Cand1 (NM_054004) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Tip120; Tip120A
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR201857 representing NM_054004 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAGCGCTTCGTACCACATCTCCAACTTGCTGGAAAAAATGACATCCAGCGACAAGGACTTCAGGT TTATGGCTACAAATGATCTGATGACAGAACTGCAGAAAGACTCCATCAAGCTGGATGATGACAGCGAAAG GAAAGTCGTAAAGATGATTCTGAAGTTGCTGGAGGATAAAAATGGCGAAGTGCAGAACTTAGCTGTGAAA TGTCTTGGTCCTTTAGTGAGTAAAGTGAAAGAGTACCAAGTTGAGACGATTGTAGACACCCTCTGTACAA ACATGCTTTCTGACAAGGAGCAGCTTCGAGATATTTCCAGTATTGGCCTTAAAACAGTAATTGGAGAACT CCCTCCAGCTTCCAGTGGCTCTGCATTAGCTGCTAATGTATGTAAAAAGATTACTGGACGCCTTACCAGT GCAATAGCAAAGCAGGAAGATGTTTCTGTTCAGCTAGAAGCTTTAGATATTATGGCTGATATGTTGAGCA GGCAAGGAGGACTGCTTGTTAATTTCCATCCTTCAATTCTGACCTGTCTACTTCCCCAGCTGACTAGCCC TAGACTTGCAGTCAGGAAAAGAACAATTATTGCTCTTGGCCACCTAGTTATGAGCTGCGGAAACATAGTT TTTGTAGACCTTATTGAACATCTGTTGTCAGAGTTGTCCAAAAATGACTCCATGTCAACAACAAGGACCT ACATACAGTGTATTGCTGCTATTAGTAGGCAAGCTGGCCATAGAATAGGTGAATACCTTGAAAAGATAAT TCCTTTGGTGGTAAAATTTTGTAATGTAGATGATGATGAATTAAGAGAATACTGCATTCAAGCTTTTGAA TCATTTGTGAGAAGATGCCCTAAGGAAGTTTATCCTCATGTTTCTACCATTATAAACATTTGTCTAAAAT ACCTTACCTATGATCCAAATTACAACTATGATGATGAAGATGAAGATGAGAATGCTATGGATGCTGATGG CGGAGATGACGATGACCAAGGGAGTGACGATGAATACAGTGATGACGATGACATGAGTTGGAAAGTGAGG CGTGCGGCTGCCAAGTGCCTGGATGCCGTAGTTAGCACACGGCATGAGATGCTCCCGGAATTCTACAAGA CCGTCTCTCCTGCACTGATATCCAGATTTAAAGAGCGCGAAGAGAATGTAAAGGCAGATGTTTTTCATGC ATACCTTTCTCTTTTGAAGCAAACTCGTCCAGTACAAAGTTGGCTGTGCGACCCTGATGCAATGGAGCAA GGAGAAACACCCTTAACAATGCTTCAGAGTCAGGTTCCCAACATTGTTAAAGCCCTACATAAACAGATGA AAGAAAAAAGTGTAAAGACCCGACAGTGTTGTTTTAACATGTTAACTGAACTGGTAAATGTGTTGCCTGG GGCACTAACCCAACACATTCCTGTACTTGTACCAGGAATCATTTTCTCACTCAATGATAAGTCCAGCTCA TCGAATCTGAAGATTGATGCATTGTCCTGTCTGTATGTGATCCTCTGTAACCACTCTCCGCAAGTTTTCC ACCCGCATGTTCAGGCTTTGGTCCCTCCAGTGGTGGCTTGTGTTGGTGACCCATTTTACAAGATCACATC AGAAGCCCTTCTTGTCACTCAGCAGCTTGTCAAAGTAATCCGTCCTCTGGACCAACCCTCCTCCTTCGAT GCAACGCCTTACATCAAAGATCTCTTCACTTGTACAATTAAGCGCTTAAAAGCAGCTGACATTGATCAGG AAGTCAAGGAGAGGGCTATTTCCTGTATGGGACAGATTATTTGCAATCTTGGAGACAATTTGGGCCCTGA CTTATCAAATACACTTCAGATTTTCTTGGAGAGACTCAAGAATGAAATCACCCGGCTAACGACAGTGAAA GCACTGACCCTGATTGCTGGGTCACCTTTGAAGATAGATCTGAGGCCTGTGCTGGGAGAGGGAGTCCCTA TCCTTGCTTCATTTCTCAGGAAAAATCAGAGAGCTTTGAAACTGGGGACCCTCTCTGCCCTAGATATTCT CATTAAAAACTATAGTGACAGTTTGACGGCCGCCATGATTGATGCAGTTCTGGATGAGCTCCCTCCTCTT ATCAGCGAAAGTGATATGCACGTGTCCCAGATGGCTATCAGCTTCCTTACTACCCTGGCAAAAGTATATC CCTCCTCCCTTTCAAAGATAAGCGGATCTATTCTCAATGAACTGATTGGACTTGTAAGGTCACCTCTGCT GCAGGGAGGAGCTCTTAGTGCCATGCTAGACTTCTTCCAAGCTTTGGTTGTCACTGGAACAAACAATCTA GGATACATGGATTTGCTGCGCATGCTAACGGGTCCAGTTTACTCTCAGAGCACAGCTCTTACTCATAAGC AGTCTTACTATTCCATTGCCAAATGTGTAGCTGCCCTTACTCGAGCATGCCCTAAAGAGGGACCGGCTGT AGTAGGTCAGTTTATTCAAGATGTCAAGAACTCCAGGTCTACAGATTCCATTCGTCTCTTAGCGCTCCTT TCTCTAGGAGAAGTTGGACACCATATTGACTTAAGTGGGCAGTTGGAGCTAAAATCTGTAATATTAGAGG