Actc1 (NM_019183) Rat Tagged ORF Clone
CAT#: RR202192
- TrueORF®
Actc1 (Myc-DDK-tagged ORF) - Rat actin, alpha, cardiac muscle 1 (Actc1), (10 ug)
"NM_019183" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 5,488.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | MGC156490 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR202192 representing NM_019183
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGTGACGACGAGGAGACCACAGCTTTGGTGTGCGACAATGGCTCCGGACTGGTGAAGGCTGGCTTTG CGGGTGATGATGCTCCCAGAGCTGTCTTCCCATCCATCGTGGGTCGCCCACGCCACCAGGGTGTCATGGT AGGTATGGGGCAGAAAGACTCCTATGTAGGTGACGAGGCTCAGAGCAAGCGAGGCATCCTGACTCTGAAG TACCCCATAGAGCACGGCATTATCACCAACTGGGACGACATGGAGAAGATCTGGCACCACACCTTCTACA ATGAGCTCCGTGTGGCCCCTGAGGAGCACCCGACCCTGCTCACTGAGGCCCCACTGAACCCCAAGGCCAA CCGTGAGAAGATGACACAGATCATGTTTGAGACCTTCAATGTGCCTGCCATGTATGTGGCCATCCAGGCG GTGCTGTCCCTGTACGCTTCTGGGAGAACCACAGGCATTGTTCTGGATTCTGGCGACGGTGTGACTCATA ACGTCCCCATCTATGAGGGTTATGCCTTACCCCATGCCATCATGCGTCTGGATCTAGCTGGTCGGGACCT CACTGACTACCTTATGAAGATCCTCACTGAGCGCGGGTACTCCTTTGTCACCACCGCTGAACGTGAAATT GTCCGAGACATCAAAGAAAAACTGTGTTATGTCGCCCTGGATTTTGAGAATGAGATGGCCACCGCTGCAT CTTCCTCCTCTCTGGAGAAGAGCTACGAACTTCCCGATGGCCAGGTCATCACCATTGGCAACGAGCGTTT CCGCTGTCCTGAGACTCTCTTCCAGCCCTCTTTCATTGGTATGGAGTCTGCCGGCATTCACGAAACTACT TATAACAGCATCATGAAGTGTGACATCGATATCCGCAAAGACCTGTACGCCAACAATGTCCTGTCTGGAG GCACCACCATGTACCCTGGGATTGCTGATCGTATGCAAAAGGAAATAACTGCACTGGCTCCCAGCACCAT GAAGATTAAGATTATTGCTCCCCCTGAGCGTAAATACTCTGTCTGGATTGGGGGCTCCATCCTGGCCTCT CTGTCTACCTTCCAACAAATGTGGATCAGCAAGCAAGAGTACGATGAGGCCGGGCCCTCCATTGTCCACC GCAAGTGCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR202192 representing NM_019183
Red=Cloning site Green=Tags(s) MCDDEETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLK YPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQA VLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREI VRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETT YNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILAS LSTFQQMWISKQEYDEAGPSIVHRKCF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_019183 |
ORF Size | 1131 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_019183.1, NP_062056.1 |
RefSeq Size | 1489 bp |
RefSeq ORF | 1134 bp |
Locus ID | 29275 |
UniProt ID | P68035 |
MW | 42.5 kDa |
Gene Summary | may act as a structural component of the cytoskeleton; may play a role in muscle function [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN202192 | Actc1 (untagged ORF) - Rat actin, alpha, cardiac muscle 1 (Actc1), (10 ug) |
CNY 5,488.00 |
|
RR202192L3 | Lenti ORF clone of Actc1 (Myc-DDK-tagged ORF) - Rat actin, alpha, cardiac muscle 1 (Actc1), (10 ug) |
CNY 6,080.00 |
|
RR202192L4 | Lenti ORF clone of Actc1 (mGFP-tagged ORF) - Rat actin, alpha, cardiac muscle 1 (Actc1), (10 ug) |
CNY 6,650.00 |