Ncl (NM_012749) Rat Tagged ORF Clone
CAT#: RR202375
- TrueORF®
Ncl (Myc-DDK-tagged ORF) - Rat nucleolin (Ncl), (10 ug)
"NM_012749" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 9,120.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | MGC93572 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR202375 representing NM_012749
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGAAACTCGCAAAGGCCGGCAAAACCCACGGAGAGTCCAAGAAAATGGCTCCTCCTCCAAAGGAGG TGGAAGAAGATAGTGAGGATGAAGAAATGTCAGAAGATGAAGATGACAGCAGTGGAGAAGAGGAGGTTGT CATCCCTCAGAAGAAAGGCAAAAAGGCTACCACAACTCCAGCAAAGAAGGTGGTTGTTTCACAAACAAAA AAGGCTGCAGTTCCCACACCAGCTAAGAAAGCAGCTGTTACCCCAGGCAAAAAGGCAGCAGCCACACCAG CCAAGAAAGCTGTTACACCAGCCAAAGTAGTTCCAACACCTGGTAAAAAGGGAGCTGCACAAGCAAAAGC ATTGGTACCAACTCCTGGTAAAAAGGGAGCTGTCACTCCAGCCAAGGGGGCTAAGAATGGTAAGAATGCC AAGAAGGAAGACAGCGATGAGGATGAAGATGAAGAGGATGAAGATGACAGCGATGAGGATGAAGATGAAG AGGATGAATTTGAGCCACCGGTAGTAAAAGGAGTGAAACCAGCAAAAGCAGCTCCTGCTGCTCCTGCCTC AGAGGATGAGGATGAGGAAGATGATGATGATGAAGAAGATGATGATGATGATGATGAAGAGGAGGAGGAG GAAGATGACTCTGAGGAAGAAGTTATGGAGATCACACCAGCCAAAGGAAAGAAAACTCCTGCAAAAGTTG TTCCTGTGAAAGCCAAGAGTGTGGCCGAGGAGGAGGAAGATGATGAGGATGATGAAGATGAAGAGGAGGA TGAAGATGAAGAAGATGAAGAGGACGATGAAGATGAGGATGAGGAAGAAGAGGAAGAACCTGTTAAAGCA GCACCTGGAAAACGGAAGAAGGAGATGACCAAGCAGAAAGAAGCCCCTGAAGCCAAGAAACAGAAAATAG AAGGCTCAGAACCAACTACACCTTTCAACCTGTTCATTGGAAACCTTAATCCAAACAAGTCTGTTGCTGA ATTAAAAGTTGCCATCAGTGAACTTTTTGCTAAAAATGATCTTGCTGCTGTGGATGTCAGAACTGGTACA AATAGGAAATTTGGTTATGTTGATTTTGAGTCTGCTGAAGACCTAGAAAAGGCCCTGGAGCTCACTGGTT TAAAAGTGTTTGGCAATGAAATTAAACTAGAAAAACCAAAAGGAAGAGATAGTAAGAAAGTTCGAGCTGC AAGAACACTTTTAGCCAAAAACCTCTCTTTCAACATCACTGAGGATGAATTAAAAGAAGTGTTTGAAGAT GCTGTGGAGATCAGATTAGTCAGCCAGGATGGGAGAAGTAAAGGGATTGCTTATATTGAATTTAAGTCTG AGGCTGATGCAGAGAAAAACTTGGAAGAAAAGCAGGGGGCAGAAATTGATGGACGGTCTGTTTCACTCTA CTACACTGGAGAGAAAGGACAAAGGCAAGAGAGAACTGGAAAGAATAGCACTTGGAGTGGTGAATCAAAG ACTTTGGTTTTAAGTAACCTTTCCTACAGTGCAACAGAAGAAACACTTCAGGAAGTATTCGAGAAAGCAA CCTTTATTAAAGTGCCCCAGAACCCACATGGCAAATCTAAAGGGTATGCATTTATAGAATTTGCTTCATT