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Pnck (NM_017275) Rat Tagged ORF Clone
CAT＃: RR202126
- TrueORF®
Pnck (Myc-DDK-tagged ORF) - Rat pregnancy upregulated non-ubiquitously expressed CaM kinase (Pnck), (10 ug)
"NM_017275" in other vectors (3)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Camk1b |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR202126 representing NM_017275
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCTGCTCAAGAAACAGACGGAGGACATCAGCAGTGTCTATGAGATCCGGGAGAAGCTGGGCTCGG GTGCCTTCTCTGAGGTGATGCTGGCCCAGGAAAGGGGCTCTGCTCATCTTGTGGCCCTCAAGTGCATTCC CAAGAAAGCACTTCGGGGCAAGGAGGCCCTGGTGGAGAATGAGATCGCAGTACTCCGCAGGATTAGCCAC CCCAACATTGTGGCTCTGGAGGACGTCCACGAGAGCCCTTCCCATCTCTACTTGGCCATGGAGCTGGTAA CAGGTGGTGAACTGTTTGACCGAATCATGGAGCGGGGCTCCTACACAGAGAAGGATGCGAGCCACCTTGT AGGGCAGGTCCTTGGTGCTGTCTCCTACCTTCATAGCCTGGGCATCGTGCACCGGGACCTCAAGCCTGAA AACCTCCTCTATGCCACACCTTTTGAGGACTCCAAGATCATGGTCTCTGACTTTGGCCTGTCCAAAATTC AAGCTGGCAACATGCTAGGCACAGCCTGTGGGACCCCAGGATATGTGGCCCCAGAGCTCCTGGAGCAGAA ACCCTACGGGAAGGCCGTAGATGTGTGGGCCCTGGGTGTCATCTCCTACATCCTGCTGTGTGGGTACCCC CCCTTCTATGATGAGAGCGATCCTGAACTCTTCAGCCAGATTCTGAGGGCCAGCTACGAGTTTGACTCTC CCTTTTGGGATGACATCTCAGAATCAGCCAAAGACTTCATTCGGCACCTTCTGGAACGTGATCCCCAGAA GAGGTTCACCTGCCAACAGGCCTTACAGCATCTCTGGATCTCTGGGGATGCAGCCTTGGACAGGGACATC CTAGGTTCTGTCAGTGAGCAGATCCAGAAGAATTTTGCCAGGACCCACTGGAAGCGTGCATTCAATGCCA CATCATTCCTACGTCACATCCGTAAGCTGGGACAGAGCCCAGAGGGTGAGGAGGCCTCCAGGCAGGGTAT GACCCGTCACAGCCACCCAGGCCTTGGGACTAGCCAGTCTCCCAAGTGGGACCCGTCACAGCCACCCAGG CCTTGGGACTAGCCAGTCTCCCAAGTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR202126 representing NM_017275
Red=Cloning site Green=Tags(s) MLLLKKQTEDISSVYEIREKLGSGAFSEVMLAQERGSAHLVALKCIPKKALRGKEALVENEIAVLRRISH PNIVALEDVHESPSHLYLAMELVTGGELFDRIMERGSYTEKDASHLVGQVLGAVSYLHSLGIVHRDLKPE NLLYATPFEDSKIMVSDFGLSKIQAGNMLGTACGTPGYVAPELLEQKPYGKAVDVWALGVISYILLCGYP PFYDESDPELFSQILRASYEFDSPFWDDISESAKDFIRHLLERDPQKRFTCQQALQHLWISGDAALDRDI LGSVSEQIQKNFARTHWKRAFNATSFLRHIRKLGQSPEGEEASRQGMTRHSHPGLGTSQSPKWDPSQPPR PWD*PVSQV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017275 |
| ORF Size | 1029 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq Size | 1618 bp |
| RefSeq ORF | 1032 bp |
| Locus ID | 29660 |
| UniProt ID | O70150 |
| MW | 38.4 kDa |
| Gene Summary | contains alternatively spliced isoforms CaM kinase Ibeta3 and Ibeta1; both isoforms activate CaM kinase Ialpha activity via Thr177 phosphorylation [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN202126 | Pnck (untagged ORF) - Rat pregnancy upregulated non-ubiquitously expressed CaM kinase (Pnck), (10 ug) |
CNY 3,990.00 |
|
| RR202126L3 | Lenti ORF clone of Pnck (Myc-DDK-tagged ORF) - Rat pregnancy upregulated non-ubiquitously expressed CaM kinase (Pnck), (10 ug) |
CNY 6,080.00 |
|
| RR202126L4 | Lenti ORF clone of Pnck (mGFP-tagged ORF) - Rat pregnancy upregulated non-ubiquitously expressed CaM kinase (Pnck), (10 ug) |
CNY 6,650.00 |