CTTTCTCCTCTCCTAGTGAAGAAGTTAAATCAGCTGCATCCTATGCGTTAGGCAGCATCAGTGTAGGCAA CCTCCCTGAGTATCTGCCATTTGTACTACAAGAAATAACCAGTCAGCCCAAAAGGCAGTACCTGCTGCTG CATTCCCTGAAGGAAATCATTAGCTCTGCGTCAGTGGTCGGCCTTAAGCCGTATGTCGAGAACATCTGGG CCTTGCTGCTAAAGCACTGTGAGTGCGCGGAGGAGGGCACCAGAAACGTCGTCGCCGAGTGTCTAGGGAA GCTCACTCTTATTGATCCTGAAACTCTCCTCCCGAGGCTTAAAGGGTATTTGATATCAGGGTCATCCTAT GCCAGAAGCTCAGTGGTTACAGCTGTGAAGTTCACTATTTCTGACCACCCTCAGCCCATTGATCCACTGC TCAAGAACTGCATAGGTGATTTTCTAAAAACGTTGGAAGACCCAGATTTGAATGTAAGAAGAGTGGCCTT GGTCACATTCAATTCTGCAGCCCATAACAAGCCGTCACTGATACGGGACCTTCTGGATTCTGTTCTTCCA CATCTTTACAATGAGACAAAAGTTAGGAAGGAACTTATAAGAGAGGTAGAGATGGGCCCGTTTAAGCACA CGGTTGATGACGGCCTGGACATTAGAAAGGCAGCTTTTGAGTGTATGTACACACTTCTAGACAGCTGTCT TGACAGACTGGATATCTTTGAATTTCTAAATCATGTGGAAGATGGTTTGAAGGACCATTATGATATTAAG ATGCTAACATTTTTAATGTTGGTGAGACTATCTACCCTTTGTCCAAGTGCAGTACTACAGAGGTTGGACC GGCTTGTTGAGCCACTACGAGCTACGTGTACAACTAAGGTGAAGGCGAACTCTGTAAAGCAGGAGTTTGA AAAGCAGGACGAGTTAAAGCGCTCGGCCATGAGGGCAGTAGCTGCACTGCTCACCATTCCAGAGGCAGAG AAGAGCCCTCTCATGAGTGAGTTCCAGTCACAGATCAGCTCCAACCCGGAGCTGGCAGCCATCTTTGAAA GTATCCAGAAAGATTCTTCGTCCACTAACTTGGAATCAATGGACACTAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR201857 representing NM_054004 Red=Cloning site Green=Tags(s) MASASYHISNLLEKMTSSDKDFRFMATNDLMTELQKDSIKLDDDSERKVVKMILKLLEDKNGEVQNLAVK CLGPLVSKVKEYQVETIVDTLCTNMLSDKEQLRDISSIGLKTVIGELPPASSGSALAANVCKKITGRLTS AIAKQEDVSVQLEALDIMADMLSRQGGLLVNFHPSILTCLLPQLTSPRLAVRKRTIIALGHLVMSCGNIV FVDLIEHLLSELSKNDSMSTTRTYIQCIAAISRQAGHRIGEYLEKIIPLVVKFCNVDDDELREYCIQAFE SFVRRCPKEVYPHVSTIINICLKYLTYDPNYNYDDEDEDENAMDADGGDDDDQGSDDEYSDDDDMSWKVR RAAAKCLDAVVSTRHEMLPEFYKTVSPALISRFKEREENVKADVFHAYLSLLKQTRPVQSWLCDPDAMEQ GETPLTMLQSQVPNIVKALHKQMKEKSVKTRQCCFNMLTELVNVLPGALTQHIPVLVPGIIFSLNDKSSS SNLKIDALSCLYVILCNHSPQVFHPHVQALVPPVVACVGDPFYKITSEALLVTQQLVKVIRPLDQPSSFD ATPYIKDLFTCTIKRLKAADIDQEVKERAISCMGQIICNLGDNLGPDLSNTLQIFLERLKNEITRLTTVK ALTLIAGSPLKIDLRPVLGEGVPILASFLRKNQRALKLGTLSALDILIKNYSDSLTAAMIDAVLDELPPL ISESDMHVSQMAISFLTTLAKVYPSSLSKISGSILNELIGLVRSPLLQGGALSAMLDFFQALVVTGTNNL GYMDLLRMLTGPVYSQSTALTHKQSYYSIAKCVAALTRACPKEGPAVVGQFIQDVKNSRSTDSIRLLALL SLGEVGHHIDLSGQLELKSVILEAFSSPSEEVKSAASYALGSISVGNLPEYLPFVLQEITSQPKRQYLLL HSLKEIISSASVVGLKPYVENIWALLLKHCECAEEGTRNVVAECLGKLTLIDPETLLPRLKGYLISGSSY ARSSVVTAVKFTISDHPQPIDPLLKNCIGDFLKTLEDPDLNVRRVALVTFNSAAHNKPSLIRDLLDSVLP HLYNETKVRKELIREVEMGPFKHTVDDGLDIRKAAFECMYTLLDSCLDRLDIFEFLNHVEDGLKDHYDIK MLTFLMLVRLSTLCPSAVLQRLDRLVEPLRATCTTKVKANSVKQEFEKQDELKRSAMRAVAALLTIPEAE KSPLMSEFQSQISSNPELAAIFESIQKDSSSTNLESMDTS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_054004
ORF Size	3690 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_054004.2, NP_446456.1
RefSeq Size	4381 bp
RefSeq ORF	3693 bp
Locus ID	117152
UniProt ID	P97536
MW	136.4 kDa
Gene Summary	interacts with TATA-binding protein and may be involved in transcription regulation [RGD, Feb 2006]

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