TGAAGATGCTAAAGAAGCTTTAAATTCCTGTAATAAAATGGAAATTGAGGGCAGAACAATCAGGCTGGAG TTGCAAGGACCCAGGGGATCACCTAATGCGAGAAGTCAGCCATCCAAAACTCTGTTTGTCAAAGGTCTGT CTGAGGATACCACTGAAGAGACCTTAAAAGAATCATTTGAGGGCTCTGTTCGTGCAAGAATAGTAACTGA TCGGGAAACTGGTTCTTCTAAAGGGTTTGGTTTTGTAGACTTTAATAGTGAGGAAGATGCCAAAGCTGCC AAGGAGGCCATGGAAGATGGAGAAATTGATGGAAACAAAGTTACCTTGGACTGGGCCAAACCTAAGGGTG AAGGTGGCTTTGGTGGTCGAGGTGGAGGCAGAGGAGGTTTCGGAGGCAGAGGTGGTGGCAGAGGCGGAAG AGGCGGATTTGGCGGAAGAGGCCGGGGAGGCTTTGGAGGCAGAGGAGGCTTCCGAGGCGGCAGAGGAGGC GGGGGAGACTTCAAGCCACAAGGAAAGAAGACGAAGTTTGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR202375 representing NM_012749
Red=Cloning site Green=Tags(s) MVKLAKAGKTHGESKKMAPPPKEVEEDSEDEEMSEDEDDSSGEEEVVIPQKKGKKATTTPAKKVVVSQTK KAAVPTPAKKAAVTPGKKAAATPAKKAVTPAKVVPTPGKKGAAQAKALVPTPGKKGAVTPAKGAKNGKNA KKEDSDEDEDEEDEDDSDEDEDEEDEFEPPVVKGVKPAKAAPAAPASEDEDEEDDDDEEDDDDDDEEEEE EDDSEEEVMEITPAKGKKTPAKVVPVKAKSVAEEEEDDEDDEDEEEDEDEEDEEDDEDEDEEEEEEPVKA APGKRKKEMTKQKEAPEAKKQKIEGSEPTTPFNLFIGNLNPNKSVAELKVAISELFAKNDLAAVDVRTGT NRKFGYVDFESAEDLEKALELTGLKVFGNEIKLEKPKGRDSKKVRAARTLLAKNLSFNITEDELKEVFED AVEIRLVSQDGRSKGIAYIEFKSEADAEKNLEEKQGAEIDGRSVSLYYTGEKGQRQERTGKNSTWSGESK TLVLSNLSYSATEETLQEVFEKATFIKVPQNPHGKSKGYAFIEFASFEDAKEALNSCNKMEIEGRTIRLE LQGPRGSPNARSQPSKTLFVKGLSEDTTEETLKESFEGSVRARIVTDRETGSSKGFGFVDFNSEEDAKAA KEAMEDGEIDGNKVTLDWAKPKGEGGFGGRGGGRGGFGGRGGGRGGRGGFGGRGRGGFGGRGGFRGGRGG GGDFKPQGKKTKFE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_012749 |
ORF Size | 2142 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_012749.2, NP_036881.2 |
RefSeq Size | 2501 bp |
RefSeq ORF | 2145 bp |
Locus ID | 25135 |
MW | 77.3 kDa |
Gene Summary | nucleolar-specific protein; involved in cell growth [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN202375 | Ncl (untagged ORF) - Rat nucleolin (Ncl), (10 ug) |
CNY 9,120.00 |
|
RR202375L3 | Lenti ORF clone of Ncl (Myc-DDK-tagged ORF) - Rat nucleolin (Ncl), (10 ug) |
CNY 10,930.00 |
|
RR202375L4 | Lenti ORF clone of Ncl (mGFP-tagged ORF) - Rat nucleolin (Ncl), (10 ug) |
CNY 12,070.00 |