartments containing phosphatidylinositol 3,4,5-trisphosphate or integrins, and mediates cargo transport along actin filaments. Regulates cell shape, cell spreading and cell adhesion. May play a role in neurite outgrowth and axon guidance. In hippocampal neurons it induces the formation of dendritic filopodia by trafficking the actin-remodeling protein VASP to the tips of filopodia, where it promotes actin elongation. Plays a role in formation of the podosome belt in osteoclasts (By similarity). Stimulates the formation and elongation of filopodia.[UniProtKB/Swiss-Prot Function]

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